Progress of mesenchymal stem cells (MSCs) & MSC-Exosomes combined with drugs intervention in liver fibrosis

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 176, P. 116848 - 116848

Published: June 3, 2024

Liver fibrosis is an intrahepatic chronic damage repair response caused by various reasons such as alcoholic liver, fatty viral hepatitis, autoimmune diseases, etc., and closely related to the progression of liver disease. Currently, mechanisms its treatment are hot research topics in field disease remedy. Mesenchymal stem cells (MSCs) a class adult with self-renewal multidirectional differentiation potential, which can ameliorate through hepatic-directed differentiation, paracrine effects, immunomodulation. However, low inner-liver colonization rate, survival short duration intervention after cell transplantation have limited their wide clinical application. With intensive on worldwide, it has been found that MSCs MSCs-derived exosomes combined drugs shown better efficiency than utilization alone many animal models fibrosis. In this paper, we review interventional effects mesenchymal alleviate hepatic vivo recent years, will provide new ideas improve efficacy treating clinic.

Language: Английский

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Cutting-edge insights into liver fibrosis: advanced therapeutic strategies and future perspectives using engineered mesenchymal stem cell-derived exosomes

Drug Delivery and Translational Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 24, 2025

Abstract Liver fibrosis is still a serious health concern worldwide, and there increasing interest in mesenchymal stem cells (MSCs) with tremendous potential for treating this disease because of their regenerative paracrine effects. Recently, many researches have focused on using the released exosomes (EXOs) from to treat liver rather than parent themselves. MSC-derived EXOs (MSC-EXOs) demonstrated favourable outcomes similar cell treatment terms regenerative, immunomodulatory, anti-apoptotic, anti-oxidant, anti-necroptotic, anti-inflammatory anti-fibrotic actions several models fibrosis. are superior terms, including lower immunogenicity risk tumour formation. However, maintaining stability efficacy after vivo transplantation remains major challenge clinical applicability. Therefore, strategies been applied engineering, such as parental modification or modifying directly achieve optimum performance Herein, we discuss underlying mechanisms an overview available therapies, among them EXOs. We also summarise recent developments improving effectiveness advantages limitations these approaches upcoming applications. Graphical

Language: Английский

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Hotspots and trends in stem cell therapy for liver fibrosis and cirrhosis: A bibliometric analysis

World Journal of Hepatology, Journal Year: 2025, Volume and Issue: 17(1)

Published: Jan. 6, 2025

Liver fibrosis and cirrhosis are global medical challenges that require safe effective treatments. In the past two decades, there has been a surge in research on stem cell therapy for liver cirrhosis. This study aimed to conduct comprehensive analysis of hotspots trends this field through bibliometrics. To bibliometric treatment Publications were retrieved from Web Science Core Collection database. The distribution collaboration among literature, authors, countries, institutions analyzed visually using Excel, CiteSpace, Bibliometrix R-package, VOSviewer Pajek software. Additionally, an investigation keywords, burst clusters was conducted. As September 20, 2024, total 1935 documents dating 2004 with 1186 strongly relevant publications obtained after screening. China, United States, Japan major contributors field. Cairo University, Zhejiang University Yamaguchi institution journal Stem Cell Research & Therapy published most papers. There 686 Shuji Terai, Isao Sakaida, Soon Koo Baik, Lanjuan Li publishing focused alcoholic nonalcoholic fatty disease. emerging areas interest extracellular vesicles, exosomes, their enriched microRNAs. is experiencing rapid growth due changing increasing literature. These findings provide thorough overview

Language: Английский

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Platelet-Activating Factor-Induced Inflammation in Obesity: A Two-Sided Coin of Protection and Risk

Cells, Journal Year: 2025, Volume and Issue: 14(7), P. 471 - 471

Published: March 21, 2025

Obesity, marked by excessive fat accumulation, especially abdominal, is a global health concern with significant public impact. While obesity-associated chronic unresolved inflammation contributes to metabolic dysfunctions, acute supports healthy adipose tissue remodeling and expansion. Platelet-activating factor (PAF), “primitive” signaling molecule, among the key mediators involved in phase of various pathophysiological processes. This article explores role PAF accumulation obesity reviewing experimental data from cell cultures, animals, humans. It proposes an emerging biochemical mechanism attempt explain its dual obese tissue, including also on PAF’s potential involvement epigenetic mechanisms that may be linked “obesity memory”. Finally, it highlights natural modulators promoting functional thermogenesis, prevention through lifestyle, Mediterranean diet rich weak agonists/PAF receptor antagonists regular exercise, which help maintain controlled levels. Conversely, cases obesity-related systemic levels, potent inhibitors like ginkgolide B rupatadine mitigate dysfunctions PAFR potentially enhancing their effects synergistically.

Language: Английский

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Extracellular vesicles: emerging therapeutic agents for liver fibrosis

Extracellular Vesicles and Circulating Nucleic Acids, Journal Year: 2025, Volume and Issue: 6(2), P. 216 - 44

Published: May 7, 2025

Liver fibrosis is a progressive condition characterized by excessive scar tissue buildup, leading to impaired liver function and potentially cirrhosis. Despite advancements in treatment strategies, effective anti-fibrotic therapies remain an urgent unmet need. Recent research has identified extracellular vesicles (EVs) as promising therapeutic agents due their ability mediate intercellular communication regulate key fibrotic pathways. This review aims provide comprehensive overview of the potential EVs different vitro vivo models hepatic fibrosis, focusing on natural effects recent engineering for enhanced efficacy. can be derived from various cellular sources, including mesenchymal stromal cells (MSCs) liver-resident cells. Biological materials, serum, breast milk, bacteria, plants, also serve EV sources. Among these, MSC-EVs stand out potential, which significantly through preconditioning with inflammatory signals, pharmacological agents, or genetic improve quality Engineering techniques have further expanded applications, enabling use precise drug-delivery vehicles. Approaches such loading compounds, designing customized EVs, creating EV-liposome hybrids enable targeted delivery activated stellate (HSCs), central drivers progression. These strategies enhance efficacy EV-based treatments. Both engineered critical pathways development, activation HSCs, modulation pro-fibrotic genes, matrix deposition, programmed cell death. Additionally, modulate immune responses, fostering microenvironment conducive repair regeneration. Combining regenerative properties innovative provides highly targeted, approaches restore address need chronic disease therapies.

Language: Английский

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Comparative Analysis of Exosomes and Extracellular Microvesicles in Healing Pathways: Insights for Advancing Regenerative Therapies

Molecules, Journal Year: 2024, Volume and Issue: 29(15), P. 3681 - 3681

Published: Aug. 3, 2024

Exosomes and microvesicles bear great potential to broaden therapeutic options in the clinical context. They differ genesis, size, cargo, composition despite their similarities. were identified as participating various processes such angiogenesis, cell migration, intracellular communication. Additionally, they are characterized by natural biocompatibility. Therefore, researchers concluded that could serve a novel curative method capable of achieving unprecedented results. Indeed, experiments, proved remarkably efficient enhancing wound regeneration mitigating inflammation. Despite immense advancements research on exosomes microvesicles, time for large-scale application is yet come. This article aims gather analyze current knowledge those promising particles, characteristics, implementations.

Language: Английский

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Single and combined strategies for mesenchymal stem cell exosomes alleviate liver fibrosis: a systematic review and meta-analysis of preclinical animal models

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: July 31, 2024

The efficacy of mesenchymal stem cells (MSCs) in treating liver fibrosis has been supported by various clinical studies. However, cell transplantation is limited application due to its low survival rate, implantation and possible carcinogenicity. Recently, there increasing interest the use MSC-exos their widespread availability, immunogenicity, non-carcinogenic properties. Numerous studies have demonstrated potential preventing progression end-stage disease.

Language: Английский

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Innovative preconditioning strategies for improving the therapeutic efficacy of extracellular vesicles derived from mesenchymal stem cells in gastrointestinal diseases

Inflammopharmacology, Journal Year: 2023, Volume and Issue: 31(6), P. 2973 - 2993

Published: Oct. 24, 2023

Abstract Gastrointestinal (GI) diseases have become a global health issue and an economic burden due to their wide distribution, late prognosis, the inefficacy of recent available medications. Therefore, it is crucial search for new strategies management. In decades, mesenchymal stem cells (MSCs) therapy has attracted attention as viable option treating myriad GI disorders such hepatic fibrosis (HF), ulcerative colitis (UC), acute liver injury (ALI), non-alcoholic fatty disease (NAFLD) regenerative paracrine properties. Importantly, studies shown that MSC-derived extracellular vesicles (MSC-EVs) are responsible most therapeutic effects MSCs. addition, EVs revealed several benefits over parent MSCs, being less immunogenic, having lower risk tumour formation, able cross biological barriers, easier store. MSC-EVs exhibited regenerative, anti-oxidant, anti-inflammatory, anti-apoptotic, anti-fibrotic in different experimental models diseases. However, key with clinical application maintenance stability efficacy following vivo transplantation. Preconditioning or one novel methods used improve effectiveness stability. Herein, we discuss taking into account mechanism action. We also summarise challenges restrictions need be overcome promote treatment various well developments effectiveness. Graphical abstract A representation innovative preconditioning techniques been suggested improving The pathological conditions (ALI, UC, HF NAFLD) create harsh environment parents, increasing apoptosis senescence MSCs thereby diminishing yield restricting large-scale applications. pharmacological agents mediators can through adaption lethal which they subjected. This result establishment more conducive activation numerous vital trajectories act immunomodulatory, reparative activities derived EVs, part system. ALI, injury; diseases, gastrointestinal diseases; HF, fibrosis; HSP, heat shock protein; miRNA, microRNA; mRNA, messenger RNA; MSC-EVs, cell-derived vesicles; NAFLD, disease; colitis.

Language: Английский

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Inhibition of Adipocyte Necroptosis Alleviates Fat Necrosis and Fibrosis after Grafting: In a Mice Model

Aesthetic Surgery Journal, Journal Year: 2024, Volume and Issue: 44(8), P. NP585 - NP605

Published: May 24, 2024

Because of the delicate structure adipose tissue, fat necrosis accounts for 43.7% all complications after autologous grafting; however, its regulation remains unclear.

Language: Английский

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Native and engineered extracellular vesicles: novel tools for treating liver disease

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(16), P. 3840 - 3856

Published: Jan. 1, 2024

Liver diseases are classified as acute liver damage and chronic disease, with recurring causing fibrosis progression to cirrhosis hepatoma. transplantation is the only effective treatment for end-stage diseases; therefore, novel therapies required. Extracellular vesicles (EVs) endogenous nanocarriers involved in cell-to-cell communication that play important roles immune regulation, tissue repair regeneration. Native EVs can potentially be used various owing their high biocompatibility, low immunogenicity permeability engineered surface modification or cargo loading could further optimize therapeutic effects. In this review, we firstly introduced mechanisms effects of native derived from different cells tissues treat etiologies. Additionally, summarized possible methods facilitate targeting improve cargo-loading efficiency. detailed latest delivery strategies were also discussed. Finally, pointed out limitations challenges future development applications. We hope review provide a useful reference promote clinical translation.

Language: Английский

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