Bacterial-Mediated In Situ Engineering of Tumour-Associated Macrophages for Cancer Immunotherapy

Cancers, Journal Year: 2025, Volume and Issue: 17(5), P. 723 - 723

Published: Feb. 20, 2025

Background/Objectives: Tumour-associated macrophages (TAMs) are critical components of the tumour microenvironment (TME), significantly influencing cancer progression and treatment resistance. This review aims to explore innovative use engineered bacteria reprogram TAMs, enhancing their anti-tumour functions improving therapeutic outcomes. Methods: We conducted a systematic following predefined protocol. Multiple databases were searched identify relevant studies on phenotypic plasticity, for reprogramming. Inclusion exclusion criteria applied select studies, data extracted using standardised forms. Data synthesis was performed summarise findings, focusing mechanisms benefits non-pathogenic modify TAMs. Results: The summarises findings that can selectively target promoting shift from tumour-promoting M2 phenotype tumour-fighting M1 phenotype. reprogramming enhances pro-inflammatory responses activity within TME. Evidence various indicates significant regression improved immune bacterial therapy. Conclusions: Reprogramming TAMs presents promising strategy approach leverages natural targeting abilities directly tumour, potentially patient outcomes offering new insights into immune-based treatments. Further research is needed optimise these methods assess clinical applicability.

Language: Английский

Unveiling the microbial influence: bacteria’s dual role in tumor metastasis

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15

Published: March 14, 2025

As cancer research advances, the intricate relationship between microbiome and is gaining heightened recognition, especially concerning tumor metastasis, where bacterial involvement becomes increasingly complex. This review seeks to systematically examine dual roles of bacteria in metastasis process, encompassing both mechanisms that facilitate inhibitory effects exerted by specific microorganisms. We explore through which influence cell migration inducing chronic inflammation, evading host immune responses, remodeling ECM. Moreover, immunomodulatory potential probiotics genetically engineered offers promising prospects for prevention treatment metastasis. article elucidates complexity emerging frontiers examining clinical significance as biomarkers evaluating antibiotic usage on metastatic process. posit comprehending biological characteristics bacteria, a critical component microenvironment, will offer innovative strategies theoretical foundations treatment. Furthermore, this explores future directions, including application technologies bacteria-based therapeutic strategies, thereby offering valuable perspective development novel anti-cancer approaches.

Language: Английский

Organoids with a Type 1 Collagen Scaffold to Model Bacterial Cancer Therapy

Cells, Journal Year: 2025, Volume and Issue: 14(7), P. 524 - 524

Published: April 1, 2025

Bacterial cancer therapy (BCT) is emerging as an important option for the treatment of solid tumours, with promising outcomes in preclinical trials. Further progress hampered by incomplete understanding how oncotropic bacteria, such attenuated strains Salmonella enterica serovar Typhimurium, colonise tumours and responses both bacteria tumour cells to this colonisation. To model this, we developed organoids that are permissive bacterial colonisation, replacing conventional commercially available extracellular matrix (e.g., Matrigel) a type I collagen scaffold. A comparison two matrices indicated 1 permitted initial infection efficiency more than 5-times greater Matrigel. In addition, subsequent growth within expanded cell numbers over 10-fold 4 days infection. These allow visualisation chemoattraction, invasion population interior lumen, will permit future optimisation BCT. establishing patient-derived organoids, demonstrate platform developing personalised treatments exploiting

Language: Английский