miR-181a/MSC-Loaded Nano-Hydroxyapatite/Collagen Accelerated Bone Defect Repair in Rats by Targeting Ferroptosis Pathway DOI Creative Commons
Xinhua Xu, Junming Feng,

Tsung-Chieh Lin

et al.

Journal of Functional Biomaterials, Journal Year: 2024, Volume and Issue: 15(12), P. 385 - 385

Published: Dec. 20, 2024

Background: The reparative regeneration of jawbone defects poses a significant challenge within the field dentistry. Despite being gold standard, autogenous bone materials are not without drawbacks, including heightened risk postoperative infections. Consequently, development innovative that can surpass osteogenic capabilities autologous has emerged as pivotal area research. Methods: Mesenchymal stem cells (MSCs), known for their multilineage differentiation potential, were isolated from human umbilical cords and transfected with miR-181a. miR-181a/MSC was investigated. Then, physicochemical properties miR-181a/MSC-loaded nano-hydroxyapatite (nHAC) scaffolds characterized, efficacy underlying mechanism in rat calvarial defect repair explored. Results: miR-181a overexpression MSCs significantly promoted differentiation, evidenced by increased alkaline phosphatase activity expression markers. nHAC exhibited favorable bioactivity accelerated tissue collagen secretion vivo. Mechanistic studies reveal directly targeted TP53/SLC7A11 pathway, inhibiting ferroptosis enhancing capacity MSCs. Conclusions: study demonstrates enhance promoting ferroptosis. These findings provide novel insights into molecular mechanisms regulating MSC osteogenesis offer promising therapeutic strategy repair.

Language: Английский

Tea-break with epigallocatechin gallate derivatives – Powerful polyphenols of great potential for medicine DOI Creative Commons
Paweł Bakun, Dariusz T. Mlynarczyk, Tomasz Koczorowski

et al.

European Journal of Medicinal Chemistry, Journal Year: 2023, Volume and Issue: 261, P. 115820 - 115820

Published: Sept. 14, 2023

Epigallocatechin gallate (EGCG) is a polyphenol present in green tea (Camellia sinensis), which has revealed anti-cancer effects toward variety of cancer cells vitro and protective potential against neurodegenerative diseases such as Alzheimer's Parkinson's. Unfortunately, EGCG presents disappointing bioavailability after oral administration, primarily due to its chemical instability poor absorption. Due these limitations, currently not used medication, but only dietary supplement the form extract. Therefore, it needs further modifications before being considered suitable for extensive medical applications. In this article, we review scientific literature about derivatives focusing on their biological properties The most common epigallocatechin rely introducing fatty acid chains or sugar molecules structure modify solubility. Another frequently employed procedure based blocking EGCG's hydroxyl groups with various substituents. Novel reveal interesting properties, which, antioxidant, anti-inflammatory, antitumor antimicrobial, are especially important. It worth noting that promising higher stability activity than base EGCG.

Language: Английский

Citations

32
Liver fibrosis as a barometer of systemic health by gauging the risk of extrahepatic disease DOI Open Access
Amedeo Lonardo, Stefano Ballestri, György Baffy

et al.

Metabolism and Target Organ Damage, Journal Year: 2024, Volume and Issue: 4(4)

Published: Nov. 15, 2024

This review article proposes the theory that liver fibrosis, abnormal accumulation of excessive extracellular matrix, is not just an indicator disease but also a negative reflection overall systemic health. Liver fibrosis poses heavy financial burden on healthcare systems worldwide and can develop due to chronic from various causes, often sustained inflammation. may generate symptoms become apparent only when it reaches stage cirrhosis associated with clinically significant portal hypertension leads decompensation events or promotes development hepatocellular carcinoma. While viral hepatitis alcohol consumption were once primary causes featuring this role now increasingly taken over by metabolic dysfunction-associated steatotic (MASLD). In MASLD, endothelial dysfunction essential component in pathogenesis, promoting present cells other organs such as heart, lungs, kidneys. Accordingly, predictor liver-related outcomes, well all-cause mortality, cardiovascular risk, extrahepatic cancer. Physicians should be aware individuals seeking medical attention for reasons unrelated health have advanced fibrosis. Early identification these at-risk lead more comprehensive assessment use treatment options, both approved investigational, slow reverse progression

Language: Английский

Citations

5
Sex-Specific Biochemical and Histopathological Effects of Chronic Meat-Based vs. Plant-Based Burger Consumption in a Rodent Model DOI Creative Commons
Cristina Filip, Ruxandra Ștefănescu, Bianca-Eugenia Ősz

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(5), P. 888 - 888

Published: March 5, 2025

The growing consumption of processed foods, including meat-based and plant-based burgers (PBM), raises concerns about their long-term health effects. While PBM are marketed as healthier alternatives, biochemical histological impacts remain unclear. This study investigates the effects chronic burger on metabolic organ health, considering sex differences. Thirty-six Wistar rats (18 males 18 females) were divided into three groups: control (standard chow), meat burger-fed, burger-fed. Improved chow was prepared using lyophilized powder. Over eight weeks, food intake, weight gain, efficiency ratio (FER) monitored. Serum markers, AST, ALT, urea, creatinine, lipid profile (TG, CHOL, HDL, LDL), leptin, analyzed, alongside histopathological evaluation liver, kidneys, heart. PBM-fed exhibited significantly higher FER than group (p < 0.05). AST ALT levels slightly elevated in meat-fed rats, while had increased urea levels. Triglyceride group, but cholesterol did not differ significantly. leptin both experimental groups, suggesting resistance. Histological findings showed mild hepatic inflammation microvacuolar changes, with minor cardiac fibrosis. These highlight need for further research PBM's

Language: Английский

Citations

0
Nanoparticle-based therapeutic strategies for chronic liver diseases: Advances and insights DOI Creative Commons

Padmanaban Sathiyamoorthy,

Jiwon Baek,

Sai Sahithya Chamarthy

et al.

Liver Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Growth differentiation factor 7 alleviates the proliferation and metastasis of hepatocellular carcinoma DOI Creative Commons

Jianyong Zhuo,

Huigang Li, Peiru Zhang

et al.

Liver Research, Journal Year: 2024, Volume and Issue: 8(4), P. 259 - 268

Published: Oct. 10, 2024

Inflammatory factors play significant roles in the development and occurrence of hepatocellular carcinoma (HCC). However, tumor-protective functions growth differentiation (GDFs) HCC are yet to be clarified. In this study, we aimed evaluate expression levels 10 GDFs tumor paratumor tissues from patients with perform vitro vivo experiments elucidate role GDF7 regulating proliferation metastasis HCC. The gene was compared between paratumors using Cancer Genome Atlas dataset patient-derived tissues. A microarray containing 108 tissue samples used explore prognostic value expression. Loss-of-function were also performed investigate mRNA protein significantly lower tumors than (P < 0.001). Kaplan-Meier analysis showed that decreased associated worse overall survival (5-year rate: 61.8% vs. 27.5%, P 0.001) increased recurrence risk Multivariate Cox regression demonstrated low expression, presence microvascular invasion, elevated alpha-fetoprotein (AFP) independent for poor survival. Downregulation cells an xenograft model. knockdown promoted migration invasion via epithelial-mesenchymal transition. Meanwhile, a negative correlation JunB proto-oncogene (JUNB) observed Modulating JUNB altered is potential biomarker predicting superior outcomes amplification therapeutic option

Language: Английский

Citations

0
Current status and new directions for hepatocellular carcinoma diagnosis DOI Creative Commons

Jinqi Tu,

Bo Wang,

Xiaoming Wang

et al.

Liver Research, Journal Year: 2024, Volume and Issue: 8(4), P. 218 - 236

Published: Dec. 1, 2024

Liver cancer ranks as the sixth most common globally, with hepatocellular carcinoma (HCC) accounting for approximately 75%-85% of cases. Most patients present moderately advanced disease, while those HCC face limited and ineffective treatment options. Despite diagnostic efforts, no ideal tumor marker exists to date, highlighting urgent clinical need improved early detection HCC. A key research objective is development assays that target specific pathways involved in progression. This review explores pathological origin HCC, providing insights into mechanistic rationale, statistics, advantages limitations commonly used markers. Additionally, it discusses potential emerging biomarkers diagnosis offers a brief overview relevant assay methodologies. aims summarize existing markers investigate new ones, basis subsequent research.

Language: Английский

Citations

0
miR-181a/MSC-Loaded Nano-Hydroxyapatite/Collagen Accelerated Bone Defect Repair in Rats by Targeting Ferroptosis Pathway DOI Creative Commons
Xinhua Xu, Junming Feng,

Tsung-Chieh Lin

et al.

Journal of Functional Biomaterials, Journal Year: 2024, Volume and Issue: 15(12), P. 385 - 385

Published: Dec. 20, 2024

Background: The reparative regeneration of jawbone defects poses a significant challenge within the field dentistry. Despite being gold standard, autogenous bone materials are not without drawbacks, including heightened risk postoperative infections. Consequently, development innovative that can surpass osteogenic capabilities autologous has emerged as pivotal area research. Methods: Mesenchymal stem cells (MSCs), known for their multilineage differentiation potential, were isolated from human umbilical cords and transfected with miR-181a. miR-181a/MSC was investigated. Then, physicochemical properties miR-181a/MSC-loaded nano-hydroxyapatite (nHAC) scaffolds characterized, efficacy underlying mechanism in rat calvarial defect repair explored. Results: miR-181a overexpression MSCs significantly promoted differentiation, evidenced by increased alkaline phosphatase activity expression markers. nHAC exhibited favorable bioactivity accelerated tissue collagen secretion vivo. Mechanistic studies reveal directly targeted TP53/SLC7A11 pathway, inhibiting ferroptosis enhancing capacity MSCs. Conclusions: study demonstrates enhance promoting ferroptosis. These findings provide novel insights into molecular mechanisms regulating MSC osteogenesis offer promising therapeutic strategy repair.

Language: Английский

Citations

0