β-Nicotinamide Mononucleotide Reduces Oxidative Stress and Improves Steroidogenesis in Granulosa Cells Associated with Sheep Prolificacy via Activating AMPK Pathway DOI Creative Commons
Yu Cai, Hua Yang, Hui Xu

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 14(1), P. 34 - 34

Published: Dec. 30, 2024

Oxidative stress is a significant factor in the death of granulosa cells (GCs), leading to follicular atresia and consequently limiting number dominant follicles that can mature ovulate within each wave. Follicular fluid contains diverse array metabolites play crucial roles regulating GCs’ proliferation oocyte maturation, which are essential for follicle development female fertility. However, mechanisms behind metabolite heterogeneity its effects on function remain poorly understood. Here, we identified elevated nicotinamide levels high-prolificacy sheep, correlated with oxidative GCs, by an integrated analysis. In vitro experiments demonstrated supplementation β-nicotinamide mononucleotide (NMN) significantly increased adenine dinucleotide (NAD+) adenosine triphosphate (ATP) GCs. NMN treatment effectively reduced Lipopolysaccharide (LPS)-induced apoptosis mitigated mitochondrial dysfunction, while also decreasing production reactive oxygen species (ROS), thereby enhancing activity antioxidant defense system. Importantly, improved impairments steroid hormone induced LPS. Mechanistically, protective against GCs were mediated via AMPK/mTOR pathway. Collectively, our findings elucidate metabolic characteristics associated sheep prolificacy demonstrate protects from LPS-induced dysfunction enhances ovarian responsiveness These position as potential novel biomarker function.

Language: Английский

Sex-specific mechanisms in vascular aging: exploring cellular and molecular pathways in the pathogenesis of age-related cardiovascular and cerebrovascular diseases DOI Creative Commons
Anna Ungvari, Rafał Gulej, Roland Patai

et al.

GeroScience, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 3, 2025

Language: Английский

Citations

3
Use of Nicotinamide Mononucleotide as Non-Natural Cofactor DOI Open Access
Tahseena Naaz, Beom Soo Kim

Catalysts, Journal Year: 2025, Volume and Issue: 15(1), P. 37 - 37

Published: Jan. 3, 2025

Nicotinamide mononucleotide (NMN) has emerged as a promising non-natural cofactor with significant potential to transform biocatalysis, synthetic biology, and therapeutic applications. By modulating NAD⁺ metabolism, NMN offers unique advantages in enzymatic reactions, metabolic engineering, regenerative medicine. This review provides comprehensive analysis of NMN’s biochemical properties, mechanisms action, diverse Emphasis is placed on its role addressing challenges multi-enzyme cascades, biofuel production, the synthesis high-value chemicals. The paper also highlights critical research gaps, including need for scalable methods, improved integration into systems, toxicity studies use. Emerging technologies such AI-driven enzyme design CRISPR-based genome engineering are discussed transformative tools optimizing NMN-dependent pathways. Furthermore, synergistic biology innovations, cell-free systems dynamic regulatory networks, explored, paving way precise modular biotechnological solutions. Looking forward, versatility positions it pivotal tool advancing sustainable bioprocessing precision Addressing current limitations through interdisciplinary approaches will enable redefine boundaries innovation. serves roadmap leveraging across scientific industrial domains.

Language: Английский

Citations

0
The Effect of Nicotinamide Mononucleotide and Riboside on Skeletal Muscle Mass and Function: A Systematic Review and Meta‐Analysis DOI Creative Commons
Konstantinos P. Prokopidis, Frank Moriarty, Gülistan Bahat

et al.

Journal of Cachexia Sarcopenia and Muscle, Journal Year: 2025, Volume and Issue: 16(3)

Published: April 24, 2025

Language: Английский

Citations

0
Nicotinamide Mononucleotide Supplementation: Understanding Metabolic Variability and Clinical Implications DOI Creative Commons
Candace Benjamin,

Rebecca Crews

Metabolites, Journal Year: 2024, Volume and Issue: 14(6), P. 341 - 341

Published: June 18, 2024

Recent years have seen a surge in research focused on NAD+ decline and potential interventions, despite significant progress, new discoveries continue to highlight the complexity of biology. Nicotinamide mononucleotide (NMN), well-established precursor, has garnered considerable interest due its capacity elevate levels induce promising health benefits preclinical models. Clinical trials investigating NMN supplementation yielded variable outcomes while shedding light intricacies metabolism revealing critical roles played by gut microbiota specific cellular uptake pathways. Individual variability factors such as lifestyle, conditions, genetics, microbiome composition likely contributes observed discrepancies clinical trial results. Preliminary evidence suggests that NMN’s effects may be context-dependent, varying based person’s physiological state. Understanding these nuances is for definitively assessing impact manipulating through supplementation. Here, we review metabolism, focusing current knowledge, pinpointing key areas where further needed, outlining future directions advance our understanding significance.

Language: Английский

Citations

3
Β-Nicotinamide Mononucleotide Attenuates Oxidative Stress and Activates Steroidogenesis in Sheep Ovarian Granulosa Cells by Ampk/Ulk1/Mtor Pathway DOI
Yu Cai, Hua Yang, Hui Xu

et al.

Published: Jan. 1, 2024

Follicular granulosa cells (GCs) are responsive in the increase of ovulation number, thereby indirectly influencing litter size. Oxidative stress-induced GCs death represents a common reason for follicular atresia, although underlying metabolic molecules remains to be elucidated. Here, we provided first combined metabolomic fluid and cellular transcriptomic analysis sheep associated with high low prolificacy. Then explored effects β-Nicotinamide mononucleotide (NMN) on biological behaviors lipopolysaccharide (LPS)-induced potential mechanisms. Our results showed that NMN increased levels Nicotinamide Adenine Dinucleotide adenosine triphosphate (NAD+ ATP), suppressed LPS-induced cell apoptosis as indicated by enhancing expression BAX PARP1, well alleviated mitochondrial dysfunction through genes oxidative phosphorylation (Naufa1 Ndufs6). Moreover, attenuated stress inhibiting production reactive oxygen species (ROS), lipid peroxidation marker (MDA), promoted activity antioxidative system including CAT, GSH, T-AOC, SOD. Mechanically, found exposure LPS stimulated improved impaired estrogen (E2) progesterone (P4) along abundances steroidogenic enzyme mRNA transcripts, STAR, CYP19A1, 3β-HSD, then altered AMPK, mTOR, ULK1. Further, might play crucial role primary follicle formation ovary cortex cultures at tissue level. Findings indicate beneficial effect is mediated eliminating accumulated ROS suppress restoring function via AMPK/ULK1/mTOR signaling pathway.

Language: Английский

Citations

0
Efficacy of oral nicotinamide mononucleotide supplementation on glucose and lipid metabolism for adults: a systematic review with meta-analysis on randomized controlled trials DOI Creative Commons
Jiaqi Zhang, Eric Tsz‐Chun Poon, Stephen Heung‐Sang Wong

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 19

Published: Aug. 8, 2024

A surge of public interest in NMN supplementation has been observed recent years. However, whether supplements are effective improving metabolic health remains unclear. The objective the review was to assess effects on fasting glucose, triglycerides, total cholesterol, LDL-C, and HDL-C adults. Studies were located by searching four databases (PubMed, Embase, Cochrane, Web Science). Two reviewers independently conducted title/abstract full-text screening, data extraction, risk-of-bias assessment. Of 4049 records reviewed, 12 studies with a 513 participants met criteria for analysis. Random-effects meta-analyses found an overall significant effect elevating blood NAD levels. most clinically relevant outcomes not significantly different between control group. Risk-of-bias assessment using RoB2 showed some concerns seven high risk bias other five studies. Together, our findings suggest that exaggeration benefits may exist field. Although limited number eligible sufficiently powered detect changes abovementioned primary outcomes, more needed conclude about exact supplementation.

Language: Английский

Citations

0
Exploring the Therapeutic Potential of Nicotinamide Mononucleotide: A Review of Safety and Efficacy with Emphasis on Diabetes DOI Creative Commons
Hubert Gugulski, Michał Andrzej Kozicz,

Szymon Rudawski

et al.

Quality in Sport, Journal Year: 2024, Volume and Issue: 17, P. 53437 - 53437

Published: Aug. 13, 2024

Introduction: Nicotinamide mononucleotide (NMN) is a supplement that can boost nicotinamide adenine dinucleotide (NAD+) levels in the body, which crucial for maintaining energy balance and stress response. Declining NAD+ with age contribute to aging related diseases, making NMN supplementation potential solution. Despite its benefits, NMN’s safety efficacy need further investigation. Aim of Study: This study aimed analyze recent research on regarding improving human health, especially patients diabetes prediabetes, suggest directions this compound. Methods Materials: Extensive was conducted using PubMed Google Scholar, focusing literature from past 5 years, primarily randomized controlled trials. The databases were searched term “nicotinamide mononucleotide” gather articles, references selected articles included. Conclusion: A beneficial direction an in-depth analysis effects skeletal muscles both healthy individuals those diabetes. Studies larger groups, higher doses, over longer periods are determine optimal protocol assess individual variability metabolic effects.

Language: Английский

Citations

0
β-Nicotinamide Mononucleotide Reduces Oxidative Stress and Improves Steroidogenesis in Granulosa Cells Associated with Sheep Prolificacy via Activating AMPK Pathway DOI Creative Commons
Yu Cai, Hua Yang, Hui Xu

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 14(1), P. 34 - 34

Published: Dec. 30, 2024

Oxidative stress is a significant factor in the death of granulosa cells (GCs), leading to follicular atresia and consequently limiting number dominant follicles that can mature ovulate within each wave. Follicular fluid contains diverse array metabolites play crucial roles regulating GCs’ proliferation oocyte maturation, which are essential for follicle development female fertility. However, mechanisms behind metabolite heterogeneity its effects on function remain poorly understood. Here, we identified elevated nicotinamide levels high-prolificacy sheep, correlated with oxidative GCs, by an integrated analysis. In vitro experiments demonstrated supplementation β-nicotinamide mononucleotide (NMN) significantly increased adenine dinucleotide (NAD+) adenosine triphosphate (ATP) GCs. NMN treatment effectively reduced Lipopolysaccharide (LPS)-induced apoptosis mitigated mitochondrial dysfunction, while also decreasing production reactive oxygen species (ROS), thereby enhancing activity antioxidant defense system. Importantly, improved impairments steroid hormone induced LPS. Mechanistically, protective against GCs were mediated via AMPK/mTOR pathway. Collectively, our findings elucidate metabolic characteristics associated sheep prolificacy demonstrate protects from LPS-induced dysfunction enhances ovarian responsiveness These position as potential novel biomarker function.

Language: Английский

Citations

0