Journal of Clinical Medicine,
Journal Year:
2025,
Volume and Issue:
14(6), P. 1856 - 1856
Published: March 10, 2025
The
extracellular
matrix
(ECM)
is
the
common
denominator
of
more
than
50
chronic
diseases.
Some
these
pathologies
lead
to
enhanced
tissue
formation
and
deposition,
whereas
others
are
associated
with
increased
degradation,
some
exhibit
a
combination
both,
leading
severe
alterations.
To
develop
effective
therapies
for
diseases
affecting
lung,
liver,
kidney,
skin,
intestine,
musculoskeletal
system,
heart,
solid
tumors,
we
need
modulate
ECM’s
composition
restore
its
organization
function.
Across
diverse
organ
diseases,
there
denominators
distinguishing
factors
in
this
fibroinflammatory
axis,
which
may
be
used
foster
new
insights
into
drug
development
across
disease
indications.
2nd
Extracellular
Matrix
Pharmacology
Congress
took
place
Copenhagen,
Denmark,
from
17
19
June
2024
was
hosted
by
International
Society
Pharmacology.
event
attended
450
participants
35
countries,
among
whom
were
prominent
scientists
who
brought
together
state-of-the-art
research
on
asked
important
questions
facilitate
development.
We
highlight
key
aspects
ECM
lungs,
tumors
advance
our
understanding
central
targets
also
advances
tools
technology
that
enable
development,
thereby
supporting
ECM.
Journal of Biological Engineering,
Journal Year:
2024,
Volume and Issue:
18(1)
Published: Feb. 22, 2024
Abstract
Background
The
extracellular
matrix
(ECM)
is
a
three-dimensional
network
of
proteins
that
encases
and
supports
cells
within
tissue
promotes
physiological
pathological
cellular
differentiation
functionality.
Understanding
the
complex
composition
ECM
essential
to
decrypt
processes
as
well
pathogenesis.
In
this
context,
method
decellularization
useful
technique
eliminate
components
from
tissues
while
preserving
majority
structural
functional
integrity
ECM.
Results
study,
we
employed
bottom-up
proteomic
approach
elucidate
intricate
in
decellularized
matrices
murine
liver
kidney
tissues.
This
involved
use
novel,
perfusion-based
protocol
generate
acellular
whole
organ
scaffolds.
Proteomic
analysis
mice
scaffolds
revealed
tissue-specific
differences
matrisome
composition,
found
predominantly
stable
core
matrisome,
consisting
collagens,
glycoproteins,
proteoglycans.
Liver
unique
such
collagen
type
VI
alpha-6,
fibrillin-2
or
biglycan.
kidney,
specific
ECM-regulators
cathepsin
z
were
detected.
Conclusion
identification
distinct
signatures
provides
insights
into
how
different
compositions
might
influence
biological
properties
experimental
workflow
will
help
further
landscape
order
decipher
cell–matrix
interactions
their
contribution
microenvironment.
FEBS Letters,
Journal Year:
2024,
Volume and Issue:
598(6), P. 602 - 620
Published: March 1, 2024
The
extracellular
matrix
(ECM)
proteome
represents
an
important
component
of
the
tissue
microenvironment
that
controls
chemical
flux
and
induces
cell
signaling
through
encoded
structure.
analysis
ECM
analytical
challenge
high
levels
post‐translational
modifications,
protease‐resistant
structures,
crosslinked,
insoluble
proteins.
This
review
provides
a
comprehensive
overview
challenges
involved
in
addressing
complexities
spatially
profiling
proteome.
A
synopsis
process
synthesizing
structure,
detailing
inherent
complexity,
is
included
to
present
scope
challenge.
Current
chromatographic
spatial
techniques
these
are
detailed.
Capabilities
for
multimodal
multiplexing
with
cellular
populations
discussed
perspective
on
developing
holistic
view
disease
processes
includes
both
microenvironment.
Matrix Biology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Rapid
progress
has
been
made
in
the
exciting
field
of
secretome
research
health
and
disease.
The
tumor
secretome,
which
is
a
significant
proportion
proteome,
secreted
into
extracellular
space
to
promote
intercellular
communication
thus
progression.
Among
many
molecules
integrins
matrix
metalloproteinase
14
(MMP14)
stand
out
as
interplay
adhesion
proteolysis
drives
invasion.
Integrins
serve
mechanosensors
that
mediate
contact
cells
with
scaffold
are
significantly
involved
precise
positioning
activity
control
membrane-bound
collagenase
MMP14.
As
proteinase,
MMP14
influences
modifies
itself.
While
MT-MMPs
membrane
bound,
but
can
be
released
therefore
border
crossers
between
cell
surface
not
constitutively
cell-bound,
its
binding
other
receptors
stringently
regulated
process.
To
understand
mutual
interactions
detail,
we
first
summarize
structure
function
how
it
at
enzymatic
cellular
level.
In
particular,
include
proteolytic
cleavage
themselves
by
We
then
review
biochemical,
biological
physiological
effects
on
composition
associated
functions
when
either
bound
membrane,
or
located
microvesicles,
proteolytically
shed
non-membrane-bound
ectodomain.
Novel
methods
proteomics,
including
analysis
extravesicular
vesicles,
new
for
quantification
will
provide
diagnostic
tools.
modification
especially
MMP14,
may
bring
an
additional
aspect
studies
have
impact
diagnosis
most
likely
also
therapy
cancer
patients.
BMC Cancer,
Journal Year:
2025,
Volume and Issue:
25(1)
Published: Feb. 12, 2025
Collagen
type
X
(ColXα1,
encoded
by
COL10A1)
is
expressed
specifically
in
the
cartilage-to-bone
transition,
bone
marrow
cells,
and
osteoarthritic
(OA)
cartilage.
We
have
previously
shown
that
ColXα1
breast
tumor
stroma,
correlates
with
tumor-infiltrating
lymphocytes,
predicts
poor
adjuvant
therapy
outcomes
ER+/HER2+
cancer.
However,
underlying
molecular
mechanisms
for
these
effects
are
unknown.
In
this
study,
we
performed
bioinformatic
analysis
of
COL10A1-associated
gene
modules
pancreatic
cancer
as
well
cells
from
OA
These
findings
provide
important
insights
into
transcriptional
extracellular
matrix
changes
which
impact
local
stromal
microenvironment
progression.
Immunohistochemistry
was
to
examine
collagen
expression
solid
tumors.
WGCNA
used
generate
networks
cohorts
using
RNA-Seq
data
The
Cancer
Genome
Atlas.
Computational
employed
assess
on
development
progression
OA.
Data
processing
statistical
R
various
publicly-available
computational
tools.
Expression
COL10A1
its
associated
highlights
inflammatory
immunosuppressive
microenvironments,
identify
aggressive
tumors
contribute
metastatic
potential
a
sex-dependent
manner.
Both
types
enriched
implicates
marrow-derived
fibroblasts
contributors
epithelial-to-mesenchymal
transition
(EMT)
Heightened
correlated
poorer
patient
both
Common
chondrogenic
activity
shared
between
cartilage,
suggesting
similar
microenvironmental
alterations
may
underlie
diseases.
hold
substantial
value
regulators
biomarkers
phenotypes
implications
clinical
outcomes.
Identification
exhibit
high
genes
reveal
presence
microenvironments
heightened
EMT
capacity
potential.
Our
enable
more
effective
risk
assessment
precise
treatment
patients
Journal of Cell Science,
Journal Year:
2025,
Volume and Issue:
138(6)
Published: March 15, 2025
ABSTRACT
The
organization
and
mechanics
of
extracellular
matrix
(ECM)
protein
polymers
determine
tissue
structure
function.
Secreted
ECM
components
are
assembled
into
via
a
cell-mediated
process.
specific
mechanisms
that
cells
use
for
assembly
crucial
generating
tissue-appropriate
matrices.
Fibronectin
(FN)
is
ubiquitous
abundant
fibrillar
by
receptor-mediated
process,
the
FN
provides
foundation
incorporation
many
other
proteins
ECM.
In
this
Cell
Science
at
Glance
article
accompanying
poster,
we
describe
domain
events
initiate
propagate
stable
insoluble
network
fibrils.
We
also
discuss
intracellular
pathways
regulate
impact
changes
in
on
disease
progression.
