Human TMPRSS2 and ACE2 Genetic Variability on COVID-19 Outcomes in Patients From Brazil DOI Open Access
Felipe Pantoja Mesquita, Jean Breno Silveira da Silva, Laís Lacerda Brasil de Oliveira

et al.

Published: Oct. 25, 2023

Angiotensin-converting enzyme 2 (ACE2) and the transmembrane serine protease (TMPRSS2) are recognized as entry proteins of severe acute respiratory syndrome coronavirus (SARS-CoV-2), recently their Single Nucleotide Polymorphisms (SNP) have been studied in different populations to elucidate impact on disease. The aim this study was evaluate genetic SNP ACE2 (rs35803318) TMPRSS2 (rs2070788) genes COVID-19 patients from Northeast Brazil compared with global populations, well expression quantitative trait locus (eQTL). For (rs35803318), we found 92.6% CC, 3.4% CT, 4.0% TT genotype carriers SARS-CoV-2-positive patients. Surprisingly, only frequencies were not Hardy-Weinberg equilibrium. rs2070788, 22.3% GG, 50.7% AG, 27% AA loci (eQTLs) revealed that rs35803318 associated an altered PIR gene expression, rs2070788 eQTLs association lung tissue. No significant identified between distribution SNPs 'patient's outcome. In conclusion, our results suggest may be protective factors for including Brazilian population, since presence does affect outcome described by other studies.

Language: Английский

The immunogenetics of COVID-19 DOI Open Access
Anshika Srivastava,

Jill A. Hollenbach

Immunogenetics, Journal Year: 2022, Volume and Issue: 75(3), P. 309 - 320

Published: Dec. 19, 2022

Language: Английский

Citations

14
Novel genetic association of the Furin gene polymorphism rs1981458 with COVID-19 severity among Indian populations DOI Creative Commons
Rudra Kumar Pandey, Anshika Srivastava, Rahul Mishra

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 3, 2024

Abstract SARS CoV-2, the causative agent for ongoing COVID-19 pandemic, it enters host cell by activating ACE2 receptor with help of two proteasesi.e., Furin and TMPRSS2. Therefore, variations in these genes may account differential susceptibility severity between populations. Previous studies have shown that role TMPRSS2 gene variants understanding among Indian Nevertheless, a knowledge gap exists concerning diverse South Asian ethnic groups. Investigating their global phylogeographic structure is essential to comprehensively We used 450 samples from states performed linear regression analyse variant's Case Fatality Rate (CFR) could be epidemiologically associated disease outcomes. Associated genetic were further evaluated expression regulatory potential through various Insilco analyses. Additionally, we examined using next-generation sequencing (NGS) data 393 samples, particular emphasis on Asia, investigate its Phylogeographic world found significant positive association SNP rs1981458 CFR (p < 0.05) populations at different timelines first second waves. Further, QTL other analyses showed associations roles gene, mainly Immune cells virus infection process, highlighting immunity viral assembly processing. The protein–protein interaction suggested contribute Pulmonary arterial hypertension via typical inflammation mechanism. architecture demonstrated closer affinity Asia West Eurasian worth proposing Asians will more similar population. Our previous East Eurasians Eurasians, respectively. collective information three important (ACE2, Furin) modelled susceptibilityof major ancestries an inclination towards Eurasia. In conclusion, this study, time, concluded population outlined potential.This study also highlights distinct, however, inclined believe insight utilised as biomarker identify vulnerable populations, which might directly relevant developing policies allocating resources effectively during epidemic.

Language: Английский

Citations

2
Influence of polymorphic variations of IFNL, HLA, and IL-6 genes in severe cases of COVID-19 DOI
Adrhyan Araújo, Gabriella Sgorlon, Letícia Ereira Aguiar

et al.

Experimental Biology and Medicine, Journal Year: 2023, Volume and Issue: 248(9), P. 787 - 797

Published: May 1, 2023

The administration of vaccination doses to the global population has led a decrease in incidence COVID-19. However, clinical picture developed by infected individuals remains extremely concerning due great variability severity cases even vaccinated individuals. progression pathology is characterized various influential factors such as sex, age group, comorbidities, and genetics individual. immune response viral infections can be strongly influenced individuals; nucleotide variations called single-nucleotide polymorphisms (SNPs) structures involved innate adaptive interferon (IFN)-λ, human leukocyte antigen (HLA), interleukin (IL)-6 are frequently associated with pathological progression. In this study, we conducted review main SNPs these that Searches were on some platforms National Center for Biotechnology Information (NCBI), 102 studies selected full reading according inclusion criteria. IFNs showed strong association antiviral function, specifically, IFN-λ3 (IL-28B) demonstrated genetic variants commonly related pathologies. For COVID-19, rs12979860 rs1298275 presented described unfavorable genotypes conditions hepatitis C hepatocellular carcinoma. high HLA was reported crucial factor relevant late response, mainly its ability recognize antigens, HLA-B*46:01 SNP being susceptibility IL-6, rs1554606 relationship addition, rs2069837 identified possible host protection relationships when linked infection.

Language: Английский

Citations

6
The fatal contribution of serine protease-related genetic variants to COVID-19 outcomes DOI Creative Commons
Laura E. Martínez-Gómez, Carlos Martínez-Armenta,

Teresa Tusié‐Luna

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: March 27, 2024

Serine proteases play a critical role during SARS-CoV-2 infection. Therefore, polymorphisms of transmembrane protease serine 2 (TMPRSS2) and serpine family E member 1 (SERPINE1) could help to elucidate the contribution variability COVID-19 outcomes. To evaluate genetic variants genes previously associated with outcomes, we performed cross-sectional study in which 1536 SARS-CoV-2-positive participants were enrolled. TMPRSS2 (rs2070788, rs75603675, rs12329760) SERPINE1 (rs2227631, rs2227667, rs2070682, rs2227692) genotyped using Open Array Platform. The association disease outcomes was determined by logistic regression analysis adjusted for covariates (age, sex, hypertension, type diabetes, obesity). According our codominant model, GA genotype rs2227667 (OR=0.55; 95% CI = 0.36-0.84; p=0.006) AG (OR=0.59; 0.38-0.91; p=0.02) played protective against disease. However, rs2227692 T allele TT (OR=1.45; 1.11-1.91; p=0.006; OR=2.08; 1.22-3.57; p=0.007; respectively) decreased risk death. Similarly, rs75603675 AA had an OR 1.97 (95% 1.07-3.6; p=0.03) deceased patients. Finally, increased D-dimer levels (OR=1.24; 1.03-1.48; p=0.02). Our data suggest that are poor outcome. Additionally, participate hypercoagulable conditions patients, this variant contribute identification new pharmacological targets treatment strategies block inhibition entry into SARS-CoV-2.

Language: Английский

Citations

2
Association of ACE2 and TMPRSS2 genes variants with disease severity and most important biomarkers in COVID-19 patients in Bosnia and Herzegovina DOI Creative Commons
Neven Meseldžić, Besim Prnjavorac, Tanja Dujić

et al.

Croatian Medical Journal, Journal Year: 2024, Volume and Issue: 65(3), P. 220 - 232

Published: June 1, 2024

To assess the association of single nucleotide polymorphisms (SNPs) in ACE2 and TMPRSS2 genes with COVID-19 severity key biomarkers.

Language: Английский

Citations

1
Human TMPRSS2 and ACE2 genetic variability on COVID-19 outcomes in patients from Brazil DOI
Felipe Pantoja Mesquita, Jean Breno Silveira da Silva, Laís Lacerda Brasil de Oliveira

et al.

Human Gene, Journal Year: 2024, Volume and Issue: 41, P. 201310 - 201310

Published: June 26, 2024

Language: Английский

Citations

1
Role of ACE2 and TMPRSS2 polymorphisms in clinical severity and outcomes of COVID-19 in Egypt DOI Creative Commons
Walaa Samy, O. Gaber, Rania Amer

et al.

African Journal of Laboratory Medicine, Journal Year: 2024, Volume and Issue: 13(1)

Published: Aug. 27, 2024

The clinical presentations of coronavirus disease 2019 (COVID-19) exhibit significant variation, ranging from asymptomatic cases to mortality resulting severe pneumonia. Host genetics can partially explain this variation.

Language: Английский

Citations

1
Transmembrane Serine Protease-2 Gene Polymorphism and Expression in Iraqi COVID-19 Patients DOI Creative Commons
Nawal Mohammed Utba

JOURNAL OF COMMUNICABLE DISEASES, Journal Year: 2023, Volume and Issue: 55(03), P. 75 - 82

Published: Dec. 7, 2023

Introduction: The global COVID-19 pandemic was caused by SARSCoV-2. Human cells ingest this virus when ACE2 identifies it. TMPRSS2 prepares SARS-CoV-2 for entry. clinical outcomes of are associated with and gene expression polymorphisms.Objective: To determine if the rs 2070788 SNP in intron 11–12 is severe Iraqi patients.Methods: study included 120 patients from three Ramadi City hospitals 80 healthy controls. DNA extraction done Wizard genomic TM Extraction Kit, RNA One Script Plus cDNA Synthesis qRT-PCR used investigating genetic polymorphism.Results: rs2070788 had genotypes (CC, CT, TT) two alleles. controls were compatible Hardy-Weinberg equilibrium (HWE). Severe significantly higher TT genotype frequencies (28% vs 4%, OR = 9.33; 95% CI 2.03 to 43.01; p 0.002) T allele (48% 16%, 2.37; 1.32 4.26; 0.005), respectively. mRNA much lower cases than In addition, relative increased 1.15 ± 0.71 folds CC compared CT (0.632 0.25) (0.552 0.193) genotypes, but difference not significant (p > 0.05).Conclusion: Iraqis highly susceptible due allele. also downregulated expression. How cite article:Abed TA, Utba NM, Kareem AHA. Transmembrane Serine Protease-2 Gene Polymorphism Expression Patients. J Commun Dis. 2023;55(3):75-82. DOI: https://doi.org/10.24321/0019.5138.202342

Language: Английский

Citations

3
TMPRSS2 Impacts Cytokine Expression in Murine Dendritic Cells DOI Creative Commons
Sandra Gunne,

Marie Schwerdtner,

Marina Henke

et al.

Biomedicines, Journal Year: 2023, Volume and Issue: 11(2), P. 419 - 419

Published: Feb. 1, 2023

The transmembrane protease serine 2 (TMPRSS2) proteolytically activates the envelope proteins of several viruses for viral entry via membrane fusion and is therefore an interesting promising target development broad-spectrum antivirals. However, use a host protein as may lead to potential side effects, especially on immune system. We examined effect genetic deletion TMPRSS2 dendritic cells.Bone marrow cells from wild-type (WT) TMPRSS2-deficient mice (TMPRSS2-/-) were differentiated plasmacytoid (pDCs) classical DCs (cDCs) activated with various toll-like receptor (TLR) agonists. analyzed released cytokines mRNA expression chemokine receptors, TLR7, TLR9, IRF7 TCF4 stimulation.In cDCs, lack led increase in IL12 IFNγ TLR7/8 agonist resiquimod or TLR 9 ODN 1668-activated cells. Only IL-10 was reduced TMPRSS2-/- comparison WT 1668. In resiquimod-activated pDCs, decrease IL-6, INFγ. 1668 activation reduction IFNα. pDCs cDCs low.The pDCS depends TLR, seems affect cytokine release differently cDCs. suppress release, whereas possibly mediates release.

Language: Английский

Citations

2
Genetic Susceptibility to Severe Forms of COVID-19: What we learned in 2022 DOI Open Access
R. Benmansour,

M. R. Tagajdid,

S. Elkochri

et al.

Saudi Journal of Pathology and Microbiology, Journal Year: 2023, Volume and Issue: 8(04), P. 90 - 98

Published: April 26, 2023

Host genetics of COVID-19 patients is constantly evolving and may play an important role in the management hospitalized identification new biomarkers. In 2022, numerous studies have been published examining genetic factors that be associated with severe outcomes disease, as well different biomarkers suggested for early diagnosis SARS-CoV-2 infection. this literature review, we provide relevant updated analyses, 2022 correlate what was previous years focused on host or fatal forms COVID-19, Studies using genotyping sequencing techniques polymorphisms promising results genes Renin angiotensin system, Interferon ABO Apolipoprotein E, Dipeptidyl petptidase, Leucine Zipper Transcription Factor-Like Protein 1(LZTFL1) HLA system diverse populations.

Language: Английский

Citations

2