Risk prediction for liver injury in Epstein-Barr virus infection in pediatric respiratory tract infections DOI Creative Commons
Song Mao,

Liangxia Wu,

Wenjing Shi

et al.

˜The œItalian Journal of Pediatrics/Italian journal of pediatrics, Journal Year: 2023, Volume and Issue: 49(1)

Published: Oct. 11, 2023

Epstein-Barr virus (EBV) infection is likely to co-occur in pediatric respiratory tract infections (RTIs). Liver injury the common complication of EBV infection. The detailed risk factors for liver remain elusive. We aimed investigate incidence, characteristics and potential early prediction.We retrospectively recruited RTIs cases with according a predefined criteria from our hospital between January 2015 December 2017. extracted clinical laboratory data electronical medical records. impact age, gender, various parameters on was investigated. Univariate logistic regression analysis performed analyse association clinical/laboratory injury. related indexes were enrolled multivariate analysis. Decision curve used yield value predicting Receiver operating (ROC) applied produce C-index white blood cell (WBC) count also tested non-linear WBC alanine aminotransferase (ALT).A total 216 enrolled. more occur during winter season. Cytomegalovirus independently associated (OR = 6.972, 95% CI 1.648-29.490, p 0.008). 1.169, 1.051-1.301, 0.004). P interaction cytomegalovirus 0.149. decision showed that had larger area under compared platelet (PLT) birthweight (BW). ROC yielded c-index count: 0.75 cut-point 8.3. turning point its ALT 16.8. before after < 0.001 0.123, respectively.Cytomegalovirus co-infection demonstrated 5.972 times an independent biomarker 16.8 More attention should be paid winter. merit monitoring possible among RTIs.

Language: Английский

The probable progression of Epstein-Barr virus (EBV) to chronic active EBV/reactivation weakens the immune response and stimulates Cryptococcus neoformans infection, which invariably proves fatal: a case report and review of the literature DOI Creative Commons
G. C. Mishra,

Gaurav Sarnaik,

Joydeep Samanta

et al.

Frontiers in Virology, Journal Year: 2025, Volume and Issue: 4

Published: Jan. 28, 2025

We have reported here the fatal outcome of Epstein-Barr virus (EBV) infection in a 58-year-old male who had probably developed reactivation/chronic active EBV (CAEBV) which gave rise to various neurological deficits, pancytopenia, and lower CD4 count patient. The decreased immune response helped Cryptococcus neoformans ( C. ) manifest disseminated infection. Although he was exclusively provided with antifungal treatment patient appeared be successfully treated for cryptococcal infection, no coverage detrimental as died very next day. This report highlights need clinical suspicion unexplained cases manifestation, hematological disorder count, multiorgan involvement such pleural effusion, coarse liver echotexture, splenomegaly.

Language: Английский

Citations

0
The viral landscape in metastatic solid cancers DOI Creative Commons
Robin Mjelle, Ícaro Maia Santos de Castro, Kristin Roseth Aass

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(4), P. e42548 - e42548

Published: Feb. 1, 2025

Here, we analyze the viral landscape in blood and tissue from 4,918 metastatic cancer patients across 38 solid types Hartwig Medical Foundation (HMF) cohort, largest pan-cancer study on cancer. Using a coverage-based filtering approach, detected 25 unique genera 32 different types, with total of 747 virus-positive samples. We 336 samples 29 dominated by Torque teno virus Alphatorquevirus. The were Alphapapillomavirus Roseolovirus. was significantly enriched genital, anal, colorectal cancers associated host mutational signatures transcriptional programs related to immunity DNA repair. Host genes integration tended be more highly expressed HPV had higher somatic mutation rates number extrachromosomal elements. also significant proportion cervix anal cancers, suggesting potential blood-based biomarker.

Language: Английский

Citations

0
T cell receptor usage and epitope specificity amongst CD8+ and CD4+ SARS-CoV-2-specific T cells DOI Creative Commons
Ulrik Fahnøe, Shan Feng, Alexander P. Underwood

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 28, 2025

Introduction The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome 2 (SARS-CoV-2), has highlighted the critical importance of understanding protective long-lasting immune responses. This study investigates epitope specificity, T cell receptor (TCR) usage, and phenotypic changes in SARS-CoV-2-specfic CD8 + CD4 cells over time convalescent individuals with COVID-19. Methods Peripheral blood mononuclear (PBMCs) were collected from 28 unvaccinated primary SARS-CoV-2 infection (6 identified as D614G variant, clade 20C) analyzed up to 12 months post-symptom onset. Antigen-specific using flow cytometry single-cell RNA sequencing (scRNAseq) specific dextramer antibody reagents. TCR clonotypes activation markers characterized explore dynamics. Results SARS-CoV-2-specific exhibited waning frequencies long-term, transitioning memory-like a naïve-like state. scRNAseq revealed specificity against both spike non-spike antigens increased CD95 CD127 expression time, indicating that may represent stem memory cells, which are multipotent self-renewing, likely important for long-lived immunity. clonal expansion was observed mainly overlapping beta chain (TRB)-complementary determining region 3 (CDR3) sequences between participants, suggesting shared public epitope-specific repertoires SARS-CoV-2. Further, unique spike-specific high identified, play crucial role long-term protection. Discussion highlights epitope-specificity heterogeneity, some immunodominant responses, suggests potential Shared offers insights into cross-reactive clones, providing valuable information optimizing vaccine strategies emerging variants. findings underscore cellular immunity protection emphasizes

Language: Английский

Citations

0
High Epstein-Barr Virus DNA Load in T Cells Predicts Hemophagocytic Lymphohistiocytosis DOI
Xiaohui Fang, Shanshan Xu,

Kang Cai

et al.

The Journal of Infectious Diseases, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

To evaluate the risk of hemophagocytic lymphohistiocytosis (HLH) linked to Epstein-Barr virus (EBV) infection in different lymphocyte subtypes during infectious mononucleosis (IM). Patients with IM and patients EBV-HLH were included within Children's Critical EBV Infection cohort for a nested case-control study. Lymphocytes isolated into T, B, natural killer cells using magnetic bead sorting, followed by individual polymerase chain reaction testing. Receiver operating characteristic curve analysis identified subtype-specific cutoffs prediction. Kaplan-Meier Cox regression analyses assessed viral load-HLH associations. exhibited significantly higher T-cell loads than (median, 5.1 × 104 vs 6.0 102 copies/106 cells). A load >1.5 was incidences sepsis, renal dysfunction, hepatic coagulation cardiovascular dysfunction (odds ratios, 10.0, 4.7, 6.5, 15.7, 6.5). This elevated strong predictor distinguishing (AUC 0.815) increased developing (hazard ratio 4.7). High DNA T can serve as potential development EBV-HLH.

Language: Английский

Citations

0
Single-cell analysis unveils immune dysregulation and EBV-driven lymphoproliferative disorder in pediatric liver transplant recipients DOI
Yanling Wen, Chen Chen, Tao Zhou

et al.

Human Immunology, Journal Year: 2025, Volume and Issue: unknown, P. 111309 - 111309

Published: April 1, 2025

Language: Английский

Citations

0
Sintilimab treatment for chronic active Epstein–Barr virus infection and Epstein–Barr virus-associated hemophagocytic lymphohistiocytosis in children DOI Creative Commons

Ruyue Chen,

Qiang Lin,

Yun Zhu

et al.

Orphanet Journal of Rare Diseases, Journal Year: 2023, Volume and Issue: 18(1)

Published: Sept. 22, 2023

Chronic active Epstein-Barr virus infection (CAEBV) and virus-associated hemophagocytic lymphohistiocytosis (EBV-HLH) are rare but life-threatening progressive diseases triggered by EBV infection. Glucocorticoid/immunosuppressants treatment is temporarily effective; however, most patients relapse and/or progress. Hematopoietic stem cell transplantation (HSCT) a potentially curative therapy; there risks of transplantation-associated complications. Currently no standard for CAEBV EBV-HLH. Programmed death protein 1 (PD-1) inhibitors have achieved high response in many EBV-related diseases. Sintilimab (a recombinant human IgG4 monoclonal antibody against PD-1) disrupts the interaction between PD-1 its ligand, leading to T reinvigoration.A retrospective analysis was performed on three children with or EBV-HLH Children's Hospital Soochow University 12 December 2020 28 November 2022. The efficacy sintilimab evaluated.Three patients, including two males one female, were analyzed. Among them, diagnosed intermittent fever more than four years, child After mean follow-up 17.1 months (range 10.0-23.3 months), 3 complete clinical patient 2 partial response. All showed > 50% decrease EBV-DNA load both blood plasma. copies sorted T, B, NK cells also markedly decreased after treatment.Our data supported targeted therapy certain EBV-HLH, suggested that could provide cure these diseases, without HSCT. More prospective studies longer needed confirm conclusions.

Language: Английский

Citations

9
Tonsillar cytotoxic CD4 T cells are involved in the control of EBV primary infection in children DOI Creative Commons

María Eugenia Amarillo,

Agustina Moyano, N.M. Ferressini Gerpe

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Jan. 25, 2024

CD4 T cells play a key role in Epstein Barr virus (EBV) infection, by modulating latent antigen expression, and exhibiting cytotoxic regulatory properties. Our aim was to evaluate the presence of Granzyme B (GZMB) Foxp3 at different EBV infection status latency profiles. We examined CD4, GZMB, Foxp3, IL10, TGF-β, CD4-GZMB CD4-Foxp3 expression tonsils pediatric patients with infective CD4+, GZMB+, Foxp3+, CD4-GZMB+ CD4-Foxp3+ cell counts were higher interfollicular region. Higher found primary infected compared healthy carriers. In that expressed III antigens, we demonstrated lower CD4-GZMB+, expression; negative correlation between immunoregulatory cytokine IL-10+ GZMB+ as well positive CD4+. expressing lytic protein BMRF1, TGF-β+ observed. findings indicate are involved restriction patients, which could partially explain lack symptoms, whereas both be modulation latency.

Language: Английский

Citations

3
Predicting autoimmune diseases: A comprehensive review of classic biomarkers and advances in artificial intelligence DOI
Álvaro J. Vivas,

Synda Boumediene,

Gabriel J. Tobón

et al.

Autoimmunity Reviews, Journal Year: 2024, Volume and Issue: 23(9), P. 103611 - 103611

Published: Aug. 28, 2024

Language: Английский

Citations

3
Research Advances in Infectious Mononucleosis Caused by Epstein-Barr Virus DOI Open Access
Yuying Wang, Peng Chen

Open Journal of Pediatrics, Journal Year: 2024, Volume and Issue: 14(01), P. 108 - 121

Published: Jan. 1, 2024

Infectious mononucleosis (IM), primarily caused by the Epstein-Barr virus (EBV), manifests as classic triad of fever, pharyngitis, and cervical lymphadenopathy. Severe cases may involve organ damage, most commonly affecting liver. Diagnosis relies on a combination clinical presentation laboratory parameters, with used indicators including EBV-specific antibodies, EBV-DNA load, ratio atypical lymphocytes. Treatment involves symptomatic supportive care, cautious approach to routine use antiviral medications. In recent years, significant research in traditional Chinese medicine has been conducted China, showing promising advancements. This article provides comprehensive review EBV-induced infectious mononucleosis, offering insights for diagnosis treatment.

Language: Английский

Citations

1
The Level of Anti-Viral Antigen-Specific Antibodies to EBNA-1 in the Serum of MS Patients Does not Depend on the Severity of the Disease DOI Creative Commons
Leyla A. Ovchinnikova,

Samir S. Dzhelad,

Т. О. Simaniv

et al.

Doklady Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: 515(1), P. 48 - 51

Published: March 12, 2024

Abstract Multiple sclerosis (MS) is an autoimmune neurodegenerative disease leading to inevitable disability and primarily affecting the young middle-aged population. Recent studies have shown a direct correlation between risk of MS development Epstein–Barr virus (EBV) infection. Analysis titer EBV-specific antibodies among patients with healthy donors Russian population confirmed that characterized by increased level serum IgG binding EBNA-1 (EBV nuclear antigen 1). The number elevated levels EBNA-1-specific does not differ statistically significantly two groups diametrically opposite courses MS: benign or highly active MS. It can be assumed primary link EBV restricted initiation impact its severity.

Language: Английский

Citations

1