Engineered extracellular vesicles promote the repair of acute kidney injury by modulating regulatory T cells and the immune microenvironment DOI Creative Commons
Lulu Xie, Kaiyue Zhang, Kai Pan

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 10, 2025

Acute kidney injury (AKI) is a common and severe clinical condition. However, the underlying mechanisms of AKI have not been fully elucidated, effective treatment options remain limited. Studies shown that immune cells play critical role in AKI, with regulatory T (Tregs) being one most important immunosuppressive lymphocytes. Tregs proliferation can attenuate whereas depletion exacerbates injury. Given endothelial (ECs) are initial interact when they invade tissue parenchyma, ECs closely associated reactions. In this study, P-selectin binding peptide-extracellular vesicles (PBP-EVs) target repair engineered. Transcriptome sequencing reveals PBP-EVs reduce expression inflammatory genes mice. Using high-resolution intravital two-photon microscopy (TPM), an increased recruitment kidneys Foxp3-EGFP transgenic mice following observed, as well significant Lgr5+ renal stem cell Lgr5-CreERT2; R26mTmG Additionally, result reduced infiltration cells, pathological damage fibrosis Upon Foxp3-DTR mice, we observe diminished therapeutic effect on AKI. The experimental results indicate promote regeneration by mitigating subsequently modulating microenvironment. These findings provide novel insights strategies for

Language: Английский

Engineered extracellular vesicles promote the repair of acute kidney injury by modulating regulatory T cells and the immune microenvironment DOI Creative Commons
Lulu Xie, Kaiyue Zhang, Kai Pan

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 10, 2025

Acute kidney injury (AKI) is a common and severe clinical condition. However, the underlying mechanisms of AKI have not been fully elucidated, effective treatment options remain limited. Studies shown that immune cells play critical role in AKI, with regulatory T (Tregs) being one most important immunosuppressive lymphocytes. Tregs proliferation can attenuate whereas depletion exacerbates injury. Given endothelial (ECs) are initial interact when they invade tissue parenchyma, ECs closely associated reactions. In this study, P-selectin binding peptide-extracellular vesicles (PBP-EVs) target repair engineered. Transcriptome sequencing reveals PBP-EVs reduce expression inflammatory genes mice. Using high-resolution intravital two-photon microscopy (TPM), an increased recruitment kidneys Foxp3-EGFP transgenic mice following observed, as well significant Lgr5+ renal stem cell Lgr5-CreERT2; R26mTmG Additionally, result reduced infiltration cells, pathological damage fibrosis Upon Foxp3-DTR mice, we observe diminished therapeutic effect on AKI. The experimental results indicate promote regeneration by mitigating subsequently modulating microenvironment. These findings provide novel insights strategies for

Language: Английский

Citations

1