Extended antibiotic treatment in salmon farms select multiresistant gut bacteria with a high prevalence of antibiotic resistance genes

PLoS ONE, Journal Year: 2018, Volume and Issue: 13(9), P. e0203641 - e0203641

Published: Sept. 11, 2018

The high use of antibiotics for the treatment bacterial diseases is one main problems in mass production animal protein. Salmon farming Chile a clear example above statement, where more than 5,500 tonnes have been used over last 10 years. This has caused great impact both at level and on environment; however, there are still few works relation to it. In order demonstrate fish gut microbiota, we selected four salmon farms presenting similar amount Atlantic species (Salmo salar), ranging from 4,500 6,000 tonnes. All these treatments with doses antibiotics. Thus, 15 healthy were euthanised isolate bacteria resistant oxytetracycline florfenicol microbiota. total, 47 isolates 44 isolated, among which Minimum Inhibitory Concentrations (MIC) exceeding 2048 μg/mL 1024 found. addition, another six different tested multiresistance phenomenon. this regard, 91 showed elevated resistance values eight antibiotics, including oxytetracycline, These called "super-resistant" bacteria. phenotypic was verified genotypic since most antibiotic genes (ARGs) oxytetracycline. Specifically, 77% least gene 89% present study, it demonstrated that has, as consequence, selection multiresistant microbiota farmed Salmo salar seawater stage. Also, can be correlated presence genes.

Language: Английский

---

Enrichment of antibiotic resistance genes after sheep manure aerobic heap composting

Bioresource Technology, Journal Year: 2021, Volume and Issue: 323, P. 124620 - 124620

Published: Jan. 1, 2021

Language: Английский

---

Giant Transposons in Eukaryotes: Is Bigger Better?

Genome Biology and Evolution, Journal Year: 2019, Volume and Issue: 11(3), P. 906 - 918

Published: Feb. 22, 2019

Transposable elements (TEs) are ubiquitous in both prokaryotes and eukaryotes, the dynamic character of their interaction with host genomes brings about numerous evolutionary innovations shapes genome structure function a multitude ways. In traditional classification systems, TEs often being depicted simplistic ways, based primarily on key enzymes required for transposition, such as transposases/recombinases reverse transcriptases. Recent progress whole-genome sequencing long-read assembly, combined expansion familiar range model organisms, resulted identification unprecedentedly long transposable units spanning dozens or even hundreds kilobases, initially prokaryotic more recently eukaryotic systems. Here, we focus oversized TEs, including retrotransposons DNA transposons, outline complex combinatorial nature closely intertwined relationship viruses, discuss potential participating transfer stretches eukaryotes.

Language: Английский

---

Review of Antibiotic Resistance, Ecology, Dissemination, and Mitigation in U.S. Broiler Poultry Systems

Frontiers in Microbiology, Journal Year: 2019, Volume and Issue: 10

Published: Nov. 15, 2019

Since the onset of land application poultry manure and residues, transportation microorganisms, antibiotics, disinfectants to new locations has occurred. While some studies provide evidence that antimicrobial resistance (AMR), an evolutionary phenomenon, could be influenced by animal production systems, other research suggests AMR originates in environment from non-anthropogenic sources. In addition, impacts effective prevention treatment illnesses is increasingly a threat global public health. Therefore, there need understand dissemination genes environment, particularly those directly relevant human health using One Health Approach. This review focuses on potential movement soil via litter. Additionally, we highlight microbial ecology explore hypotheses explaining gene pathways U.S. broiler operations environment. Current approaches for decreasing antibiotic use are also described this review.

Language: Английский

---

Occurrence of emerging sulfonamide resistance (sul1 and sul2) associated with mobile integrons-integrase (intI1 and intI2) in riverine systems

The Science of The Total Environment, Journal Year: 2020, Volume and Issue: 751, P. 142217 - 142217

Published: Sept. 9, 2020

Language: Английский

---

Antibiotic resistance in plant growth promoting bacteria: A comprehensive review and future perspectives to mitigate potential gene invasion risks

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Sept. 20, 2022

Plant growth-promoting bacteria (PGPB) are endowed with several attributes that can be beneficial for host plants. They opened myriad doors toward green technology approach to reduce the use of chemical inputs, improve soil fertility, and promote plants’ health. However, many these PGPB harbor antibiotic resistance genes (ARGs). Less attention has been given multi-resistant bacterial bioinoculants which may transfer their ARGs native microbial communities other environmental reservoirs including animals, waters, humans. Therefore, large-scale inoculation crops by ARGs-harboring could worsen evolution dissemination aggravate negative impacts on such ecosystem ultimately public Their introduction into serve as invasion inter food chain. In this review, we underscore plant-associated bacteria, criticize lack consideration phenomenon in screening application processes, provide some recommendations well a regulation framework relating development bacteria-based biofertilizers aid maximizing value applications crop improvement while reducing risks invasion.

Language: Английский

---

Mobile genetic elements in Acinetobacter antibiotic‐resistance acquisition and dissemination

Annals of the New York Academy of Sciences, Journal Year: 2022, Volume and Issue: 1518(1), P. 166 - 182

Published: Oct. 31, 2022

Abstract Pathogenic Acinetobacter species, most notably baumannii , are a significant cause of healthcare‐associated infections worldwide. particular concern to global health due the high rates multidrug resistance and extensive drug resistance. Widespread genome sequencing analysis has determined that bacterial antibiotic is often acquired disseminated through movement mobile genetic elements, including insertion sequences (IS), transposons, integrons, conjugative plasmids. In specifically, carbapenems cephalosporins highly correlated with IS, as many IS Aba elements encode strong outwardly facing promoters required for sufficient expression β‐lactamases confer clinical Here, we review role in species framework mechanism acquisition focus on experimentally validated mechanisms.

Language: Английский

---

Xenogenetic evolutionary of integrons promotes the environmental pollution of antibiotic resistance genes — Challenges, progress and prospects

Water Research, Journal Year: 2023, Volume and Issue: 231, P. 119629 - 119629

Published: Jan. 17, 2023

Language: Английский

---

Profile and resistance levels of 136 integron resistance genes

npj Antimicrobials and Resistance, Journal Year: 2023, Volume and Issue: 1(1)

Published: Oct. 11, 2023

Integrons have played a major role in the rise and spread of multidrug resistance Gram-negative pathogens are nowadays commonplace among clinical isolates. These platforms capture, stockpile, modulate expression more than 170 antimicrobial cassettes (ARCs) against most clinically-relevant antibiotics. Despite their importance, our knowledge on profile levels is patchy, because data scattered literature, often reported different genetic backgrounds sometimes extrapolated from sequence similarity alone. Here we generated collection 136 ARCs 8 antibiotic families disinfectants. Cassettes cloned vector designed to mimic environment class 1 integron, transformed Escherichia coli. We measured minimal inhibitory concentration (MIC) relevant molecules each family. With 500 MIC values, provide an exhaustive comparable quantitation conferred by ARCs. Our confirm known trends profiles while revealing important differences closely related genes. also detected genes that do not confer expected resistance, point challenging whole family qac work provides detailed characterization integron at-a-glance.

Language: Английский

---

Hijackers, hitchhikers, or co-drivers? The mysteries of mobilizable genetic elements

PLoS Biology, Journal Year: 2024, Volume and Issue: 22(8), P. e3002796 - e3002796

Published: Aug. 29, 2024

Mobile genetic elements shape microbial gene repertoires and populations. Recent results reveal that many, possibly most, mobile require helpers to transfer between genomes, which we refer as Hitcher Genetic Elements (hitchers or HGEs). They may be a large fraction of pathogenicity resistance genomic islands, whose mechanisms have remained enigmatic for decades. Together with their helper bacterial hosts, hitchers form tripartite networks interactions evolve rapidly within parasitism–mutualism continuum. In this emerging view genomes communities many questions arise. Which are being moved, by whom, how? How often costly hyper-parasites beneficial mutualists? What is the evolutionary origin hitchers? Are there key advantages associated hitchers’ lifestyle justify unexpected abundance? And why systematically smaller than helpers? essay, start answering these point ways ahead understanding principles, origin, mechanisms, impact in ecology evolution.

Language: Английский

---