Clinical burden of Acinetobacter baumannii, including carbapenem-resistant A. baumannii, in hospitalized adult patients in the USA between 2018 and 2022 DOI Creative Commons
Thomas P. Lodise, Sean Nguyen, Caroline Margiotta

et al.

BMC Infectious Diseases, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 17, 2025

Abstract Background Epidemiological data for United States (US) hospitals regarding the burden of Acinetobacter baumannii and carbapenem-resistant A. (CRAB) are scarce; thus, this study aimed to describe incidence CRAB across US between January 1, 2018 December 31, 2022. Methods This was a retrospective cohort hospitalized patients with microbiology from PINC AI™ Database. Incidence rates (January – 2022) were determined in each census region. at hospitalization encounter individual levels. Presence based on non-susceptibility either doripenem, imipenem, or meropenem. Patient demographics, comorbidities, outcomes, including in-hospital mortality, compared carbapenem-susceptible (CSAB) level. Results During period, 7,270 encounters ≥ 1 clinical cultures identified. The overall rate 1.19 cases per 100 1.33 unique patients. For CRAB, total 2,708 identified, 0.44 encounters. West South Central, East North Central regions had highest (0.78, 0.67, 0.63 encounters, respectively). Compared CSAB, significantly more positive (20.9% vs. 10.0%, respectively, P < 0.0001) higher prevalence other Gram-negative pathogens any culture site within ± 3 days index (47.2% 42.9%, = 0.0004). Patients incidences mortality CSAB (20.5% 11.3%, 0.0001). Conclusions identified 1% adult hospitalizations multicenter study. Over third observed most central regions. Clinicians should consider as potential pathogen an increasing .

Language: Английский

Microplastics Enhance the Prevalence of Antibiotic Resistance Genes in Anaerobic Sludge Digestion by Enriching Antibiotic-Resistant Bacteria in Surface Biofilm and Facilitating the Vertical and Horizontal Gene Transfer DOI
Tian‐Yi Luo, Xiaohu Dai, Wei Wei

et al.

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(39), P. 14611 - 14621

Published: Sept. 21, 2023

Antibiotic resistance genes (ARGs) and microplastics (MPs) are recognized as emerging contaminants threats to global human health. Despite both of them being significantly detected in their "hotspots", i.e., waste activated sludge (WAS), rare studies on how MPs affect ARGs antibiotic-resistant bacteria (ARB) anaerobic digestion available. Herein, the fate ARB after exposure three dosages (10, 30, 80 particles/g-TS), polymer types (LDPE, PET, PS), branching extents LLDPE, HDPE) was investigated. Metagenomic results indicated that all variants resulted an increase relative abundance digester compared control. The demonstrated a dosage-dependent relationship within range from 10 particles/g-TS, resulting 4.5 27.9% Branching structure type influence ARG level well. Mechanism revealed LDPE selectively enriched potential surface biofilm, possibly creating favorable environment for proliferation exchange. Furthermore, vertical transfer facilitated by through increasing bacterial cell accompanied enhancement relevant functional genes. elevated mobile genetic elements (MGEs) ARGs-carrying plasmids also MGE-mediated horizontal promoted at particles/g-TS. This effect compounded increased oxidative stress, membrane permeability, cohesion, collectively facilitating transfer. Consequently, could be concurrently digester.

Language: Английский

Citations

51
Pan-Genome Plasticity and Virulence Factors: A Natural Treasure Trove for Acinetobacter baumannii DOI Creative Commons
Theodoros Karampatakis, Katerina Tsergouli, Payam Behzadi

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(3), P. 257 - 257

Published: March 14, 2024

Acinetobacter baumannii is a Gram-negative pathogen responsible for variety of community- and hospital-acquired infections. It recognized as life-threatening among hospitalized individuals and, in particular, immunocompromised patients many countries. A. baumannii, member the ESKAPE group, encompasses high genomic plasticity simultaneously predisposed to receive exchange mobile genetic elements (MGEs) through horizontal transfer (HGT). Indeed, treasure trove that contains number virulence factors. In accordance with these unique pathogenic characteristics authors aim discuss natural pan-genome factors pertaining this bacterial monster try highlight reasons why bacterium great concern global public health system.

Language: Английский

Citations

26
Mobile genetic elements in Acinetobacter antibiotic‐resistance acquisition and dissemination DOI Creative Commons
Hannah R. Noel, Jessica R. Petrey, Lauren D. Palmer

et al.

Annals of the New York Academy of Sciences, Journal Year: 2022, Volume and Issue: 1518(1), P. 166 - 182

Published: Oct. 31, 2022

Abstract Pathogenic Acinetobacter species, most notably baumannii , are a significant cause of healthcare‐associated infections worldwide. particular concern to global health due the high rates multidrug resistance and extensive drug resistance. Widespread genome sequencing analysis has determined that bacterial antibiotic is often acquired disseminated through movement mobile genetic elements, including insertion sequences (IS), transposons, integrons, conjugative plasmids. In specifically, carbapenems cephalosporins highly correlated with IS, as many IS Aba elements encode strong outwardly facing promoters required for sufficient expression β‐lactamases confer clinical Here, we review role in species framework mechanism acquisition focus on experimentally validated mechanisms.

Language: Английский

Citations

46
Impact of COVID-19 pandemic on profiles of antibiotic-resistant genes and bacteria in hospital wastewater DOI Open Access
Liang Zhao,

Ziquan Lv,

Liangqiang Lin

et al.

Environmental Pollution, Journal Year: 2023, Volume and Issue: 334, P. 122133 - 122133

Published: July 1, 2023

Language: Английский

Citations

26
Targeting Acinetobacter baumannii resistance-nodulation-division efflux pump transcriptional regulators to combat antimicrobial resistance DOI Creative Commons
Ruwani L. Wimalasekara,

Dawn White,

Ayush Kumar

et al.

npj Antimicrobials and Resistance, Journal Year: 2025, Volume and Issue: 3(1)

Published: Jan. 25, 2025

Regulatory elements controlling gene expression fine-tune bacterial responses to environmental cues, including antimicrobials, optimize survival. Acinetobacter baumannii, a pathogen notorious for antimicrobial resistance, relies on efficient efflux systems. Though the role of systems in antibiotic expulsion are well recognized, regulatory mechanisms their remain understudied. This review explores current understanding these regulators, aiming inspire strategies combat resistance and improve therapeutic outcomes.

Language: Английский

Citations

1
Epidemiological characteristics and antimicrobial resistance of extensively drug-resistant Acinetobacter baumannii in ICU wards DOI Creative Commons
Jingchao Shi,

Xiaoting Mao,

Fengtian Sun

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

ABSTRACT Acinetobacter baumannii is a significant nosocomial pathogen, particularly problematic due to its extensive drug resistance. This study investigates 56 extensively drug-resistant A. (XDRAB) strains collected from various ICU wards at Jinhua Central Hospital, Zhejiang Province, China. Strains were isolated diverse clinical samples, including sputum, blood, cerebrospinal fluid, and wound secretions. Identification was confirmed via matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS), antibiotic susceptibility testing conducted using the VITEK 2 Compact system, E-test, Kirby-Bauer methods. All susceptible polymyxin, with four showing intermediate tigecycline, while resistance rates other antibiotics 100%. Molecular typing through pulsed-field gel electrophoresis (PFGE) classified into 10 types, dominant type (G) primarily found in ICU3, indicating potential clonal outbreak. Whole-genome sequencing (WGS) multi-locus sequence (MLST) identified ST208 as predominant type. Resistance gene screening revealed presence blaOXA-23, blaTEM-1D, aminoglycoside genes most strains. Phylogenetic analysis transmission across hospital, high degree genomic similarity among isolates. These findings highlight importance continuous monitoring effective infection control measures prevent spread XDRAB healthcare settings. IMPORTANCE Extensively critical public health threat, hospital environments where it causes variety infections. The global make treatment options limited, increasing risk patient morbidity mortality. provides important insights molecular epidemiology setting, revealing By utilizing both whole-genome (WGS), we genetic links between key genes. underscore urgent need for robust protocols, routine surveillance, judicious use mitigate ensure better outcomes.

Language: Английский

Citations

1
Importance of mobile genetic elements for dissemination of antimicrobial resistance in metagenomic sewage samples across the world DOI Creative Commons
Markus Hans Kristofer Johansson, Frank M. Aarestrup, Thomas Nordahl Petersen

et al.

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(10), P. e0293169 - e0293169

Published: Oct. 19, 2023

We are facing an ever-growing threat from increasing antimicrobial resistance (AMR) in bacteria. To mitigate this, we need a better understanding of the global spread genes (ARGs). ARGs often among bacteria by horizontal gene transfer facilitated mobile genetic elements (MGE). Here use dataset consisting 677 metagenomic sequenced sewage samples 97 countries or regions to study how MGEs geographically distributed and they disseminate worldwide. The ARGs, MGEs, bacterial abundance were calculated reference-based read mapping. found systematic differences where some prevalent on all continents while others had higher separate geographic areas. Different tended be localized temperate tropical climate zones, different according continents. This suggests that is important factor influencing local flora MGEs. also more confined than ARGs. identified several integrated whose correlated with genera, indicating ability mobilize these genes. Some seemed able species. host ranges differ between elements, most associated same family. believe our method could used investigate population dynamics complex populations.

Language: Английский

Citations

17
Recovery and genetic characterization of clinically-relevant ST2 carbapenem-resistant Acinetobacter baumannii isolates from untreated hospital sewage in Zhejiang Province, China DOI Creative Commons
Danxia Gu, Yuchen Wu, Kaichao Chen

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 916, P. 170058 - 170058

Published: Jan. 12, 2024

The global transmission of carbapenem-resistant Acinetobacter baumannii (CRAB) poses a significant and grave threat to human health. To investigate the potential relationship between hospital sewage CRAB within healthcare facilities, isolates spp. obtained from untreated samples were subjected antimicrobial susceptibility tests, genome sequencing, bioinformatic phylogenetic tree analysis, that data matched with those clinical isolates. Among 70 tested, A. was most prevalent detectable in 5 hospitals, followed by nosocomialis gerneri. Worryingly, 57.14 % (40/70) MDR, 25.71 (18/70) being resistant carbapenem. When utilizing Pasteur scheme, ST2 predominant type among these isolates, Tn2006 (ΔISAba1-bla

Language: Английский

Citations

6
Roles and fates of antibiotics in anaerobic digestion of waste activated sludge: Insights to pro- and re-duction of antibiotic resistance genes DOI

Q. Meng,

Zhang-Wei He,

Wenjing Yang

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 500, P. 156633 - 156633

Published: Oct. 11, 2024

Language: Английский

Citations

6
Heteroresistance to cefiderocol in carbapenem-resistant Acinetobacter baumannii in the CREDIBLE-CR study was not linked to clinical outcomes: a post hoc analysis DOI Creative Commons
Christopher Longshaw, Anne Santerre Henriksen,

Dana C. Dressel

et al.

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(6)

Published: Nov. 15, 2023

The population analysis profiling (PAP) test is considered the "gold standard" method to detect heteroresistance. It exposes bacteria increasing concentrations of antibiotics at high cell densities any minority resistant subpopulations that might be missed by low inoculums used for reference susceptibility tests. However, its clinical relevance has not been well established. In CREDIBLE-CR study, a numerically increased all-cause mortality was observed in cefiderocol arm relative best available therapy patients with Acinetobacter spp. infections. Heteroresistance independently proposed another research group as potential explanation difference. An baseline carbapenem-resistant calcoaceticus-baumannii complex isolates from treated study showed highest cure rate and lowest PAP-heteroresistant compared PAP-susceptible or PAP-resistant isolates. These findings contradict abovementioned hypothesis heteroresistance contributed mortality.

Language: Английский

Citations

14