A mutualistic interaction between Streptomyces bacteria, strawberry plants and pollinating bees

Nature Communications, Journal Year: 2019, Volume and Issue: 10(1)

Published: Oct. 22, 2019

Microbes can establish mutualistic interactions with plants and insects. Here we track the movement of an endophytic strain Streptomyces bacteria throughout a managed strawberry ecosystem. We show that isolate found in rhizosphere on flowers protects both plant pollinating honeybees from pathogens (phytopathogenic fungus Botrytis cinerea pathogenic bacteria, respectively). The pollinators transfer among plants, move into vascular bundle rhizosphere. Our results present tripartite mutualism between Streptomyces, pollinator partners.

Language: Английский

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Rhizosheath microbial community assembly of sympatric desert speargrasses is independent of the plant host

Microbiome, Journal Year: 2018, Volume and Issue: 6(1)

Published: Dec. 1, 2018

The rhizosheath-root system is an adaptive trait of sandy-desert speargrasses in response to unfavourable moisture and nutritional conditions. Under the deserts' polyextreme conditions, plants interact with edaphic microorganisms that positively affect their fitness resistance. However, trophic simplicity environmental harshness desert ecosystems have previously been shown strongly influence soil microbial community assembly. We hypothesize sand-driven ecological filtering constrains recruitment processes speargrass niche, prevailing over plant-induced selection. Bacterial fungal communities from compartments (endosphere root tissues, rhizosheath rhizosphere) three Namib Desert species (Stipagrostis sabulicola, S. seelyae Cladoraphis spinosa) along bulk sand studied test our hypothesis. To minimize variability determined by climatic factors, living a single dune were studied. assessed role plant vs sandy substrate on selection, phylogenetic diversity co-occurrence networks communities. Microorganisms associated recruited surrounding population significantly enriched (105 104 bacterial 16S rRNA ITS copies per gram up 108 107 gram, respectively). Furthermore, each compartment hosted specific demonstrating strong niche-partitioning. systems dominated desert-adapted Actinobacteria Alphaproteobacteria (e.g. Lechevalieria, Streptomyces Microvirga) as well saprophytic Ascomycota fungi Curvularia, Aspergillus Thielavia). Our results clearly showed random turnover communities, independent species, where stochastic factors drive neutral Co-occurrence network analyses also indicated members established higher number interactions than those barren sand, suggesting former are more stable functional latter. study demonstrates stochastically assembled host-independent. This finding supports concept selection prevails imposed genotype different species.

Language: Английский

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Seeds of native alpine plants host unique microbial communities embedded in cross-kingdom networks

Microbiome, Journal Year: 2019, Volume and Issue: 7(1)

Published: July 24, 2019

The plant microbiota is crucial for health and growth. Recently, vertical transmission of a beneficial core was identified crop seeds, but native plants, complementary mechanisms are almost completely unknown. We studied the seeds eight species growing together centuries under same environmental conditions in Alpine meadows (Austria) by qPCR, FISH-CLSM, amplicon sequencing targeting bacteria, archaea, fungi. Bacteria fungi were determined with approx. 1010 gene copy numbers g−1 seed as abundant inhabitants. Archaea, which newly discovered endophytes, less represent only 1.1% signatures. microbiome highly diversified, all showed species-specific, unique microbial signature, sharing an exceptionally small microbiome. genotype (species) clearly main driver, while different life cycles (annual/perennial) had impact on composition, fruit morphology (capsule/achene) no significant impact. A network analysis revealed co-occurrence patterns bacteria contrasting independent fungal that dominated mutual exclusions. These novel insights into contribute to deeper understanding diversity phytopathological processes health, beyond ecosystem plasticity diversification within plant-specific microbiota.

Language: Английский

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The Seagrass Holobiont and Its Microbiome

Microorganisms, Journal Year: 2017, Volume and Issue: 5(4), P. 81 - 81

Published: Dec. 15, 2017

Seagrass meadows are ecologically and economically important components of many coastal areas worldwide. Ecosystem services provided by seagrasses include reducing the number microbial pathogens in water, providing food, shelter nurseries for species, decreasing impact waves on shorelines. A global assessment reported that 29% known areal extent has disappeared since seagrass were initially recorded 1879. Several factors such as direct indirect human activity contribute to demise seagrasses. One main reasons die-offs all over world is increased sulfide concentrations sediment result from sulfate-reducing prokaryotes, which perform last step anaerobic food chain marine sediments reduce sulfate H2S. Recent die-offs, e.g., Florida Biscayne Bays, caused an increase pore-water sediment, combined unfavorable environmental conditions activities various groups heterotrophic bacteria sulfate-rich water-column stimulated through nutrient concentrations. Under normal circumstances, able withstand low levels sulfide, probably partly due symbionts, detoxify oxidizing it sulfur or sulfate. Novel studies beginning give greater insights into interactions microbes seagrasses, not only cycle. Here, we review literature basic ecology biology focus describing their microbiome.

Language: Английский

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Seed‐borne, endospheric and rhizospheric core microbiota as predictors of plant functional traits across rice cultivars are dominated by deterministic processes

New Phytologist, Journal Year: 2021, Volume and Issue: 230(5), P. 2047 - 2060

Published: Feb. 24, 2021

Summary A host‐plant and its associated microbiota depend on one another. However, the assembly process functioning of host‐associated are poorly understood. Herein, rice was used as model plant to investigate assemblage bacterial microbiota, including those in seed, root endosphere rhizosphere. We also assessed degree which rhizosphere communities were influenced by vertical transmission through seed identified core microbes that potentially with phenotypic properties. Plant microhabitat, rather than subspecies type, major driver shaping plant‐associated microbiota. Deterministic processes primarily responsible for community all microhabitats. The influence from root‐associated appeared be quite weak (endosphere) or even absent (rhizosphere). microbial composed 15 generalist species persisted across different microhabitats represented key connectors networks. Host‐plant functional traits linked relative abundance these could predicted them using machine learning algorithms. Overall, is assembled interactions a deterministic‐based manner. This study enhances our understanding driving mechanisms associations various provides new perspectives improve performance.

Language: Английский

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Engineering banana endosphere microbiome to improve Fusarium wilt resistance in banana

Microbiome, Journal Year: 2019, Volume and Issue: 7(1)

Published: May 15, 2019

Plant microbiome highlights the importance of endosphere for growth and health host plant. Microbial community analysis represents an elegant way to identify keystone microbial species that have a more central position in community. The aim this study was access interactions between bacterial plants during banana Fusarium wilt process, by comparing endophytic fungal roots shoot tips wilting processes. were isolated further engineered improve resistance. Banana structure varied plant Bacterial diversity increased with development plantlets. groups belonging Enterobacteriaceae family different relative abundances detected all samples. Klebsiella spp. might be bacteria abundance associated disease did not increase process. strains Enterobacter sp. E5, Kosakonia S1, Kb on selective medium expressing 1-aminocyclopropane-1-carboxylate (ACC) deaminase cell walls (designated as E5P, S1P, KbP, respectively). Pot experiments suggested inoculated S1P resistance compared controls without inoculation, whereas inoculation (Kb KbP) Compared wild E5 E5P significantly promoted growth, respectively. results illustrated may dominant numbers functional role should involved ACC activity essential promotion plants. Engineering promising method

Language: Английский

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Identification and Characterization of the Core Rice Seed Microbiome

Phytobiomes Journal, Journal Year: 2019, Volume and Issue: 3(2), P. 148 - 157

Published: Jan. 1, 2019

The use of microbes in agriculture for enhancing crop production is an emerging alternative to chemical fertilizers and pesticides; however, their effectiveness often limited by factors such as host genotype variability geographic location. To address this issue, the microbiomes six different rice (Oryza sativa) seeds, sourced from two locations Arkansas, U.S.A. genotypes harvest years, were characterized. bacterial fungal communities identified each four seed compartments (grain, outer grain, husk, husk) using high throughput Illumina MiSeq sequencing. More unique amplicon sequence variants husk least grain compartment both microbiomes, however only resulted a decrease diversity communities. Principal component analysis indicated that tissue harbored relatively distinct three innermost compartments. A core microbiome shared among types was identified. Key genera across all Sphingomonas, Methylobacterium, taxa family Enterobacteriaceae, members which have been reported support growth. Compared with core, more identified, possibly resulting abundant reads after filtering, key Alternaria, Hannaella, order Pleosporales. These represent valuable candidates manipulating microbiome, decreasing chemicals while increasing plant performance.

Language: Английский

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Microbiome approaches provide the key to biologically control postharvest pathogens and storability of fruits and vegetables

FEMS Microbiology Ecology, Journal Year: 2020, Volume and Issue: 96(7)

Published: June 14, 2020

ABSTRACT Microbes play an important role in plants and interact closely with their host starting from sprouting seeds, continuing during growth after harvest. The discovery of importance for plant postharvest health initiated a biotechnological development various antagonistic bacteria fungi disease control. Nevertheless, application often showed inconsistent effects. Recently, high-throughput sequencing-based techniques including advanced microscopy reveal fruits vegetables as holobionts. At harvest, all harbor highly abundant specific microbiota beneficial, pathogenic spoilage microorganisms. Especially, high microbial diversity resilient networks were shown to be linked fruit vegetable health, while diseased products severe dysbiosis. Field handling was affect the indigenous microbiome therefore has substantial impact on storability vegetables. Microbiome tracking can implemented new tool evaluate assess processes contribute health. Here, we summarize current research advancements emerging field microbiomes elaborate its importance. generated knowledge provides profound insights into dynamics sets basis targeted, microbiome-driven sustainable control strategies.

Language: Английский

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Relevance of Plant Growth Promoting Microorganisms and Their Derived Compounds, in the Face of Climate Change

Agronomy, Journal Year: 2020, Volume and Issue: 10(8), P. 1179 - 1179

Published: Aug. 12, 2020

Climate change has already affected food security in many parts of the world, and this situation will worsen if nothing is done to combat it. Unfortunately, agriculture a meaningful driver climate change, through greenhouse gas emissions from nitrogen-based fertilizer, methane animals animal manure, as well deforestation obtain more land for agriculture. Therefore, global agricultural sector should minimize order slow change. The objective review point out various ways plant growth promoting microorganisms (PGPM) can be used enhance crop production amidst challenges, effects on conventional such as: weeds, pests, pathogens, salinity, drought, etc. Current knowledge regarding microbial inoculant technology discussed. Pros cons single inoculants, consortia compounds are A range microbes microbe derived that have been reported biotic abiotic stresses, microbe-based products market agroinputs, focus. This provide reader with clearer understanding current trends inoculants how they challenges.

Language: Английский

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Next generation microbiome applications for crop production — limitations and the need of knowledge-based solutions

Current Opinion in Microbiology, Journal Year: 2019, Volume and Issue: 49, P. 59 - 65

Published: June 1, 2019

Language: Английский

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