Synergic interactions between Trichoderma and the soil microbiomes improve plant iron availability and growth DOI Creative Commons
Yafang Shao, Shaohua Gu, H. Peng

et al.

npj Biofilms and Microbiomes, Journal Year: 2025, Volume and Issue: 11(1)

Published: April 8, 2025

Iron bioavailability is often limited especially in calcareous soils. Trichoderma harzianum strongly improves plant iron uptake and growth However, little known about the mechanisms by which T. mobilizes Here, model strain NJAU4742 a synthetic microbial community (SynCom) was used to show that efficacy of enhancing nutrition soils depends on soil microbiome. Enhanced iron-mobilization functions SynCom were observed presence NJAU4742. Concurrently, improved capacity enriching strains are able do so. Finally, Chryseobacterium populi identified as key driver mobilization, while their synergistic colonization further enhances this process. This study unveils pivotal mechanism NJAU4742-mediated re-structuring microbiome ameliorates nutrition.

Language: Английский

Microbial interactions for nutrient acquisition in soil: Miners, scavengers, and carriers DOI
Tingting Cao, Yunchao Luo, Man Shi

et al.

Soil Biology and Biochemistry, Journal Year: 2023, Volume and Issue: 188, P. 109215 - 109215

Published: Oct. 24, 2023

Language: Английский

Citations

80
Adaptive laboratory evolution reveals regulators involved in repressing biofilm development as key players in Bacillus subtilis root colonization DOI Creative Commons
Maude Pomerleau, Vincent Charron‐Lamoureux,

Lucille Léonard

et al.

mSystems, Journal Year: 2024, Volume and Issue: 9(2)

Published: Jan. 11, 2024

Root-associated microorganisms play an important role in plant health, such as growth-promoting rhizobacteria (PGPR) from the

Language: Английский

Citations

16
Identifying microbiota community patterns important for plant protection using synthetic communities and machine learning DOI Creative Commons

Barbara Emmenegger,

Julien Massoni, Christine M. Pestalozzi

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Dec. 2, 2023

Plant-associated microbiomes contribute to important ecosystem functions such as host resistance biotic and abiotic stresses. The factors that determine community outcomes are inherently difficult identify under complex environmental conditions. In this study, we present an experimental analytical approach explore microbiota properties relevant for a microbiota-conferred phenotype, here plant protection, in reductionist system. We screened 136 randomly assembled synthetic communities (SynComs) of five bacterial strains each, followed by classification regression analyses well empirical validation test potential explanatory structure composition, including evenness, total commensal colonization, phylogenetic diversity, strain identity. find identity be the most predictor pathogen reduction, with machine learning algorithms improving performances compared random classifications (94-100% versus 32% recall) non-modelled predictions (0.79-1.06 1.5 RMSE). Further confirms three main drivers reduction two additional confer protection combination. Beyond specific application presented our provide framework can adapted help features function other biological systems.

Language: Английский

Citations

35
Deciphering Microbial Community and Nitrogen Fixation in the Legume Rhizosphere DOI

Yaohui Yang,

Nuohan Xu,

Zhenyan Zhang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(11), P. 5659 - 5670

Published: March 5, 2024

Nitrogen is the most limiting factor in crop production. Legumes establish a symbiotic relationship with rhizobia and enhance nitrogen fixation. We analyzed 1,624 rhizosphere 16S rRNA gene samples 113 metagenomic from three typical legumes non-legumes. The microbial community of had low diversity was enriched nitrogen-cycling bacteria (Sphingomonadaceae, Xanthobacteraceae, Rhizobiaceae, Bacillaceae). Furthermore, microbiota exhibited high abundance nitrogen-fixing genes, reflecting stronger potential, Streptomycetaceae Nocardioidaceae were predominant bacteria. also identified helper confirmed through metadata analysis pot experiment that synthesis riboflavin by key promoting Our study emphasizes construction synthetic communities crucial for development efficient fertilizers.

Language: Английский

Citations

13
Deciphering Differences in Microbial Community Diversity between Clubroot-Diseased and Healthy Soils DOI Creative Commons

Huajun Kang,

Ali Chai, Zihan Lin

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(2), P. 251 - 251

Published: Jan. 25, 2024

Clubroot (Plasmodiophora brassicae) is an important soilborne disease that causes severe damage to cruciferous crops in China. This study aims compare the differences chemical properties and microbiomes between healthy clubroot-diseased soils. To reveal difference, we measured soil microbial communities by sequencing 18S 16S rRNA amplicons. The available potassium diseased soils was higher than fungal diversity significantly Ascomycota Proteobacteria were most dominant phylum bacteria all samples, respectively. Plant-beneficial microorganisms, such as Chaetomium Sphingomonas, more abundant Co-occurrence network analysis found networks complex stable link number, density, clustering coefficient of those networks. Our results indicate community structure different from great significance exploring biological control strategies clubroot disease.

Language: Английский

Citations

9
Seed microbiomes promote Astragalus mongholicus seed germination through pathogen suppression and cellulose degradation DOI Creative Commons
Da Li, Weimin Chen, Wen Luo

et al.

Microbiome, Journal Year: 2025, Volume and Issue: 13(1)

Published: Jan. 24, 2025

Seed-associated microorganisms play crucial roles in maintaining plant health by providing nutrients and resistance to biotic abiotic stresses. However, their functions seed germination disease remain poorly understood. In this study, we investigated the microbial community assembly features functional profiles of spermosphere endosphere microbiomes related germinated ungerminated seeds Astragalus mongholicus using amplicon shotgun metagenome sequencing techniques. Additionally, aimed elucidate relationship between beneficial through both vitro vivo pot experiments. Our findings revealed that significantly enhances diversity communities associated with seeds. This increase is driven environmental ecological niche differentiation, leading enrichment potentially probiotic bacteria such as Pseudomonas Pantoea. Conversely, Fusarium was consistently enriched The co-occurrence network patterns within presented distinct structures. Notably, exhibit more complex interconnected networks, particularly for bacterial interactions fungi. Metagenome analysis showed soil had pathogen inhibition cellulose degradation. Through a combination culture-dependent experiments, identified solani pathogen. Consistent analysis, experiments further demonstrated degradation could promote vigor. Specifically, Paenibacillus sp. enhanced A. growth. study dynamics seed-associated during confirmed role promoting suppressing pathogens degrading cellulose. offers mechanistic understanding how facilitate successful germination, highlighting potential leveraging these health.

Language: Английский

Citations

1
Depletion of protective microbiota promotes the incidence of fruit disease DOI Creative Commons
Xue Luo, Kai Sun, Haoran Li

et al.

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Language: Английский

Citations

8
Plant–pathogen interaction with root rot of Panax notoginseng as a model: Insight into pathogen pathogenesis, plant defence response and biological control DOI Creative Commons
Jianbin Li,

Mingtao Ai,

Jiae Hou

et al.

Molecular Plant Pathology, Journal Year: 2024, Volume and Issue: 25(2)

Published: Feb. 1, 2024

Abstract Plant diseases are a major threat affecting the sustainability of global agriculture. Although breeding new resistant cultivars is considered to be primary approach prevent and control plant diseases, it dependent on an in‐depth understanding plant–pathogen interactions. At present, we have interactions between model plants pathogens, such as Arabidopsis thaliana rice, but still in beginning stage for more non‐model (e.g., medicinal plants). Panax notoginseng source high‐value active ingredient triterpenoid saponins. Root rot disease P. has attracted research attention because its high destructiveness. Understanding infection stages strategies resistance mechanisms induced defence against pathogens essential support agricultural sustainable development . Here, review summarize, with root model, current knowledge interaction, feasability use microorganisms secondary metabolites sources biological agents at low cost. Finally, also discuss importance breeding, thereby providing strategy develop green agriculture plants.

Language: Английский

Citations

7
The underground world of plant disease: Rhizosphere dysbiosis reduces above‐ground plant resistance to bacterial leaf spot and alters plant transcriptome DOI Creative Commons

Toi Ketehouli,

Josephine Pasche,

Victor Hugo Buttrós

et al.

Environmental Microbiology, Journal Year: 2024, Volume and Issue: 26(7)

Published: July 1, 2024

Abstract Just as the human gut microbiome is colonized by a variety of microbes, so too rhizosphere plants. An imbalance in this microbial community, known dysbiosis, can have negative impact on plant health. This study sought to explore effect dysbiosis health tomato plants ( Solanum lycopersicum L.), using them and foliar bacterial spot pathogen Xanthomonas perforans model organisms. The rhizospheres 3‐week‐old were treated with either streptomycin or water control, then spray‐inoculated X. after 24 h. Half that both received soil transplants from uninfected donors 48 h was applied. showed 26% increase disease severity compared those did not receive antibiotic. However, transplant exhibited an intermediate level severity. antibiotic‐treated demonstrated reduced abundance rhizobacterial taxa such Cyanobacteria genus Cylindrospermum . They also down‐regulation genes related primary secondary metabolism, up‐regulation defence associated induced systemic resistance. highlights vital role beneficial microbes play resistance, even against pathogens.

Language: Английский

Citations

7
Role of Bacillus subtilis exopolymeric genes in modulating rhizosphere microbiome assembly DOI Creative Commons
Caroline Sayuri Nishisaka,

João Paulo Ventura,

Harsh P. Bais

et al.

Environmental Microbiome, Journal Year: 2024, Volume and Issue: 19(1)

Published: May 14, 2024

Abstract Background Bacillus subtilis is well known for promoting plant growth and reducing abiotic biotic stresses. Mutant gene-defective models can be created to understand important traits associated with rhizosphere fitness. This study aimed analyze the role of exopolymeric genes in modulating tomato microbiome assembly under a gradient soil diversities using B. wild-type strain UD1022 its corresponding mutant eps−TasA , which defective exopolysaccharide ( EPS ) TasA protein production. Results qPCR revealed that eps−TasA− has diminished capacity colonize roots soils diluted microbial diversity. The analysis bacterial β-diversity significant differences fungal community structures following inoculation either or strains. Verrucomicrobiota Patescibacteria, Nitrospirota phyla were more enriched than inoculation. Co-occurrence when was inoculated tomato, exhibited lower level modularity, fewer nodes, communities compared . Conclusion advances our understanding genes, are not only root colonization but also play shaping assembly. Future research should concentrate on specific genetic their implications colonization, coupled modulation. These efforts will crucial optimizing PGPR-based approaches agriculture.

Language: Английский

Citations

6