Evolution of genotypic and phenotypic diversity in multispecies biofilms DOI Open Access
Cristina I. Amador,

Sofia Zoe Moscovitz,

Lorrie Maccario

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 8, 2023

Abstract The fitness and adaptability of bacteria in microbial communities can be influenced by the interactions among community partners, as well specific spatial organisation. Here, we investigated evolutionary dynamics Bacillus thuringiensis response to presence or absence structure bacterial interspecies interactions. During evolution experiments, a distinct phenotypic variant B. frequently occurred, irrespective conditions tested: planktonic vs. biofilm monospecies mixed species. Remarkably, selection significantly favored this over its ancestor settings when coexisting with Pseudomonas defluvii brenneri , co-isolated from wastewater facility. Interestingly, evolved phenotype displayed lower biomass species biofilms, shorter generation time, lacked sporulation compared ancestor. strains displaying variation contained mutations regulator spo0A that initiates but also de-represses expression matrix determinants other Bacilli. Consistently, proteomics revealed reduced abundance TasA variant, major component species, while higher was found co-culture P. . Our results indicate within biofilms not only promote diversification select for altered traits such production. Moreover, favoured settings, suggesting facilitate co-existence. These findings could impact applications where are utilized consortia, plant growth promoters biopesticides.

Language: Английский

Root colonization by beneficial rhizobacteria DOI Creative Commons
Yunpeng Liu, Zhihui Xu, Lin Chen

et al.

FEMS Microbiology Reviews, Journal Year: 2023, Volume and Issue: 48(1)

Published: Dec. 12, 2023

Rhizosphere microbes play critical roles for plant's growth and health. Among them, the beneficial rhizobacteria have potential to be developed as biofertilizer or bioinoculants sustaining agricultural development. The efficient rhizosphere colonization of these is a prerequisite exerting their plant functions, but colonizing process underlying mechanisms not been thoroughly reviewed, especially nonsymbiotic rhizobacteria. This review systematically analyzed root compared it with that symbiotic pathogenic bacteria. also highlighted approaches improve efficiency proposed study rhizobacterial from holistic perspective microbiome under more natural conditions.

Language: Английский

Citations

49
Pyoluteorin-deficient Pseudomonas protegens improves cooperation with Bacillus velezensis, biofilm formation, co-colonizing, and reshapes rhizosphere microbiome DOI Creative Commons
Qian Zhao, Ruoyi Wang,

Yan Song

et al.

npj Biofilms and Microbiomes, Journal Year: 2024, Volume and Issue: 10(1)

Published: Dec. 11, 2024

Plant-beneficial Pseudomonas and Bacillus have been extensively studied applied in biocontrol of plant diseases. However, there is less known about their interaction within two-strain synthetic communities (SynCom). Our study revealed that protegens Pf-5 inhibits the growth several species, including velezensis. We established a combination DMW1 to elucidate interaction. In this combination, pyoluteorin conferred competitive advantage Pf-5. Noteworthy, pyoluteorin-deficient cooperated with biofilm formation, production metabolites, root colonization, tomato bacterial wilt disease control, as well cooperation beneficial bacteria rhizosphere, such spp. RNA-seq analysis RT-qPCR also proved mutant improved cell motility metabolite production. This suggests cooperative effect Bacillus–Pseudomonas consortia depends on balance pyoluteorin. finding needs be considered developing efficient SynCom sustainable agriculture.

Language: Английский

Citations

4
The extracytoplasmic sigma factor σX supports biofilm formation and increases biocontrol efficacy in Bacillus velezensis 118 DOI Creative Commons

Yanfei Cai,

Huan Tao,

Ahmed Gaballa

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 13, 2025

Plant growth promoting rhizobacteria (PGPR) offer an environmentally friendly and sustainable approach to combat pathogens enhance crop production. The biocontrol activity of PGPR depends on their ability colonize plant roots synthesize antimicrobial compounds that inhibit pathogens. However, the regulatory mechanisms underlying these processes remain unclear. In this study, we isolated characterized Bacillus velezensis 118, a soil isolate exhibits potent against Fusarium wilt banana. Deletion sigX, encoding extracytoplasmic function (ECF) sigma factor previously implicated in controlling biofilm architecture B. subtilis, reduced efficacy. 118 sigX mutant displayed formation but had only minor defect swarming motility negligible impact lipopeptide These findings highlight importance important for root colonization effectiveness spp. as agents phytopathogens.

Language: Английский

Citations

0
Improving Bacillus subtilis as Biological Chassis Performance by the CRISPR Genetic Toolkit DOI
Xingzhong Cao, Xiaojuan Wang, Ruirui Chen

et al.

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Bacillus subtilis is the model Gram-positive and industrial chassis bacterium; it has blossomed as a robust promising host for enzyme, biochemical, or bioflocculant production. However, synthetic biology metabolic engineering technologies of B. have lagged behind most widely used Saccharomyces cerevisiae Escherichia coli. CRISPR (an acronym clustered regularly interspaced short palindromic repeats) enables efficient, site-specific, programmable DNA cleavage, which revolutionized manner genome editing. In 2016, technology was first introduced into been intensely upgraded since then. this Review, we discuss recently developed key additions to toolkit design in with gene editing, transcriptional regulation, enzyme modulation. Second, advances efficient biochemicals proteins are discussed. Finally, conclude perspectives on challenges opportunities CRISPR-based biotechnology subtilis, wishing that can be comparable traditional microorganisms such E. coli S. someday soon.

Language: Английский

Citations

0
Early inoculation and bacterial community assembly in plants: A review DOI

Xing Wang,

Yuyi Li, Christopher Rensing

et al.

Microbiological Research, Journal Year: 2025, Volume and Issue: unknown, P. 128141 - 128141

Published: March 1, 2025

Language: Английский

Citations

0
Potential of Bacillus halotolerans in Mitigating Biotic and Abiotic Stresses: A Comprehensive Review DOI Creative Commons
Pelias Rafanomezantsoa, Abbas El‐Hasan, Ralf T. Voegele

et al.

Stresses, Journal Year: 2025, Volume and Issue: 5(2), P. 24 - 24

Published: March 25, 2025

Bacillus halotolerans, a halophilic bacterial species of the genus Bacillus, is emerging as biological control agent with immense potential for sustainable agriculture, particularly in extreme conditions and environmental rehabilitation. This review summarizes current state research on B. emphasizing its diverse applications biocontrol plant pathogens, growth promotion under salinity stress, nematode management, bioremediation. halotolerans utilizes several mechanisms such production siderophores phytohormones, secretion exopolysaccharides, release antifungal nematicidal compounds, which allows it to mitigate both abiotic biotic stresses various crops, including wheat, rice, date palm, tomato, others. In addition, genomic metabolomic analyses have revealed secondary metabolite that improves antagonistic growth-promoting traits. Despite significant progress, challenges remain translating laboratory results into field applications. Future should focus formulating effective bioinoculants trials maximize practical utility agriculture resilience.

Language: Английский

Citations

0
In vivo plant-bacteria interaction rather than root exudates exploitation determines the evolution of bacterial rhizosphere adaptation DOI
Nan Zhang, Zhengqi Wang, Weibing Xun

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Abstract Plants dominates niche and functional differentiation between rhizosphere bulk soil microorganisms, however, the dominant driving force directing evolution from uncompetitive root colonizers (bulk habitants) to competitive ones (rhizosphere habitants), are still being questioned. In present evolutionary experiment, transferred growth of Bacillus amyloliquefaciens DSM7T (a weak colonizer) in exudates in vitro improved metabolism competitiveness but failed enhance its colonization capacity. Conversely, vivo plant significantly promoted bacterial association, which is attributed evolved biofilm formation indole-3-acetic acid production. Genome sequencing also identified hotspot mutations relevant adaptation.

Language: Английский

Citations

0
Taxonomy of Pseudomonas spp. determines interactions with Bacillus subtilis DOI Creative Commons
Mark Lyng,

Birta Þórisdóttir,

Sigrún H. Sveinsdóttir

et al.

mSystems, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

ABSTRACT Bacilli and pseudomonads are among the most well-studied microorganisms commonly found in soil frequently co-isolated. Isolates from these two genera used as plant beneficial microorganisms; therefore, their interaction rhizosphere is relevant for agricultural applications. Despite this, no systematic approach has been employed to assess coexistence of members genera. Here, we screened 720 fluorescent isolates effects on Bacillus subtilis pellicle formation types media a predictor outcome Pseudomonas taxonomy. Interactions were context-dependent, both medium composition culture conditions strongly influenced interactions. Negative interactions associated with capeferrum , entomophila protegens 2,4-diacetylphloroglucinol was confirmed strong (but not exclusive) inhibitor B. . Non-inhibiting strains closely related trivialis lini Using such non-inhibiting isolate, P9_31, which increased demonstrated that species spatially segregated cocultures. Our study first one propose an overall negative pairwise between pseudomonads; hence, cocultures comprising groups likely require additional coexistence. IMPORTANCE There interest microbial ecology field predict microorganisms, whether will promote each other’s growth or compete resources. Numerous studies have performed based surveying available literature testing phylogenetically diverse sets synthetic communities. high throughput screening using isolates, impact biofilm tested. The aim determine majority inhibit biofilms co-cultures. This reports taxonomy good outcome, only minority establishment.

Language: Английский

Citations

3
Rooting for success: Evolutionary enhancement of Bacillus for superior plant colonization DOI Creative Commons
Vincent Charron‐Lamoureux,

Sandrine Lebel‐Beaucage,

Maude Pomerleau

et al.

Microbial Biotechnology, Journal Year: 2024, Volume and Issue: 17(9)

Published: Aug. 30, 2024

Many strains from the Bacillus subtilis species complex exert strong plant growth-promoting activities. However, their efficacy in relevant conditions is variable, due part to inability establish a interaction with roots stressful environmental conditions. Adaptative laboratory evolution (ALE) powerful tool generate novel traits of interest. evolved isolates, stemming ALE performed plants, possess stronger root colonization capacity. An in-depth analysis these isolates also allowed identification key features influencing roots. many variables can influence outcome assays, and thus, caution should be taken when designing destined better colonizers.

Language: Английский

Citations

2
Exploring overlooked growth-promoting mechanisms by plant-associated bacteria DOI Creative Commons
Antoine Danchin

Deleted Journal, Journal Year: 2024, Volume and Issue: 1(1)

Published: Jan. 1, 2024

Abstract Agriculture-oriented microbiome studies try to develop microbiota beneficial their plant hosts. This positive goal extends the soil quality driving growth and disease resistance. In research aimed at identifying causes of this action, a common interpretation is that microbes will synthesize metabolites useful view assumes important microbial are exported for use by Yet, seems unlikely essential metabolites, without counterpart imported from plants, as corresponding syntheses would often involve consumption resources explicit benefit microbes. Illustrating function with example Bacilli Subtilis clade, we emphasize here most direct access contents cells through cell lysis, phenomenon linked process sporulation. also releases macromolecules digested in environment, releasing key such queuine, an base analog present anticodon some transfer RNAs. overlooked importance lysis could be major cause ubiquitous presence bacteriophages microbiota.

Language: Английский

Citations

1