Wychimicins E and F from a rare actinomycete of the genus Cryptosporangium

The Journal of Antibiotics, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 3, 2024

Language: Английский

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Microbe Profile: Pseudonocardia: antibiotics for every niche

Microbiology, Journal Year: 2024, Volume and Issue: 170(9)

Published: Sept. 19, 2024

species comprise a genus of filamentous, sporulating bacteria belonging to the phylum Actinomycetota, formerly Actinobacteria. They are found in marine and freshwater sediments soils associated with animals, insects, plants. To date, they have mostly been studied because their mutually beneficial symbiosis fungus-growing ants tribe

Language: Английский

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1-Acetyl-β-Carboline from a Jeju Gotjawal Strain Lentzea sp. JNUCC 0626 and Its Melanogenic Stimulating Activity in B16F10 Melanoma Cells

Molecules, Journal Year: 2024, Volume and Issue: 29(19), P. 4586 - 4586

Published: Sept. 27, 2024

The genus Lentzea is a prolific source of bioactive and structurally diverse secondary metabolites. We isolated novel strain, sp. JNUCC 0626, from Hwasun Gotjawal on Jeju Island, Korea. Based 16S rRNA partial gene sequence analysis, strain 0626 closely related to isolaginshaensis NX62 (99.41% similarity), pudingi DHS C021 (99.31%), cavernae SYSU K10001 (99.26%). From the fermentation broth we 1-acetyl-β-carboline, whose structure was established using IR, HR-ESI-MS, 1D- 2D-NMR techniques. 1-acetyl-β-carboline found activate melanogenesis in mouse B16F10 cells without cytotoxicity at concentrations up 50 μM. At this concentration, compound increased melanin content by 27.44% tyrosinase activity 240.64% compared control, upregulating key melanogenic enzymes, including tyrosinase, TRP-1, TRP-2, microphthalmia-associated transcription factor (MITF), central regulator melanogenesis. In addition, significantly inhibited ERK phosphorylation, reducing it 20.79% concentration 12.5 μM 25.63% 25 This inhibition supports hypothesis that enhances synthesis MITF enzymes via signaling pathway. study aimed isolate identify discovered Gotjawal, evaluate its effect production melanoma cells. Skin irritation tests 32 subjects confirmed safety for topical use, findings suggest which cytotoxicity, holds promise as therapeutic agent hypopigmentation-related conditions or cosmetic ingredient.

Language: Английский

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A modular toolkit for environmentalRhodococcus, Gordonia, andNocardiaenables complex metabolic manipulation

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 22, 2024

ABSTRACT Soil-dwelling Actinomycetes are a diverse and ubiquitous component of the global microbiome, but largely lack genetic tools comparable to those available in model species such as E. coli or Pseudomonas putida , posing fundamental barrier their characterization utilization hosts for biotechnology. To address this, we have developed modular plasmid assembly framework along with series control elements previously genetically intractable Gram-positive environmental isolate Rhodococcus ruber C208 demonstrate conserved functionality isolates Rhodococcus, Nocardia Gordonia . This toolkit encompasses Mycobacteriale origins replication, broad-host range antibiotic resistance markers, transcriptional translational elements, fluorescent reporters, tetracycline-inducible system, counter-selectable marker. We use this interrogate carotenoid biosynthesis pathway erythropolis N9T-4, weakly carotenogenic engineer higher flux towards keto-carotenoid canthaxanthin. work establishes several new Mycobacteriales provides synthetic biology support design complex circuits these species. IMPORTANCE Actinomycetes, particularly Mycobacteriales, include both sustainable biomanufacturing emerging opportunistic pathogens. three abundant genera flexible metabolisms untapped potential natural product discovery. Among these, was shown degrade polyethylene, paraffinivorans can assimilate carbon from solid hydrocarbons, neocaledoniensis (and many other ) possesses dual isoprenoid pathways. Many accumulate high levels pigments, indicative highly active pathways which may be harnessed fermentation terpenes commodity isoprenoids. Modular toolkits proven valuable applied research organisms, lacking most Actinomycetes. Our suite DNA were broad facilitate rapid prototyping constructs organisms.

Language: Английский

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Editorial overview: emerging avenues in antimicrobial research

Current Opinion in Microbiology, Journal Year: 2024, Volume and Issue: 81, P. 102522 - 102522

Published: Aug. 7, 2024

Language: Английский

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