Synergistic Action of Rutin‐Coated Zinc Oxide Nanoparticles: Targeting Biofilm Formation Receptors of Dental Pathogens and Modulating Apoptosis Genes for Enhanced Oral Anticancer Activity DOI
Mohammed Rafi Shaik, Mohankumar Ramasamy, Divya Jain

et al.

Journal of Biochemical and Molecular Toxicology, Journal Year: 2024, Volume and Issue: 38(11)

Published: Oct. 29, 2024

ABSTRACT Oral diseases are often associated with bacterial and fungal pathogens such as Staphylococcus aureus , Streptococcus mutans Enterococcus faecalis Candida albicans . This research explored a novel approach to addressing these by synthesizing zinc oxide nanoparticles (ZnO NPs) coated rutin (RT), plant‐derived compound. The synthesized ZnO‐RT NPs were comprehensively characterized using UV‐Vis spectrophotometer, SEM, EDAX techniques confirm their structural composition. antioxidant potential was assessed through free radical scavenging assays. Additionally, the antimicrobial activity of evaluated zone inhibition assay against oral pathogens. Molecular docking studies Autodock tool performed elucidate interactions between RT receptors findings demonstrated that exhibited robust activity. Furthermore, they showed significant minimal inhibitory concentration 40 μg/mL also displayed dose‐dependent anticancer effects on human cancer cells at concentrations 10, 20, 40, 80 μg/mL. Mechanistic insights into KB revealed upregulation apoptotic genes. study underscores promising for dental applications due strong antioxidant, anticancer, properties. These offer hopeful prospect pathogen challenges enhancing overall health.

Language: Английский

Innovative Approaches to Combating Dental Biofilms: Nanotechnology and its Applications DOI

Akankshya Dash,

Chinnasamy Ragavendran

Biocatalysis and Agricultural Biotechnology, Journal Year: 2024, Volume and Issue: 61, P. 103406 - 103406

Published: Oct. 1, 2024

Language: Английский

Citations

3
Decoding gene expression dynamics in planktonic and biofilm cells of Streptococcus mutans: regulation and role of mutanofactin genes in biofilm formation DOI Creative Commons
Muhammad Afzal, Miguel Carda‐Diéguez, Susanne Bloch

et al.

Frontiers in Oral Health, Journal Year: 2025, Volume and Issue: 6

Published: Jan. 17, 2025

Dental caries is the most prevalent chronic infectious disease globally, with Streptococcus mutans recognized as a primary causative agent due to its acidogenicity and robust biofilm-forming ability. In S. biofilm formation, role of autoinducers has been extensively studied, while influence other small molecules remains largely unexplored. Mutanofactins, class polyketide/non-ribosomal lipopeptide secondary metabolites, are emerging potential modulators development. Transcriptomic analysis was conducted examine gene expression patterns in NMT4863 across distinct growth phases lifestyles, aiming identify metabolic factors influencing formation. profiles were compared between cells early-, mid-, late-exponential-, stationary phase, well planktonic cells. Differentially expressed genes identified, pathway analyses revealed significant alterations key regulatory pathways. Specifically, biosynthetic mutanofactin cluster analyzed via quantitative real-time polymerase chain reaction. Several operons differentially tested phases, 1,095 showing differential stationary-phase, Pathway changes ascorbate metabolism, carbohydrate utilization transport systems, lipoic acid bacterial toxin pathways, two-component metabolite biosynthesis. Notably, muf cluster, elevated early exponential-phase relative stationary-phase Additionally, mufCDEFGHIJ identified components single transcriptional unit (muf operon). MufC, regulator TetR/AcrR-family, acts positive operon strain NMT4863. Bioinformatic pinpointed 20-bp sequence promoter region (5'-AAATGAGCTATAATTCATTT-3'). Interestingly, found be significantly downregulated This study provides insights into dynamics that drive formation NMT4863, particular emphasis on operon. governed by TetR/AcrR-family MufC plays central development, offering novel perspective molecular basis resilience.

Language: Английский

Citations

0
Polymicrobial interactions of Helicobacter pylori and its role in the process of oral diseases DOI Creative Commons

Y.F. Fan,

Xi Chen, Tiantian Shan

et al.

Journal of Oral Microbiology, Journal Year: 2025, Volume and Issue: 17(1)

Published: Feb. 25, 2025

Objective Helicobacter pylori (H. pylori) infection affects approximately 50% of the global population. The predominant route H. transmission is through oral pathway, making cavity highly significant in its infection. This review focuses on relationship between and diseases, influence microbiota, potential mechanisms involving certain pathogens.

Language: Английский

Citations

0
Ureases in nature: Multifaceted roles and implications for plant and human health - A review DOI
Majid Khan, Bo Zhang, Han Zhang

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 306, P. 141702 - 141702

Published: March 3, 2025

Language: Английский

Citations

0
Harnessing the functional role of polymeric nanocomposite in dental caries disease: A comprehensive review DOI Creative Commons

T. Selva Kumar,

Rajkuberan Chandrasekaran

Hybrid Advances, Journal Year: 2025, Volume and Issue: unknown, P. 100433 - 100433

Published: March 1, 2025

Language: Английский

Citations

0
Bacteriophage-Based Therapies in Oral Cancer: A New Frontier in Oncology DOI Creative Commons

Vishnu Priya Panneerselvam,

Leela Kagithakara Vajravelu,

Rahul Harikumar Lathakumari

et al.

Cancer Pathogenesis and Therapy, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Investigation of Antimicrobial and Anti-Adherent Properties of Fabricated Cobalt-Chromium alloy modified with TiO2 coating DOI Creative Commons

Loghman Ghahremani,

Amirmahdi Azizi Majdian,

Masoud Sarraf

et al.

Results in Surfaces and Interfaces, Journal Year: 2025, Volume and Issue: 19, P. 100530 - 100530

Published: April 18, 2025

Language: Английский

Citations

0
Exploring the Potential of Halotolerant Actinomycetes from Rann of Kutch, India: A Study on the Synthesis, Characterization, and Biomedical Applications of Silver Nanoparticles DOI Creative Commons

Paras Dayma,

Nisha Choudhary, Daoud Ali

et al.

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(6), P. 743 - 743

Published: June 6, 2024

A tremendous increase in the green synthesis of metallic nanoparticles has been noticed last decades, which is due to their unique properties at nano dimension. The present research work deals with mediated by actinomycete Streptomyces tendae silver (AgNPs), isolated from Little and Greater Rann Kutch, India. confirmation formation AgNPs actinomycetes was carried out using a UV-Vis spectrophotometer where an absorbance peak obtained 420 nm. X-ray diffraction pattern demonstrated five characteristic peaks indexed lattice plane (111), (200), (231), (222), (220). Fourier transform infrared showed typical bands 531 1635, 2111, 3328 cm−1. Scanning electron microscopy shows that spherical-shaped particles have diameters range 40 90 particle size analysis displayed mean aqueous medium, about 55 nm (±27 nm), bearing negative charge on surfaces. potential S. tendae-mediated synthesized evaluated for antimicrobial, anti-methicillin-resistant Staphylococcus aureus (MRSA), anti-biofilm, anti-oxidant activity. maximum inhibitory effect observed against Pseudomonas aeruginosa (8 µg/mL), followed Escherichia coli Aspergillus niger (32 Candida albicans (64 whereas Bacillus subtilis (128 µg/mL) (256 were much less sensitive AgNPs. biosynthesized activity MRSA, free radical scavenging dosage 25 200 µg/mL. combination ampicillin inhibition development biofilm Streptococcus pneumoniae 98% 83%, respectively. also successfully coated surface cotton prepare antimicrobial surgical cotton, action (15 mm) (12 mm). integrates microbiology, nanotechnology, biomedical science formulate environmentally friendly materials halotolerant actinomycetes, evolving nanotechnology field. Moreover, this study broadens understanding opens possibilities formulating new products therapies.

Language: Английский

Citations

3
Biofilm formation of Streptococcus mutans, Streptococcus sanguinis, Staphylococcus epidermidis, Staphylococcus aureus, Lactobacillus casei, and Candida Albicans on 5 thermoform and 3D printed orthodontic clear aligner and retainer materials at 3 time points: an in vitro study DOI Creative Commons

Mehrnaz Moradinezhad,

Effat Abbasi Montazeri, Alireza Hashemi Ashtiani

et al.

BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: Sept. 18, 2024

Orthodontic clear aligners and retainers have numerous advantages that is making them ever increasingly popular. However, they might, similar to any other oral appliance, contribute biofilm formation finally dental caries or white spot lesions gingival inflammations. The literature on orthodontic appliances very scarce limited a few microorganisms materials. Therefore, this experimental study evaluated the 5 thermoformed 3D printed CAD/CAM in 3 intervals.

Language: Английский

Citations

3
Tetrahedral framework nucleic acid–based small‐molecule inhibitor delivery for ecological prevention of biofilm DOI Creative Commons
Yuhao Liu,

Kechen Li,

Weijie Zhuang

et al.

Cell Proliferation, Journal Year: 2024, Volume and Issue: 57(9)

Published: May 29, 2024

Biofilm formation constitutes the primary cause of various chronic infections, such as wound gastrointestinal inflammation and dental caries. While preliminary achievement biofilm inhibition is possible, challenge lies in difficulty eliminating bactericidal effects current drugs that lead to microbiota imbalance. This study, utilizing vitro vivo models caries, aims efficiently inhibit without inducing effects, even against pathogenic bacteria. The tetrahedral framework nucleic acid (tFNA) was employed a delivery vector for small-molecule inhibitor (smI) specifically targeting activity glucosyltransferases C (GtfC). It observed tFNA loaded smI small-groove binding manner, transferring it into Streptococcus mutans, thereby inhibiting GtfC extracellular polymeric substances compromising bacterial survival. Furthermore, smI-loaded demonstrated reduction severity caries adversely affecting oral microbial diversity exhibited desirable topical systemic biosafety. study emphasizes concept 'ecological prevention biofilm', which anticipated advance optimization strategies clinical application DNA nanocarrier-based drug formulations.

Language: Английский

Citations

2