Natural Products Produced by the Species of Bacillus cereus Group: Recent Updates DOI
Uğur Azizoğlu, Leandris Argentel‐Martínez, Ofelda Peñuelas‐Rubio

et al.

Journal of Basic Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 21, 2024

ABSTRACT Bacillus cereus group produces diverse antimicrobial compounds through different metabolic pathways, including amino acid‐based compounds, sugar derivatives, volatile and miscellaneous compounds. These exhibit antibacterial antifungal activities against various plant pathogens, promoting growth enhancing tolerance to abiotic stresses. They also nematicidal nematodes antagonistic effects pathogens in aquatic animals, inducing immune responses. Moreover, B. bacteria play a significant role bioremediation by breaking down or neutralizing environmental pollutants, such as plastics, petroleum products, heavy metals, insecticides. produce enzymes like laccases, lipases, proteases, oxidases, contributing the degradation of these pollutants. In food industry, they can cause poisoning due their production enterotoxins. However, are utilized industrial applications, producing environmentally friendly bio‐based materials, biofertilizers, nanoparticles. Notably, transforms selenite into selenium nanoparticles, which have health benefits, cancer prevention. summary, applications agriculture, bioremediation, medicine, sustainable eco‐friendly solutions across multiple fields. this review, we revised characteristics every species; highlighted more important secreted species The aim is explain available secondary metabolites classify from group, increasing knowledge about taxonomy group.

Language: Английский

Whole genome sequencing and analysis of Bacillus sp. TTMP2, a tetramethylpyrazine-producing bacterium DOI

Shanling Gan,

Lingru Ruan,

Xinyi Xu

et al.

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: July 29, 2024

Language: Английский

Citations

5
Enhancing Bacillus cereus antibacterial ability through improved cofactor supply DOI Creative Commons

Yinbiao Xu,

Jiaxuan Wu, Tingting Yuan

et al.

Microbial Cell Factories, Journal Year: 2025, Volume and Issue: 24(1)

Published: March 6, 2025

Bacillus cereus 0-9 is a biocontrol microorganism that antagonizes Gram-positive bacteria and pathogenic fungi, such as Staphylococcus aureus Gaeumannomyces graminis, through the secretion of antimicrobial peptides. However, its low antibacterial activity limits application. In this study, significant enhancement in against S. was achieved by overexpressing glucose dehydrogenase from subtilis (BsGDH) B. 0-9, expanding 6.98 to 11.59 U/mL, representing 66% improvement. To further improve capability, we aimed catalytic efficiency BsGDH screening 11 low-conserved residues protein's second-shell via conservation analysis molecular docking. Following three rounds saturation mutagenesis, specific enzyme Kcat/Km value variant N97F/N192S/E198G reached 289.74 U/mg 4.95 µM⁻¹·min⁻¹, 5.66 11.38 times greater than wild-type BsGDH, respectively. Molecular docking suggested Gly94, Gly14, Ile191 form triangular region enhancing substrate affinity enzymatic activity. Furthermore, Root Mean Square Fluctuation dynamics showed conformational changes five regions mutants (α2 helix, α3 α5 helix + β4 sheet, α8 β5 α13-14 helix), increasing flexibility active pocket. Ultimately, expressing 22.79 2.26 higher 0-9. This study offers promising candidate for NAD(P)+ metabolic cycling peptide synthesis cells industrial applications.

Language: Английский

Citations

0
Do Organic Amendments Foster Only Beneficial Bacteria in Agroecosystems?: The Case of Bacillus paranthracis TSO55 DOI Creative Commons
Ixchel Campos-Avelar, Amelia C. Montoya-Martínez,

Alina Escalante-Beltrán

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(7), P. 1019 - 1019

Published: March 25, 2025

Bacterial strain TSO55 was isolated from a commercial field of wheat (Triticum turgidum L. subsp. durum), under organic amendments, located in the Yaqui Valley, Mexico. Morphological and microscopical characterization showed off-white irregular colonies Gram-positive bacillus, respectively. The draft genome sequence this revealed genomic size 5,489,151 bp, with G + C content 35.21%, N50 value 245,934 L50 8, 85 contigs. Taxonomic affiliation that belongs to Bacillus paranthracis, reported as an emergent human pathogen. Genome annotation identified 5743 5587 coding DNA sequences (CDSs), respectively, highlighting genes associated indole production, phosphate potassium solubilization, iron acquisition. Further silico analysis indicated presence three CDSs related pathogenicity islands high pathogenic potential (77%), well multiple gene clusters antibiotic resistance. vitro evaluation plant growth promotion traits negative for production it positive but low (18%) siderophore production. biosynthetic cluster bacillibactin (siderophore) biosynthesis confirmed. Antifungal bioactivity evaluated against fungi (Alternaria alternata TF17, Bipolaris sorokiniana TPQ3, Fusarium incarnatum TF14) minimal fungal inhibition. An susceptibility assay resistance six antibiotics evaluated, up concentration 20 µg/mL. beta hemolysis result on blood agar reinforced TSO55's potential. Inoculation B. paranthracis seedlings resulted significant decrease root length (-8.4%), total height (-4.2%), dry weight (-18.6%), stem (-11.1%), (-15.2%) compared control (uninoculated) treatment. This work highlights importance analyzing microbiological safety amendments before application. Comprehensive genome-based taxonomic bioprospecting microbial species introduced soil by agricultural practices any inoculant will prevent introduction dangerous non-beneficial crops, which affect sustainability generate health risks plants humans.

Language: Английский

Citations

0
Mycoidesin, a novel lantibiotic, exhibits potent bacteriostatic activity against Listeria monocytogenes and effectively controls its growth in beef DOI Creative Commons
Fei Zhang,

Jiajia Ding,

Shu Liu

et al.

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

ABSTRACT Listeria monocytogenes can cause severe listeriosis, with the consumption of contaminated food being an important route its transmission. Biopreservatives be used for prevention and control L. in food. In this study, we identified a novel lantibiotic, mycoidesin, potent bacteriostatic activity against . It exhibited 4- to 16-fold higher strains than nisin A. Analysis mode action mycoidesin revealed that it exerted ATCC 19111 at low high concentrations (1×–32× MIC, 0.39–12.5 µM). blocked cell wall synthesis by binding Lipid II inhibiting growth For other sensitive strains, such as Bacillus cereus CMCC 63301, effect concentration (1× 1.56 µM) via same mechanism, whereas bactericidal (2×–8× 3.13–12.5 µM), which damage membrane death. The stability test showed had increased compared Additionally, cytotoxic hemolytic activity. Furthermore, effectively inhibited beef delayed decline quality. Our study demonstrates potential biopreservative prevent contamination improve safety meat products industry. IMPORTANCE This aimed identify highly effective, stable, safe natural bacteriocin preservatives anti- We isolated class more efficient applied preservative, Mycoidesin also favorable biosafety. Moreover, could controlling beef, demonstrating -related agricultural industries.

Language: Английский

Citations

0
Valorization of Sugarcane Bagasse for Co-Production of Poly(3-hydroxybutyrate) and Bacteriocin Using Bacillus cereus Strain S356 DOI Open Access

Sunisa Khamberk,

Sutticha Na-Ranong Thammasittirong, Anon Thammasittirong

et al.

Polymers, Journal Year: 2024, Volume and Issue: 16(14), P. 2015 - 2015

Published: July 15, 2024

Poly(3-hydroxybutyrate) (P(3HB)) is an attractive biodegradable plastic alternative to petroleum-based plastic. However, the cost of microbial-based bioplastic production mainly lies in cultivation medium. In this study, we screened isolates capable synthesizing P(3HB) using sugarcane bagasse (SCB) waste as a carbon source from 79 Bacillus that had previously shown commercial The results revealed isolate S356, identified cereus 16S rDNA and gyrB gene analysis, highest accumulation. yield (5.16 g/L, 85.3% dry cell weight) was achieved by cultivating B. S356 optimal medium with 1.5% total reducing sugar SCB hydrolysate 0.25% yeast extract nitrogen source. Transmission electron microscopy analysis showed accumulation approximately 3–5 granules each cell. Proton nuclear magnetic resonance spectroscopy Fourier transform infrared analyses confirmed polymer extracted P(3HB). Notably, during for production, also secreted bacteriocin, which high antibacterial activity against same species (Bacillus cereus). Overall, work demonstrated possibility co-producing eco-friendly bacteriocin renewable resources potential S356.

Language: Английский

Citations

1
First insights into the prevalence, genetic characteristics, and pathogenicity of Bacillus cereus from generations worldwide DOI Creative Commons

Cuihong Tong,

Danyu Xiao,

Qi Li

et al.

mSphere, Journal Year: 2024, Volume and Issue: 9(11)

Published: Oct. 23, 2024

ABSTRACT Bacillus cereus , a global threat, is one of the major causes toxin-induced foodborne diseases. However, comprehensive assessment prevalence and characteristics B. worldwide still lacking. Here, we applied whole-genome sequence analysis to 191 collected in Africa, America, Asia, Europe, Oceania from 1900s 2022, finding that CC142 dominated clonal complex. The results provided direct evidence could spread through food chain intercontinentally. different generations showed coherence antibiotic-resistant gene virulence biofilm profiles, although with high genomic heterogeneity. BCI-BCII-vanZF-fosB profiles genes were detected at rates, emphasized would pose serious challenge public health clinical treatment. IMPORTANCE This study first prevalence, genetic characteristics, pathogenicity 2022 using analysis. Moreover, revealed close evolutionary relationship between isolates sources. potential pathogenicity,

Language: Английский

Citations

1
Entomopathogenic Bacillus cereus impairs the fitness of the spotted‐wing drosophila, Drosophila suzukii DOI

Nana He,

Shaojie Zhou,

Chuanming Zhou

et al.

Insect Science, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 21, 2024

Abstract Drosophila suzukii is a notorious pest which causes devastating damage to thin‐skinned fruits, and the larvae feed on fruit, causing extensive agricultural economic loss. The current application of insecticides manage this results in serious resistance environmental hazards, so an alternative strategy for D. biocontrol urgently needed. Here, we reported that entomopathogenic Bacillus cereus has potential . We isolated identified bacterial strain, B. H1, was detrimental fitness both progenies parents. robustly repelled depositing eggs halves with metabolites H1. Both males females were susceptible H1 significantly arrested larval development at least 40% lethal larvae. median time (LT50) challenged 3 2 d, respectively. Moreover, disrupted intestinal integrity pH value resulted increase load guts hemolymph. Mechanistically, infection led activation dual oxidase (DUOX)‐ROS‐Jun N‐terminal kinase (JNK) pathway. findings showed entomopathogen could potentially act as biological control agent against , advancing fundamental concepts integrated management programs

Language: Английский

Citations

0
Characterization of newly isolated Bacillus cereus D3 Co-producing α-amylase and protease and its application in food raw materials DOI
Shuang Wang,

Hua Bai,

Zongmin Wang

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: 62, P. 105255 - 105255

Published: Oct. 10, 2024

Language: Английский

Citations

0
Natural Products Produced by the Species of Bacillus cereus Group: Recent Updates DOI
Uğur Azizoğlu, Leandris Argentel‐Martínez, Ofelda Peñuelas‐Rubio

et al.

Journal of Basic Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 21, 2024

ABSTRACT Bacillus cereus group produces diverse antimicrobial compounds through different metabolic pathways, including amino acid‐based compounds, sugar derivatives, volatile and miscellaneous compounds. These exhibit antibacterial antifungal activities against various plant pathogens, promoting growth enhancing tolerance to abiotic stresses. They also nematicidal nematodes antagonistic effects pathogens in aquatic animals, inducing immune responses. Moreover, B. bacteria play a significant role bioremediation by breaking down or neutralizing environmental pollutants, such as plastics, petroleum products, heavy metals, insecticides. produce enzymes like laccases, lipases, proteases, oxidases, contributing the degradation of these pollutants. In food industry, they can cause poisoning due their production enterotoxins. However, are utilized industrial applications, producing environmentally friendly bio‐based materials, biofertilizers, nanoparticles. Notably, transforms selenite into selenium nanoparticles, which have health benefits, cancer prevention. summary, applications agriculture, bioremediation, medicine, sustainable eco‐friendly solutions across multiple fields. this review, we revised characteristics every species; highlighted more important secreted species The aim is explain available secondary metabolites classify from group, increasing knowledge about taxonomy group.

Language: Английский

Citations

0