Studies on the ssDNA-Based Biosensor Regeneration and Miniaturization for Electrochemical Detection of miRNAs DOI Creative Commons

Izabela Zaras,

Marta Sokal,

Marta Jarczewska

et al.

Journal of The Electrochemical Society, Journal Year: 2024, Volume and Issue: 171(11), P. 117520 - 117520

Published: Nov. 1, 2024

One of the most significant disadvantages biosensing systems is limited possibility their regeneration, which only allows for single use detection targets. The reduction biosensor fabrication cost could thus be achieved by elaboration protocol providing highest recovery sensing layer response. A further drop production expenses yield miniaturization as it leads to consumption chemicals required receptor formation well execution measurements. To address above-mentioned challenges, we aimed find adequate method regeneration single-stranded DNA-based layers specific miRNA 141 molecule elevated concentration might refer progression cancer. studies indicated that 5 min. incubation ssDNA-modified electrode in 4 M urea provided response towards among all tested procedures. Furthermore, ssDNA immobilization on was shown. This enabled within 0.1 nM–1 μM range with high selectivity. Moreover, elaborated miniaturized transducers were distinguished sufficient stability after 24 h storage 20 mM PBS and also regenerated using urea.

Language: Английский

Engineering metal-organic frameworks-based nanozymes for enhanced biomimetic catalytic sensing DOI
Zhong Wei Jiang, Xue Gong, Yi Wang

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 178, P. 117862 - 117862

Published: July 10, 2024

Language: Английский

Citations

19
Cocatalysis of catalytic hairpin assembly and ternary heterojunction Bi2S3@MoS2@Bi2MoO6 promotes ultra-sensitive electrochemical of detection MiRNA-21 DOI
Ruizhuo Ouyang, Yuanhui Ma, Lan Jiang

et al.

Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(2)

Published: Jan. 31, 2025

Language: Английский

Citations

0
Preparation and properties of UiO-66 based hybrid materials via surface initiated metal-free ATRP DOI Open Access
Mei-Mei Yang,

Yanhua Wang,

Hongye Li

et al.

European Polymer Journal, Journal Year: 2023, Volume and Issue: 202, P. 112612 - 112612

Published: Nov. 20, 2023

Language: Английский

Citations

6
Studies on the ssDNA-Based Biosensor Regeneration and Miniaturization for Electrochemical Detection of miRNAs DOI Creative Commons

Izabela Zaras,

Marta Sokal,

Marta Jarczewska

et al.

Journal of The Electrochemical Society, Journal Year: 2024, Volume and Issue: 171(11), P. 117520 - 117520

Published: Nov. 1, 2024

One of the most significant disadvantages biosensing systems is limited possibility their regeneration, which only allows for single use detection targets. The reduction biosensor fabrication cost could thus be achieved by elaboration protocol providing highest recovery sensing layer response. A further drop production expenses yield miniaturization as it leads to consumption chemicals required receptor formation well execution measurements. To address above-mentioned challenges, we aimed find adequate method regeneration single-stranded DNA-based layers specific miRNA 141 molecule elevated concentration might refer progression cancer. studies indicated that 5 min. incubation ssDNA-modified electrode in 4 M urea provided response towards among all tested procedures. Furthermore, ssDNA immobilization on was shown. This enabled within 0.1 nM–1 μM range with high selectivity. Moreover, elaborated miniaturized transducers were distinguished sufficient stability after 24 h storage 20 mM PBS and also regenerated using urea.

Language: Английский

Citations

0