Histone demethylase inhibitor KDM5-C70 regulates metabolomic and lipidomic programming during an astrocyte differentiation of rat neural stem cell

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 13, 2025

Abstract Lysine-specific histone demethylase (KDM) 5 inhibition by KDM5-C70 induces astrocytogenesis and highlights the importance of modulation methylation in cell fate specification. This study investigated role inhibitor modulating metabolic lipidomic landscape during astrocyte differentiation rat neural stem cells (NSCs). Using chemical derivatisation combined with gas chromatography-mass spectrometry, 42 metabolites were detected, indicating potential regulation phospholipid metabolism. Subsequent analysis, employing reverse-phase liquid chromatography high-resolution quadrupole time-of-flight mass identified 180 lipid species 9 subclasses. Integrative analysis revealed that promoted through epigenetic changes linked to attenuation phosphatidylethanolamine (PE) biosynthesis pathways. The reduced expression transcripts related PE highlighted significance pathway influencing decisions. These quantitative metabolomic analyses not only advance our understanding NSC but also lay groundwork for therapeutic strategies targeting pathways neurodegenerative diseases injuries.

Language: Английский

Quantification of relevant metabolites in apoptotic bodies from HK-2 cells by targeted metabolomics based on liquid chromatography-tandem mass spectrometry

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1329, P. 343190 - 343190

Published: Sept. 2, 2024

Language: Английский

Inhibitory effect of Lonicera japonica flos on Streptococcus mutans biofilm and mechanism exploration through metabolomic and transcriptomic analyses

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: July 3, 2024

Introduction Streptococcus mutans was the primary pathogenic organism responsible for dental caries. Lonicera japonica flos (LJF) is a traditional herb in Asia and Europe consumed as tea beverage thousands of years. Methods The inhibitory effect mechanism LJF on biofilm formation by S. investigated. active extracts were validated their activity examining changes surface properties such adherence, hydrophobicity, auto-aggregation abilities, exopolysaccharides (EPS) production, including water-soluble glucan water-insoluble glucan. Results discussion primarily inhibited through reduction EPS resulting alterations cell characteristics growth retardation cycles. Integrated transcriptomic untargeted metabolomics analyses revealed that production modulated two-component systems (TCS), quorum sensing (QS), phosphotransferase system (PTS) pathways under stress conditions. histidine kinase VicK identified an important target protein, caused its dysregulated expression blocked autoinducer II (AI-2). This led to inhibition response regulator transcriptional factors, down-regulated glycosyltransferase (Gtf) activity, decreased glucans (WIG) (WSG). first exploration , providing theoretical basis application functional food, oral health care, related areas.

Language: Английский