Simultaneously quantifying a novel five-component anti- migraine formulation containing ergotamine, propyphenazone, caffeine, camylofin, and mecloxamine using UV spectrophotometry and chemometric models
BMC Chemistry,
Journal Year:
2024,
Volume and Issue:
18(1)
Published: Nov. 20, 2024
This
study
presents
a
new
method
for
simultaneously
quantifying
complex
anti-migraine
formulation
containing
five
components
(ergotamine,
propyphenazone,
caffeine,
camylofin,
and
mecloxamine)
using
UV
spectrophotometry
chemometric
models.
The
analytical
challenges
due
to
the
wide
variation
in
component
concentrations
(ERG:
PRO:
CAF:
CAM:
MEC
ratio
of
0.075:20:8:5:4)
highly
overlapping
spectra.
To
create
comprehensive
validation
dataset,
Kennard-Stone
Clustering
Algorithm
was
used
address
limitations
arbitrary
data
partitioning
methods.
Three
different
models
were
evaluated:
Classical
Least
Squares
(CLS),
Partial
(PLS),
Multivariate
Curve
Resolution-Alternating
(MCR-ALS).
Among
these,
MCR-ALS
demonstrated
excellent
performance,
achieving
recovery
values
98–102%
all
components,
accompanied
by
minimal
root
mean
square
errors
calibration
(0.072–0.378)
prediction
(0.077–0.404).
Moreover,
model
exhibited
high
accuracy,
with
relative
ranging
from
1.936
3.121%,
bias-corrected
between
0.074
0.389,
good
sensitivity
(0.2097–1.2898
μg
mL−1)
components.
Elliptical
Joint
Confidence
Region
analysis
further
confirmed
predictive
performance
models,
consistently
showing
smallest
ellipses
closest
ideal
point
(slope
=
1,
intercept
0)
most
analytes,
indicating
superior
accuracy
precision.
approach's
sustainability
rigorously
assessed
six
advanced
metrics,
validating
its
environmental
friendliness,
economic
viability,
practical
application.
approach
effectively
resolves
pharmaceutical
formulations,
contributing
sustainable
development
objectives
quality
control
processes.
Language: Английский
Development of HPLC-UV method for determination of Nilotinib in spiked human plasma with greenness assessment
Microchemical Journal,
Journal Year:
2024,
Volume and Issue:
unknown, P. 112220 - 112220
Published: Nov. 1, 2024
Language: Английский
Comparative study of chemometrics-enhanced spectrophotometric and liquid chromatographic techniques for the concurrent measurement of the effective pantoprazole and vincamine renal impairment combination therapy: Greenness, blueness, and whiteness appraisal
Sustainable Chemistry and Pharmacy,
Journal Year:
2024,
Volume and Issue:
42, P. 101803 - 101803
Published: Oct. 28, 2024
Language: Английский