Synthesis and use of new magnetic adsorbent for sensitive, practical and simultaneous analysis Ibuprofen and Ketoprofen molecules in urine samples DOI

Şule Temiz,

Songül Ulusoy,

Halil İbrahim Ulusoy

et al.

Journal of Chromatography B, Journal Year: 2024, Volume and Issue: 1251, P. 124404 - 124404

Published: Dec. 10, 2024

Language: Английский

Microextraction-Based Techniques for the Determination of Beta-Blockers in Biological Fluids: A Review DOI Creative Commons

Styliani Nisyriou,

Constantinos K. Zacharis

Separations, Journal Year: 2025, Volume and Issue: 12(1), P. 14 - 14

Published: Jan. 12, 2025

Sample preparation is a critical step in the analytical process. Apart from traditional sample cleanup approaches, microextraction-based techniques have attracted much attention recent years and especially researchers working analysis of beta-blockers. Developing sensitive selective methods essential for detecting these compounds complex matrices. The present review aims to provide an overview determination beta-blockers biological samples, covering time frame last decade. Detailed information on materials/coatings instrumental configurations are provided.

Language: Английский

Citations

1
Green Chemistry Approaches in the Analytical Validation of Fosravuconazole Using UV Spectrophotometry and RP-HPLC DOI Creative Commons

Jeswin Maliyakal,

Mital Patel

Green Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 100215 - 100215

Published: Jan. 1, 2025

Language: Английский

Citations

1
Development of a direct dilution method for the simultaneous determination of 40 metal and non-metallic elements in urine and blood samples within 6 minutes using ICP-MS: Application of comprehensive biomonitoring of elementomic phenotypes of individuals in paired biological samples. DOI

Yi Huang,

Jing Tian, Linying Wu

et al.

Talanta, Journal Year: 2025, Volume and Issue: 290, P. 127765 - 127765

Published: Feb. 18, 2025

Language: Английский

Citations

0
Development of a Catalyst‐Free Ultrasound‐Assisted Derivatization Method for Detection of Valproic Acid in Epilepsy Patient's Serum Using High‐Performance Liquid Chromatography: A Comparison With Chemiluminescence Immunoassay DOI

Javad Jaberi,

Nikoo Alavi,

Milad Moayednia

et al.

Journal of Separation Science, Journal Year: 2025, Volume and Issue: 48(2)

Published: Feb. 1, 2025

ABSTRACT The aim of this research was the therapeutic drug monitoring for valproic acid in epilepsy patient's serum samples by common, sensitive, and accessible HPLC‐UV method. Because absence a suitable chromophore structure, facile, selective, cost‐effective pre‐column derivatization designed. This catalyst‐free ultrasound‐assisted assay can accomplish very quickly only 5.0 min at mild temperature 60°C. 2,4ʹ‐Dibromoacetophenone nonanoic used as derivatizing agent internal standard, respectively. effect sample pH, buffer concentration, ultrasound exposure time, reaction temperature, amount were optimized. proposed method exhibited good linear range 5.0–300.0 µg/mL with acceptable correlation coefficients 0.9981. limit detection low 0.4 µg/mL. Also, quantification reported 1.3 Interday intraday relative standard deviations ( n = 10) 1.1% 0.3%–7.0%, In addition, recovery ranged from 100.3% to 107.7%. measurement performed presence several non‐epilepsy drugs developed protocol. confirmed specific accurate determination serum. A comparative evaluation employed against precise chemiluminescence immunoassay approach. coefficient between two methods 0.9992, which demonstrated results statistically same.

Language: Английский

Citations

0
Recent Advances in Bioanalysis of Cephalosporins Toward Green Sample Preparation DOI Creative Commons

Maria Kousi,

Natasa P. Kalogiouri, Victoria Samanidou

et al.

Journal of Separation Science, Journal Year: 2025, Volume and Issue: 48(2)

Published: Feb. 1, 2025

ABSTRACT This review highlights recent advances in the bioanalysis of cephalosporins using liquid chromatographic methods, focusing on green sample preparation (GSP) techniques. Cephalosporins, a class β‐lactam antibiotics, are critical combating bacterial infections but present challenges related to drug resistance and toxicity. article evaluates various including solid‐phase extraction, microextraction, protein precipitation, which have been employed extraction quantification from biological matrices. Special attention is given optimization parameters, such as pH, solvents, purification techniques maximize analytes’ recovery sensitivity. Emerging trends GSP, use molecularly imprinted polymers miniaturized processing devices, also discussed. The underscores growing importance integrating environmentally friendly approaches cephalosporin bioanalysis, paving way for future innovations bioanalytical research.

Language: Английский

Citations

0
Development and validation of an HPLC analytical method for the simultaneous estimation of vitamin B12 and folic acid DOI Creative Commons
Ruchi Singh, Harvinder Popli, Indranil Suryawanshi

et al.

Essential Chem, Journal Year: 2025, Volume and Issue: 2(1), P. 1 - 9

Published: Feb. 27, 2025

Language: Английский

Citations

0
A novel fluorescent sensor based on the reuse of a biological matrix for the visual detection of bilirubin DOI
Ying Yan,

Chunling Mao,

Haoran Xu

et al.

Journal of Molecular Structure, Journal Year: 2025, Volume and Issue: unknown, P. 142121 - 142121

Published: March 1, 2025

Language: Английский

Citations

0
Purification of testosterone and its metabolites in urine using two-dimensional high-performance liquid chromatography for 13C/12C ratios analysis by gas chromatography/combustion/isotope ratio mass spectrometry DOI
Mengmeng Yan,

Tianshuo Zhu,

Chao Wen

et al.

Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2025, Volume and Issue: 263, P. 116921 - 116921

Published: April 24, 2025

Language: Английский

Citations

0
Development and validation of a high-performance liquid chromatography method with fluorescence detection for the quantification of the resistance protein P-gp in cancer cells DOI Creative Commons

Elodie Gay,

Maxime Dubois,

Manon Roux

et al.

Journal of Chromatography B, Journal Year: 2025, Volume and Issue: 1253, P. 124475 - 124475

Published: Jan. 22, 2025

A method using high-performance liquid chromatography coupled with fluorescence detection (HPLC-FLD) was developed and validated to quantify the innovative tool LightSpot®-FL-1, a selective permeability-glycoprotein (P-gp)-targeted fluorescent conjugate used measure P-gp expression in cell samples. Quantifying is major challenge oncology as its overexpression many cancer cells results Multidrug Resistance (MDR) associated chemotherapy failure. To develop reported herein, both sample preparation analysis parameters were investigated. Optimal chromatographic conditions achieved 5 µL injections at 1 mL/min flow rate on reversed-phase Zorbax® Eclipse Plus 3.5 µm C18 column (150 × 4.6 mm) isocratic acetonitrile/water (85/15, by volume) elution. Detection performed 505 nm excitation 510 emission wavelengths. Validation studies designed according International Council for Harmonization (ICH) guidelines bioanalytical validation. The limit of quantification (LOQ) (LOD) determined be 0.5 0.2 nmol/L, respectively. linearity range demonstrated between 10 500 trueness precision validated. Good stability shown three relevant analytical conditions. greenness also AGREE, AGREEprep MoGAPI tools. Finally, rapid, precise sensitive successfully applied determine difference lines: DU4475, CCRF-CEM KG-1a.

Language: Английский

Citations

0
Live bacteria detection with high specificity by utilizing Eu3+@MIL-53 (Al) and bacteriophages-based fluorescence biosensor DOI

Qiaoli Yang,

Xiaohan Yang, Ke Guo

et al.

Talanta, Journal Year: 2025, Volume and Issue: unknown, P. 128060 - 128060

Published: April 1, 2025

Language: Английский

Citations

0