Mixed-Mode Chromatography: Studies on Hybrid Retention Mechanisms of Some Antihypertensive Drugs DOI Creative Commons

Irinel Adriana Badea,

Andrei Mihăilă,

Dana Elena Popa

et al.

Separations, Journal Year: 2025, Volume and Issue: 12(6), P. 136 - 136

Published: May 22, 2025

The antihypertensive drugs indapamide, atenolol, metoprolol, propranolol and bisoprolol were considered in this research. Because they have structures that are affected by pH, developing a chromatographic method was challenging. Based on the speciation diagram of these compounds versus pH scale, mixed-mode stationary phase (hydrophobic phase, C18 strong cation exchanger (SCX)) our first choice. Design Experiments (DoE) used to estimate how various factors such as mobile composition flow rate influenced performance. As result, separation achieved 24 min using an aqueous phosphate buffer (pH 7.20) 20 mM triethylamine, with methanol being organic modifier (30%). Their retention mechanism investigated. new validated term linearity, limits detection quantification, precision, accuracy, robustness. applied river water samples, good results obtained.

Language: Английский

The Effectiveness of Liquid-Phase Microextraction of Beta-Blockers from Aqueous Matrices for Their Analysis by Chromatographic Techniques DOI Creative Commons
Mihail Simion Beldean‐Galea, Mihaela-Cătălina Herghelegiu, Vlad-Alexandru Pănescu

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(5), P. 1016 - 1016

Published: Feb. 22, 2025

Beta-blockers are pharmaceuticals used to treat cardiovascular diseases such as hypertension, angina pectoris, and arrhythmia. Due high consumption, they continuously released into the environment, being detected in many aqueous matrices. The aim of this research is test effectiveness two green liquid-phase microextraction procedures, dispersive liquid-liquid (DLLME) solidification floating organic droplet (SFOME) for selective extraction eight beta-blockers (atenolol, nadolol, pindolol, acebutolol, metoprolol, bisoprolol, propranolol, betaxolol) from matrices their analysis by gas chromatography (GC) or liquid (LC). influence parameters, type volume disperser solvents, ionic strength were studied. developed procedures provide a good enrichment factor six compounds (61.22-243.97), recovery (53.04-92.1%), sample cleaning both procedures. Good limits detection (0.13 0.69 µg/mL GC 0.07 0.15 HPLC) quantification (0.39 2.10 0.20 0.45 LC) obtained. successfully applied selected wastewater samples, proving applicability real samples.

Language: Английский

Citations

0
Mixed-Mode Chromatography: Studies on Hybrid Retention Mechanisms of Some Antihypertensive Drugs DOI Creative Commons

Irinel Adriana Badea,

Andrei Mihăilă,

Dana Elena Popa

et al.

Separations, Journal Year: 2025, Volume and Issue: 12(6), P. 136 - 136

Published: May 22, 2025

The antihypertensive drugs indapamide, atenolol, metoprolol, propranolol and bisoprolol were considered in this research. Because they have structures that are affected by pH, developing a chromatographic method was challenging. Based on the speciation diagram of these compounds versus pH scale, mixed-mode stationary phase (hydrophobic phase, C18 strong cation exchanger (SCX)) our first choice. Design Experiments (DoE) used to estimate how various factors such as mobile composition flow rate influenced performance. As result, separation achieved 24 min using an aqueous phosphate buffer (pH 7.20) 20 mM triethylamine, with methanol being organic modifier (30%). Their retention mechanism investigated. new validated term linearity, limits detection quantification, precision, accuracy, robustness. applied river water samples, good results obtained.

Language: Английский

Citations

0