Metabolic characterization of alkane monooxygenases and the growth phenotypes of Pseudomonas aeruginosa ATCC 33988 on hydrocarbons DOI Creative Commons
Thusitha S. Gunasekera,

Loryn L. Bowen,

Jhoanna C. Alger

et al.

Journal of Bacteriology, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

ABSTRACT There is a demand and widespread interest in evaluating microbial community structures metabolic processes hydrocarbon environments. The current work aims to detect subgroups (phenotypic subsets) their processes, such as substrate specificity expression of niche-associated genes. In this study, we were able discriminate different cell types real time from complex sample matrix allow the detection live, dead, injured populations jet fuels. We found that alkB1 alkB2 genes induced growth-dependent manner induction started before . This indicates an early response Pseudomonas aeruginosa cells’ exposure alkanes, cells activate gene induction. Deletion completely inhibited P. ATCC 33988 growth fuel, suggesting two alkane monooxygenases are responsible for degradation alkanes fuel. Interestingly, AlkB2 has broader ( n -C8 -n -C16) range compared AlkB1 -C12- -C16). data indicate utilization pathways can coexist 33988, they differentially expressed -C6 -n- C16 study provided additional information on heterogeneity phenotypic diversity within same species after hydrocarbons. advances our understanding provides new insight into metabolism IMPORTANCE Alkane allows natural breakdown hydrocarbons crude oil, which significantly contribute environmental remediation. process microbes not well understood. originally isolated fuel tank, degrades effectively outcompetes type strain PAO1. differential monooxygenase relative importance these degradation. subsets genotype, influenced by stress. Overall, research conducted contributes knowledge about structure

Language: Английский

Inhibitory Effects of Nisin and Gallium (III) Nitrate Hydrate on Planktonic and Adhered Cells and Implications for the Viable but Non-Culturable State DOI Creative Commons
Valeria Poscente, Luciana Di Gregorio, Roberta Bernini

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 276 - 276

Published: Jan. 26, 2025

Effective antimicrobial and biofilm control strategies require an understanding of the differential effects agents on viability culturability microbial cells. A viable but non-culturable (VBNC) state, a survival strategy non-spore-forming bacteria in response to adverse conditions, poses significant challenge for public health food safety. In present study, we investigated antibiofilm nisin gallium (III) nitrate hydrate against Gram-positive strain Lactiplantibacillus plantarum subsp. DSM 20174 Gram-negative Pseudomonas fluorescens ATCC 13525, respectively. Both strains were chosen as model systems their relevance clinical settings. Culture-based methods flow cytometry (FCM) used evaluate both planktonic sessile cells, providing insights into physiological treatment-induced stress at different concentrations (100, 250, 350, 500 ppm). The findings highlight strain-specific action L. promising Ga P. fluorescens. This study underscores potential FCM powerful tool high-throughput analyses efficacy, valuable developing targeted safety applications.

Language: Английский

Citations

1
Identification of Pseudomonas protegens and Bacillus subtilis Antimicrobials for Mitigation of Fuel Biocontamination DOI Creative Commons
Amanda Barry, Alan Reed,

Osman Radwan

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(2), P. 227 - 227

Published: Feb. 4, 2025

Hydrocarbon fuel biofouling and biocorrosion require expensive cleanup of aviation infrastructures unless appropriate sustainment measures are applied. The identification novel biological control agents offers promising alternatives to the current chemical biocides used in sustainment. In this study, 496 microbial isolates from our in-house repository were screened identify new endogenously produced antimicrobial compounds. Using agar plug screening, liquid culture growth testing, Jet A assays, two fuel-isolate strains Pseudomonas protegens #133, Bacillus subtilis #232 demonstrated biocontrol activity against bacteria, yeast, filamentous fungi. Liquid chromatography-quadrupole time flight tandem mass spectrometry (LC-QTOF-MS/MS) filtrate identified several common lipopeptide antimicrobials including gageostatin C, gageopeptin B, miscellaneous macrolactins. contrast, LC-QTOF-MS/MS siderophore pyochelin as one predominant compounds #133 with previously effect. consortium testing flow-cytometry live/dead cell mechanism determination action Gram-positive bacteria. study concludes that secreted by have bactericidal effects Gordonia sp. cause death through bacterial lysis membrane damage potential applications biocidal treatment hydrocarbon-based fuels.

Language: Английский

Citations

1
Osmotic and Cold Stress-Induced Viable but Non-Culturable State in Lacticaseibacillus paracasei Zhang: A Transcriptome Analysis DOI
Qiuhua Bao, Bao Yuan, Xuebo Ma

et al.

International Dairy Journal, Journal Year: 2025, Volume and Issue: 166, P. 106228 - 106228

Published: Feb. 28, 2025

Language: Английский

Citations

0
Metabolic characterization of alkane monooxygenases and the growth phenotypes of Pseudomonas aeruginosa ATCC 33988 on hydrocarbons DOI Creative Commons
Thusitha S. Gunasekera,

Loryn L. Bowen,

Jhoanna C. Alger

et al.

Journal of Bacteriology, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

ABSTRACT There is a demand and widespread interest in evaluating microbial community structures metabolic processes hydrocarbon environments. The current work aims to detect subgroups (phenotypic subsets) their processes, such as substrate specificity expression of niche-associated genes. In this study, we were able discriminate different cell types real time from complex sample matrix allow the detection live, dead, injured populations jet fuels. We found that alkB1 alkB2 genes induced growth-dependent manner induction started before . This indicates an early response Pseudomonas aeruginosa cells’ exposure alkanes, cells activate gene induction. Deletion completely inhibited P. ATCC 33988 growth fuel, suggesting two alkane monooxygenases are responsible for degradation alkanes fuel. Interestingly, AlkB2 has broader ( n -C8 -n -C16) range compared AlkB1 -C12- -C16). data indicate utilization pathways can coexist 33988, they differentially expressed -C6 -n- C16 study provided additional information on heterogeneity phenotypic diversity within same species after hydrocarbons. advances our understanding provides new insight into metabolism IMPORTANCE Alkane allows natural breakdown hydrocarbons crude oil, which significantly contribute environmental remediation. process microbes not well understood. originally isolated fuel tank, degrades effectively outcompetes type strain PAO1. differential monooxygenase relative importance these degradation. subsets genotype, influenced by stress. Overall, research conducted contributes knowledge about structure

Language: Английский

Citations

0