Size Effect in the Compression of 3D Polymerized Micro-Structures DOI
Jiayu Li, Angelo Accardo,

Shutian Liu

et al.

Journal of Applied Mechanics, Journal Year: 2023, Volume and Issue: 91(1)

Published: July 25, 2023

Abstract Micro/nanoscale additive manufacturing provides a powerful tool for advanced materials and structures with complex precise features. For instance, the feature resolution of two-photon polymerization (2PP) can reach 200 nm. At this scale, properties change, influence size effect cannot be ignored. Therefore, it is necessary to assess changes in material mechanical considering effects. In work, several micrometric polymeric specimens are printed via 2PP, their assessed using compression tests. Detailed printing testing procedures effects parameter settings provided. The experimental results show that microstructures’ have direct on Young’s modulus. particular, large surface-volume ratio higher other words, smaller structure size, stiffness. reported findings play significant role development fabrication strategies microstructures where high stiffness accuracy fundamental.

Language: Английский

X-photon 3D lithography by fs-oscillators: wavelength-independent and photoinitiator-free DOI
Dimitra Ladika,

Antanas Butkus,

Vasileia Melissinaki

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 5(4), P. 567 - 567

Published: Jan. 1, 2024

Language: Английский

Citations

7
In Vitro Models for Investigating Intestinal Host–Pathogen Interactions DOI Creative Commons
Reece McCoy, Sophie Oldroyd,

Woojin Yang

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 11(8)

Published: Dec. 28, 2023

Infectious diseases are increasingly recognized as a major threat worldwide due to the rise of antimicrobial resistance and emergence novel pathogens. In vitro models that can adequately mimic in vivo gastrointestinal physiology high demand elucidate mechanisms behind pathogen infectivity, aid design effective preventive therapeutic interventions. There exists trade-off between simple throughput those more complex physiologically relevant. The complexity model used shall be guided by biological question addressed. This review provides an overview structure function intestine developed emulate this. Conventional discussed addition emerging which employ engineering principles equip them with necessary advanced monitoring capabilities for intestinal host-pathogen interrogation. Limitations current future perspectives on field presented.

Language: Английский

Citations

12
Multiphoton and Harmonic Imaging of Microarchitected Materials DOI Creative Commons
Brian W. Blankenship, Daisong Pan,

Eudokia Kyriakou

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: 17(2), P. 3887 - 3896

Published: Jan. 3, 2025

Microadditive manufacturing has revolutionized the production of complex, nano- to microscale components across various fields. This work investigates two-photon (2P) and three-photon (3P) fluorescence imaging, as well third-harmonic generation (THG) microscopy, examine periodic microarchitected lattice structures fabricated using multiphoton lithography (MPL). By immersing in refractive index matching fluids, we demonstrate high-fidelity 3D reconstructions both fluorescent 2P 3P microscopy low-fluorescence THG microscopy. These results show that (MPF) imaging offers reduced signal decay with respect depth compared single-photon techniques examined structures. We further ability nondestructively identify intentional internal modifications structure are not immediately visible scanning electron microscope (SEM) images compression-induced fractures, highlighting potential these for quality control defect detection microadditively manufactured components.

Language: Английский

Citations

0
Smart Polymeric 3D Microscaffolds Hosting Spheroids for Neuronal Research via Quantum Metrology DOI Creative Commons
Beatriz N. L. Costa, Ana Marote, Catarina Barbosa

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Toward the aim of reducing animal testing, innovative in vitro models are required. Here, this study proposes a novel smart polymeric microscaffold to establish an advanced 3D model dopaminergic neurons. These scaffolds fabricated with Ormocomp via Two-Photon Polymerization. The further enhanced by functionalization fluorescent nanodiamonds (FNDs), which can serve as quantum nanosensors for both magnetic and temperature sensing. material biocompatibility is tested using two different cell lines, SH-SY5Y A431, viability over 98%. A total 69% FNDs grafted on structure compared those that remained glass surface. Cells several microenvironments, final assembly required metrology experiments achieved 91% biocompatibility. Subsequently, embryoid bodies containing neurons, type affected Parkinson's disease (PD), integrated FND-functionalized scaffolds. This successfully established, demonstrated strong interactions between neurons scaffold, directional growth neurites along scaffold. Ultimately, have developed platform enables readout signaling holds great potential future PD research.

Language: Английский

Citations

0
Light-based 3D printing and post-treatments of moulds for PDMS soft lithography DOI Creative Commons
Bastien Venzac

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The fabrication of moulds by 3D printing for PDMS replication and their post-treatments to avoid curing inhibition are reviewed.

Language: Английский

Citations

0
Toward 3D printed microfluidic artificial lungs for respiratory support DOI Creative Commons
Elyse M. A. Fleck,

Charlise Keck,

Karolina Ryszka

et al.

Lab on a Chip, Journal Year: 2024, Volume and Issue: 24(4), P. 955 - 965

Published: Jan. 1, 2024

Development and testing of a small-scale 3D printed microfluidic artificial lung (μAL) fabricated via UV light based vat photopolymerization custom polydimethylsiloxane photopolymer resin.

Language: Английский

Citations

3
Deciphering the Influence of Effective Shear Modulus on Neuronal Network Directionality and Growth Cones’ Morphology via Laser‐Assisted 3D‐Printed Nanostructured Arrays DOI Creative Commons
George Flamourakis,

Qiangrui Dong,

Dimitri Kromm

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 21, 2024

Abstract In the present study, influence of topographic and mechanical cues on neuronal growth cones (NGCs) network directionality in 3D‐engineered cell culture models is explored. Two‐photon polymerization (2PP) employed to fabricate nanopillar arrays featuring tunable effective shear modulus. Large variations properties are obtained by altering aspect ratio nanostructures. The seeded with different lines, including neural progenitor cells (NPCs) derived from human induced pluripotent stem (iPSCs), I 3 Neurons, primary hippocampal neurons. All types exhibit preferential orientations according topology, as shown neurites creating a high number oriented orthogonal networks. Furthermore, differentiation maturation NPCs affected nanopillars, expression mature marker Synapsin I. Lastly, NGCs influenced modulus terms spreading area, stochastic optical reconstruction microscopy (STORM) assess cytoskeleton organization at nanometric resolution. developed approach, involving laser‐assisted 3D microfabrication, neuro‐mechanobiology, super‐resolution microscopy, paves way for prospective comparative studies evolution networks healthy diseased (e.g., neurodegenerative) conditions.

Language: Английский

Citations

2
Surface Tension-Based Open Microfluidic Platform Using Micropillars to Recreate the 3D Lung Cellular Microenvironment DOI
Tri Tho Yves Nguyen,

Jungseub Lee,

Sunbeen Choi

et al.

BioChip Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 5, 2024

Language: Английский

Citations

2
Characterization of Photocurable IP-PDMS for Soft Micro Systems Fabricated by Two-Photon Polymerization 3D Printing DOI Open Access
Rishikesh Srinivasaraghavan Govindarajan, Stanislav Sikulskyi, Zefu Ren

et al.

Polymers, Journal Year: 2023, Volume and Issue: 15(22), P. 4377 - 4377

Published: Nov. 10, 2023

Recent developments in micro-scale additive manufacturing (AM) have opened new possibilities state-of-the-art areas, including microelectromechanical systems (MEMS) with intrinsically soft and compliant components. While fabrication materials further complicates AM, a photocurable polydimethylsiloxane (PDMS) resin, IP-PDMS, has recently entered the market of two-photon polymerization (2PP) AM. To facilitate development microdevices components through application 2PP technique IP-PDMS material, this research paper presents comprehensive material characterization IP-PDMS. The significance study lies scarcity existing on thorough investigation its properties, many which are reported here for first time. Particularly, uncured work evaluates surface tension 26.7 ± 4.2 mN/m, contact angle glass 11.5 0.6°, spin-coating behavior, transmittance more than 90% above 440 nm wavelength, FTIR all properties For cured novel characterizations include small mechanical creep, velocity-dependent friction coefficient glass, typical dielectric permittivity value 2.63 0.02, high dielectric/breakdown strength 3D-printed elastomers up to 73.3 13.3 V/µm values spin coated elastomer 85.7 12.4 V/µm, while measured water 103.7 0.5°, Young's modulus 5.96 0.2 MPa, viscoelastic DMA compared previously values. Friction, permittivity, water, some breakdown measurements were performed spin-coated samples. Based characterization, shows itself be promising MEMS, microfluidics, storage devices, smart technologies.

Language: Английский

Citations

5
X-photon 3D lithography by fs-oscillators: wavelength-independent and photoinitiator-free DOI Creative Commons
Dimitra Ladika,

Antanas Butkus,

Vasileia Melissinaki

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 6, 2023

Abstract Laser direct writing employing multi-photon 3D polymerisation is a scientific and industrial tool used in various fields such as micro-optics, medicine, metamaterials, programmable materials, etc., due to the fusion of high-throughput fine features down hundreds nm. Some limitations technology applicability emerge from photo-resin properties, however any material modifications can strongly affect its printability, photoexcitation conditions alter well. Here we present wavelength-independent using low peak power laser oscillators. High pulse repetition rate fast was employed for advancing additive manufacturing out SZ2080 TM photo-resist without photo-initiator. Wavelengths 517 nm, 780 1035 nm are shown be suitable producing 300 polymerized even at high – up 100 mm/s speeds. Variation organic-inorganic ratio hybrid results shift decrease dynamic fabrication window, yet not prohibiting photo-structuring. Controlled energy deposition per focal volume achieved localized heating enabling efficient printing. Such spatio-selective photo-chemical cross-linking widens optical capacity non-photo-sensitive materials.

Language: Английский

Citations

5