Cell Reports,
Journal Year:
2019,
Volume and Issue:
26(5), P. 1174 - 1188.e5
Published: Jan. 1, 2019
Neuronal
activity-inducible
gene
transcription
correlates
with
rapid
and
transient
increases
in
histone
acetylation
at
promoters
enhancers
of
activity-regulated
genes.
Exactly
how
modulates
these
genes
has
remained
unknown.
We
used
single-cell
situ
transcriptional
analysis
to
show
that
Fos
Npas4
are
transcribed
stochastic
bursts
mouse
neurons
membrane
depolarization
mRNA
expression
by
increasing
burst
frequency.
then
expressed
dCas9-p300
or
dCas9-HDAC8
fusion
proteins
mimic
block
activity-induced
locally
enhancers.
Adding
increased
prolonging
duration
resulted
higher
protein
levels
an
elevation
resting
potential.
Inhibiting
reduced
reducing
frequency
impaired
experience-dependent
induction
the
hippocampus
vivo.
Thus,
tunes
dynamics
experience-regulated
affect
selective
changes
neuronal
cellular
function.
Molecular Cell,
Journal Year:
2023,
Volume and Issue:
83(3), P. 373 - 392
Published: Jan. 23, 2023
Uncovering
the
cis-regulatory
code
that
governs
when
and
how
much
each
gene
is
transcribed
in
a
given
genome
cellular
state
remains
central
goal
of
biology.
Here,
we
discuss
major
layers
regulation
influence
transcriptional
outputs
are
encoded
by
DNA
sequence
context.
We
first
transcription
factors
bind
specific
sequences
dosage-dependent
cooperative
manner
then
proceed
to
cofactors
facilitate
factor
function
mediate
activity
modular
elements
such
as
enhancers,
silencers,
promoters.
consider
complex
poorly
understood
interplay
these
diverse
within
regulatory
landscapes
its
relationships
with
chromatin
states
nuclear
organization.
propose
mechanistically
informed,
quantitative
model
integrates
multiple
will
be
key
ultimately
cracking
code.
Clinical Epigenetics,
Journal Year:
2021,
Volume and Issue:
13(1)
Published: July 8, 2021
Abstract
Transcriptionally
active
chromatin
is
marked
by
tri-methylation
of
histone
H3
at
lysine
4
(H3K4me3)
located
after
first
exons
and
around
transcription
start
sites.
This
epigenetic
mark
typically
restricted
to
narrow
regions
the
5`end
gene
body,
though
a
small
subset
genes
have
broad
H3K4me3
domain
which
extensively
covers
coding
region.
Although
most
studies
focus
on
mark,
associated
with
plethora
modifications
(e.g.,
acetylated
K27)
therein
termed
domain.
Genes
are
involved
in
cell
identity
essential
functions
clinical
potential
as
biomarkers
for
patient
stratification.
Reducing
expression
may
increase
metastatic
cancer
cells.
Enhancers
super-enhancers
interact
forming
hub
interactions
involving
nucleosome-depleted
regions.
Together,
regulatory
elements
coalesce
factors,
modifying/remodeling
enzymes,
coactivators,
Mediator
and/or
Integrator
complex
into
factory
be
analogous
liquid–liquid
phase-separated
condensate.
The
has
dynamic
structure
supports
frequent
bursts.
In
this
review,
we
present
current
knowledge
domains.
Cell Reports,
Journal Year:
2019,
Volume and Issue:
26(5), P. 1174 - 1188.e5
Published: Jan. 1, 2019
Neuronal
activity-inducible
gene
transcription
correlates
with
rapid
and
transient
increases
in
histone
acetylation
at
promoters
enhancers
of
activity-regulated
genes.
Exactly
how
modulates
these
genes
has
remained
unknown.
We
used
single-cell
situ
transcriptional
analysis
to
show
that
Fos
Npas4
are
transcribed
stochastic
bursts
mouse
neurons
membrane
depolarization
mRNA
expression
by
increasing
burst
frequency.
then
expressed
dCas9-p300
or
dCas9-HDAC8
fusion
proteins
mimic
block
activity-induced
locally
enhancers.
Adding
increased
prolonging
duration
resulted
higher
protein
levels
an
elevation
resting
potential.
Inhibiting
reduced
reducing
frequency
impaired
experience-dependent
induction
the
hippocampus
vivo.
Thus,
tunes
dynamics
experience-regulated
affect
selective
changes
neuronal
cellular
function.
