The epigenetic basis of cellular heterogeneity

Nature Reviews Genetics, Journal Year: 2020, Volume and Issue: 22(4), P. 235 - 250

Published: Nov. 26, 2020

Language: Английский

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Spatial profiling technologies illuminate the tumor microenvironment

Cancer Cell, Journal Year: 2023, Volume and Issue: 41(3), P. 404 - 420

Published: Feb. 16, 2023

Language: Английский

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Chromosome Conformation Capture and Beyond: Toward an Integrative View of Chromosome Structure and Function

Molecular Cell, Journal Year: 2020, Volume and Issue: 77(4), P. 688 - 708

Published: Jan. 27, 2020

Language: Английский

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Transcription in Living Cells: Molecular Mechanisms of Bursting

Annual Review of Biochemistry, Journal Year: 2020, Volume and Issue: 89(1), P. 189 - 212

Published: March 25, 2020

Transcription in several organisms from certain bacteria to humans has been observed be stochastic nature: toggling between active and inactive states. Periods of nascent RNA synthesis known as bursts represent individual gene activation events which multiple polymerases are initiated. Therefore, bursting is the single locus illustration both repression. Although transcriptional was originally decades ago, only recently have technological advances enabled field begin elucidating regulation at single-locus level. In this review, we focus on how biochemical, genomic, single-cell data describe regulatory steps bursts.

Language: Английский

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Spatially resolved single-cell genomics and transcriptomics by imaging

Nature Methods, Journal Year: 2021, Volume and Issue: 18(1), P. 18 - 22

Published: Jan. 1, 2021

Language: Английский

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In situ genome sequencing resolves DNA sequence and structure in intact biological samples

Science, Journal Year: 2021, Volume and Issue: 371(6532)

Published: Feb. 25, 2021

Understanding genome organization requires integration of DNA sequence and three-dimensional spatial context; however, existing genome-wide methods lack either base pair resolution or direct localization. Here, we describe in situ sequencing (IGS), a method for simultaneously imaging genomes within intact biological samples. We applied IGS to human fibroblasts early mouse embryos, spatially localizing thousands genomic loci individual nuclei. Using these data, characterized parent-specific changes structure across embryonic stages, revealed single-cell chromatin domains zygotes, uncovered epigenetic memory global chromosome positioning embryos. These results demonstrate how can directly connect length scales from single pairs whole organisms.

Language: Английский

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Stabilization of chromatin topology safeguards genome integrity

Nature, Journal Year: 2019, Volume and Issue: 574(7779), P. 571 - 574

Published: Oct. 23, 2019

Language: Английский

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LifeTime and improving European healthcare through cell-based interceptive medicine

Nature, Journal Year: 2020, Volume and Issue: 587(7834), P. 377 - 386

Published: Sept. 7, 2020

Language: Английский

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Cis-regulatory chromatin loops arise before TADs and gene activation, and are independent of cell fate during early Drosophila development

Nature Genetics, Journal Year: 2021, Volume and Issue: 53(4), P. 477 - 486

Published: April 1, 2021

Language: Английский

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The spatial organization of transcriptional control

Nature Reviews Genetics, Journal Year: 2022, Volume and Issue: 24(1), P. 53 - 68

Published: Sept. 14, 2022

Language: Английский

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