Long non‐coding RNAs as key regulators of neurodegenerative protein aggregation DOI Creative Commons

Qi Xu,

Dan Liu,

Ling‐Qiang Zhu

et al.

Alzheimer s & Dementia, Journal Year: 2025, Volume and Issue: 21(2)

Published: Feb. 1, 2025

Abstract The characteristic events in neurodegenerative diseases (NDDs) encompass protein misfolding, aggregation, accumulation, and their related cellular dysfunction, synaptic function loss. While distinct proteins are implicated the pathological processes of different NDDs, process misfolding aggregation remains notably similar across various conditions. Specifically, undergo into beta‐folded (β‐folded) conformation, resulting formation insoluble amyloid proteins. Despite advancements comprehending certain facets this intricate remain incompletely elucidated. In recent years, concept that long non‐coding RNAs (lncRNAs) contribute to has gained recognition. LncRNAs influence aggregates by facilitating overexpression through regulation gene transcription translation, inhibiting degradation via lysosomal autophagic pathways, targeting aberrant modifications phase transitions A better understanding relationship between lncRNAs is an important step dissecting underlying molecular mechanisms will discovery new therapeutic targets strategies. Highlights NDDs marked leading dysfunction loss function. being involved β‐folded conformations forming consistent role attention, as they regulate inhibit degradation, target modifications. Understanding link crucial for uncovering developing targets.

Language: Английский

N4-acetylcytidine modification of lncRNA CTC-490G23.2 promotes cancer metastasis through interacting with PTBP1 to increase CD44 alternative splicing DOI
Xiaomei Yu,

Shujun Li,

Ziting Yao

et al.

Oncogene, Journal Year: 2023, Volume and Issue: 42(14), P. 1101 - 1116

Published: Feb. 16, 2023

Language: Английский

Citations

43
Downregulation of LncRNA CCAT1 Enhances Chemosensitivity in Cisplatin‐Resistant Gastric Cancer Cells DOI Open Access
Qiong Wu, Chenglou Zhu, Tiantian Zhao

et al.

Drug Development Research, Journal Year: 2025, Volume and Issue: 86(1)

Published: Jan. 20, 2025

ABSTRACT Chemotherapy is an effective treatment for gastric cancer. However, many patients develop resistance to chemotherapeutic agents during clinical treatment. LncRNA CCAT1 has recently been shown influence cellular specific drugs, but its role in cancer remains underexplored. This study aims investigate the of cisplatin cells and potential underlying mechanisms. Gastric cell lines with acquired were established. The expression was assessed both cisplatin‐sensitive cisplatin‐resistant AGS lines. knocked down AGS/DDP cells, changes IC50 values measured using Cell Counting Kit‐8 (CCK‐8) assay. Apoptosis evaluated by flow cytometry. Additionally, Western blotting employed measure levels PI3K/AKT/mTOR signaling pathway proteins apoptosis‐related interference control groups. RT‐qPCR results indicated that significantly elevated compared non‐resistant cells. Similarly, CCK‐8 assay demonstrated knocking resistant increased their sensitivity Flow cytometry blot further confirmed silencing promoted apoptosis these higher sensitive counterparts, resulted reduced proteins. In conclusion, above studies induced

Language: Английский

Citations

2
RNA Sequencing Reveals the Long Non‐Coding RNA Signature in Psoriasis Keratinocytes and Identifies CYDAER as a Long Non‐Coding RNA Regulating Epidermal Differentiation DOI Creative Commons
Jan Cedric Freisenhausen, Longlong Luo, E. Kelemen

et al.

Experimental Dermatology, Journal Year: 2025, Volume and Issue: 34(2)

Published: Feb. 1, 2025

ABSTRACT Psoriasis is a common chronic inflammatory skin disease determined by genetic and environmental factors, resulting in the activation of IL‐23/IL‐17‐mediated immune response, epidermal hyperproliferation, keratinocyte activation. Long non‐coding RNAs (lncRNAs) are non‐protein‐coding transcripts > 500 nucleotides with diverse regulatory functions; their role dysfunction psoriasis poorly understood. To identify potential roles psoriasis, including lncRNAs, we performed RNA sequencing on keratinocytes from healthy skin. We identified 889 differentially expressed many which yet unknown functions. RP11‐295G20.2 was as lncRNA significantly induced keratinocytes, this verified qRT‐PCR single‐molecule situ hybridisation. Analysis subcellular fractions epidermis revealed cytoplasmic localisation line results single molecule report that has skin‐enriched expression, within it mainly suprabasal layers. Moreover, key cytokine IL‐17A shows dynamic regulation during differentiation upregulation early downregulation late stage. Knockdown promotes terminal differentiation. Based our findings, named C ytoplasmic D ifferentiation‐ A ssociated Epidermal R NA, CYDAER. In summary, study provides comprehensive characterisation landscape identifies CYDAER regulating Our data suggest overexpression may contribute to altered psoriatic epidermis.

Language: Английский

Citations

2
Exosome‐Based Theranostic for Gastrointestinal Cancer: Advances in Biomarker Discovery and Therapeutic Engineering DOI Creative Commons
Mohit J. Mehta,

Dongjun Shin,

Hyun Sung Park

et al.

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Abstract Exosomes have emerged as versatile biomolecules in gastrointestinal (GI) cancer management, leveraging their inherent cargo‐carrying capabilities to overcome the limitations of current diagnostic and therapeutic strategies. This review provides a comprehensive exploration exosome‐based technologies, highlighting dual roles biomarkers vehicles. On front, this investigates specific exosomal biomarkers—including miRNAs, lncRNAs, circRNAs, proteins—emphasizing tumor biology clinical utility, such accuracy, early detection potential, prognostic significance. Therapeutically, study examines sources, purification methodologies, engineering strategies that enhance efficacy exosomes modulating immune responses, overcoming drug resistance, suppressing metastasis. By providing overview field, serves guideline for researchers clinicians entering offering novel insights into potential theranostic systems delineating future directions existing challenges.

Language: Английский

Citations

2
Cancer Epigenetics: An Overview DOI Open Access
Félix Recillas‐Targa

Archives of Medical Research, Journal Year: 2022, Volume and Issue: 53(8), P. 732 - 740

Published: Nov. 18, 2022

Language: Английский

Citations

62
Emerging functions of mitochondria-encoded noncoding RNAs DOI Creative Commons

Bingbing Ren,

Min‐Xin Guan, Tianhua Zhou

et al.

Trends in Genetics, Journal Year: 2022, Volume and Issue: 39(2), P. 125 - 139

Published: Sept. 19, 2022

Mitochondria, organelles that harbor their own circular genomes, are critical for energy production and homeostasis maintenance in eukaryotic cells. Recent studies discovered hundreds of mitochondria-encoded noncoding RNAs (mt-ncRNAs), including novel subtypes (mecciRNAs) double-stranded (mt-dsRNAs). Here, we discuss the emerging field mt-ncRNAs by reviewing expression patterns, biogenesis, metabolism, regulatory roles, functional mechanisms. Many have roles cellular physiology, some associated with, or even act as, causal factors human diseases. We also highlight developments technologies methodologies further insights into future perspectives challenges studying these RNAs, as well potential biomedical applications.

Language: Английский

Citations

41
The NR_109/FUBP1/c-Myc axis regulates TAM polarization and remodels the tumor microenvironment to promote cancer development DOI Creative Commons
Cong Zhang, Sisi Wei, Suli Dai

et al.

Journal for ImmunoTherapy of Cancer, Journal Year: 2023, Volume and Issue: 11(5), P. e006230 - e006230

Published: May 1, 2023

Background Tumor-associated macrophages (TAMs) are a major component of the tumor microenvironment (TME) and exert an important role in progression. Due to heterogeneity plasticity TAMs, modulating polarization states TAMs is considered as potential therapeutic strategy for tumors. Long noncoding RNAs (lncRNAs) have been implicated various physiological pathological processes, yet underlying mechanism on how lncRNAs manipulate still unclear remains be further investigated. Methods Microarray analyses were employed characterize lncRNA profile involved THP-1-induced M0, M1 M2-like macrophage. Among those differentially expressed lncRNAs, NR_109 was studied, its function macrophage effects condition medium or mediated by proliferation, metastasis TME remodeling both vitro vivo. Moreover, we revealed interacted with far upstream element-binding protein 1 (FUBP1) regulate stability through hindering ubiquitination modification competitively binding JVT-1. Finally, examined sections patients probe correlation among expression related proteins, showing clinical significance NR_109. Results We found that highly macrophages. Knockdown impeded IL-4 induced significantly reduced activity support proliferation cells Mechanistically, competed JVT-1 bind FUBP1 at C-terminus domain, ubiquitin-mediated degradation FUBP1, activated c-Myc transcription thus promoted polarization. Meanwhile, factor, could promoter enhance Clinically, high CD163 + from tissues positively correlated poor stages gastric cancer breast cancer. Conclusions Our work first time exerted crucial regulating phenotype-remodeling via NR_109/FUBP1/c-Myc positive feedback loop. Thus, has great translational potentials diagnosis, prognosis immunotherapy

Language: Английский

Citations

31
Single-cell transcriptomic analysis uncovers diverse and dynamic senescent cell populations DOI Creative Commons
Noah Wechter, Martina Rossi, Carlos Anerillas

et al.

Aging, Journal Year: 2023, Volume and Issue: unknown

Published: April 19, 2023

Aging | doi:10.18632/aging.204666. Noah Wechter, Martina Rossi, Carlos Anerillas, Dimitrios Tsitsipatis, Yulan Piao, Jinshui Fan, Jennifer L. Martindale, Supriyo De, Krystyna Mazan-Mamczarz, Myriam Gorospe

Language: Английский

Citations

26
Photobiomodulation promotes spinal cord injury repair by inhibiting macrophage polarization through lncRNA TUG1-miR-1192/TLR3 axis DOI Creative Commons
Cheng Ju, Yangguang Ma,

Xiaoshuang Zuo

et al.

Cellular & Molecular Biology Letters, Journal Year: 2023, Volume and Issue: 28(1)

Published: Jan. 19, 2023

Secondary spinal cord injury (SCI) often causes the aggravation of inflammatory reaction and nerve injury, which affects recovery motor function. Bone-marrow-derived macrophages (BMDMs) were recruited to injured area after SCI, M1 polarization is key process for inducing response neuronal apoptosis. We previously showed that photobiomodulation (PBM) can inhibit phenotype BMDMs reduce inflammation, but underlying mechanisms are unclear. The purpose this study explore potential target mechanism PBM in treating SCI.Transcriptome sequencing bioinformatics analysis long noncoding RNA taurine upregulated gene 1 (lncRNA TUG1) was a PBM. expression specific lncRNA TUG1 detected by qPCR, immunofluorescence, flow cytometry, western blotting, fluorescence situ hybridization, luciferase assay. Basso mouse scale (BMS) gait used evaluate function mice.Results may be PBM, regulating BMDMs, response, axial growth DRG. Mechanistically, competed with TLR3 binding miR-1192 attenuated inhibitory effect on TLR3. This protected from degradation, enabling high TLR3, promoted activation downstream NF-κB signal release cytokines. In vivo, treatment could TUG1, cytokines survival SCI mice.Our clarified TUG1/miR-1192/TLR3 axis an important pathway macrophage provides theoretical support its clinical application patients SCI.

Language: Английский

Citations

23
The Long Non-Coding RNA ANRIL in Cancers DOI Open Access

Aymeric Sanchez,

Julien Lhuillier,

Guillaume Grosjean

et al.

Cancers, Journal Year: 2023, Volume and Issue: 15(16), P. 4160 - 4160

Published: Aug. 17, 2023

ANRIL (Antisense Noncoding RNA in the INK4 Locus), a long non-coding encoded human chromosome 9p21 region, is critical factor for regulating gene expression by interacting with multiple proteins and miRNAs. It has been found to play important roles various cellular processes, including cell cycle control proliferation. Dysregulation of associated several diseases like cancers cardiovascular diseases, instance. Understanding oncogenic role its potential as diagnostic prognostic biomarker cancer crucial. This review provides insights into regulatory mechanisms significance locus cancer.

Language: Английский

Citations

23