Advanced domestication: harnessing the precision of gene editing in crop breeding DOI
Wendy J. Lyzenga, Curtis Pozniak, Sateesh Kagale

et al.

Plant Biotechnology Journal, Journal Year: 2021, Volume and Issue: 19(4), P. 660 - 670

Published: March 4, 2021

Summary Human population growth has increased the demand for food crops, animal feed, biofuel and biomaterials, all while climate change is impacting environmental conditions. There an urgent need to develop crop varieties which tolerate adverse conditions requiring fewer inputs. Plant breeding critical global security and, it benefited from modern technologies, remains constrained by a lack of valuable genetic diversity, linkage drag, effective way combine multiple favourable alleles complex traits. CRISPR/Cas technology transformed genome editing across biological systems promises transform agriculture with its high precision, ease design, multiplexing ability low cost. We discuss integration CRISPR/Cas‐based gene into advance domestication refine inbred various applications environments. highlight use fix desirable allelic variants, generate novel alleles, break deleterious linkages, support pre‐breeding introgression loci elite lines.

Language: Английский

Applications of CRISPR–Cas in agriculture and plant biotechnology DOI
Haocheng Zhu, Chao Li, Caixia Gao

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2020, Volume and Issue: 21(11), P. 661 - 677

Published: Sept. 24, 2020

Language: Английский

Citations

652
Precise plant genome editing using base editors and prime editors DOI
Kutubuddin A. Molla, Simon Sretenovic, K. C. Bansal

et al.

Nature Plants, Journal Year: 2021, Volume and Issue: 7(9), P. 1166 - 1187

Published: Sept. 13, 2021

Language: Английский

Citations

256
CRISPR/Cas: A powerful tool for gene function study and crop improvement DOI Creative Commons
Dangquan Zhang, Zhiyong Zhang, Turgay Ünver

et al.

Journal of Advanced Research, Journal Year: 2020, Volume and Issue: 29, P. 207 - 221

Published: Oct. 21, 2020

It is a long-standing goal of scientists and breeders to precisely control gene for studying its function as well improving crop yield, quality, tolerance various environmental stresses. The discovery modification CRISPR/Cas system, nature-occurred editing tool, opens an era precision breeding.In this review, we first introduce the brief history followed mechanism application system on study improvement. Currently, genome has been becoming mature cutting-edge biotechnological tool improvement that already used in many different traits crops, including pathogen resistance, abiotic tolerance, plant development morphology even secondary metabolism fiber development. Finally, point out major issues associating with future research directions.Key Scientific Concepts Review: CRISPR/Cas9 robust powerful targeting individual DNA RNA sequence genome. can be target knockin, knockout replacement monitoring regulating expression at epigenome levels by binding specific sequence. Agrobacterium-mediated method still efficient delivering regents into targeted cells. However, other delivery methods, such virus-mediated method, have developed enhanced potentials CRISPR/Cas9-based PAM requirement offers CRISPR/Cas9-targted genetic loci also limits CRISPR/Cas9. Discovering new Cas proteins modifying current enzymes play important role editing. Developing better methods eliminating off-target effects, finding key/master genes controlling growth two directions

Language: Английский

Citations

253
PAM-less plant genome editing using a CRISPR–SpRY toolbox DOI

Qiurong Ren,

Simon Sretenovic, Shishi Liu

et al.

Nature Plants, Journal Year: 2021, Volume and Issue: 7(1), P. 25 - 33

Published: Jan. 4, 2021

Language: Английский

Citations

186
Development of Plant Prime-Editing Systems for Precise Genome Editing DOI Creative Commons

Rongfang Xu,

Juan Li, Xiaoshuang Liu

et al.

Plant Communications, Journal Year: 2020, Volume and Issue: 1(3), P. 100043 - 100043

Published: April 8, 2020

Prime-editing systems have the capability to perform efficient and precise genome editing in human cells. In this study, we first developed a plant prime editor 2 (pPE2) system test its activity by generating targeted mutation on an HPT-ATG reporter rice. Our results showed that pPE2 could induce programmable at different sites. transgenic T0 plants, pPE2-generated mutants occurred with 0%–31.3% frequency, suggesting efficiency of varied greatly genomic sites prime-editing guide RNAs diverse structures. To optimize efficiency, were introduced into following PE3 PE3b strategy However, tested pPE3 generated only comparable or even lower frequencies. Furthemore, surrogate incorporating enrich prime-edited The nucleotide was easily detected resistant calli transformed system, presumably due enhanced screening edited Taken together, our indicate provide versatile flexible rice genome.

Language: Английский

Citations

176
Natural Variation in Crops: Realized Understanding, Continuing Promise DOI Open Access
Yameng Liang, Haijun Liu, Jianbing Yan

et al.

Annual Review of Plant Biology, Journal Year: 2021, Volume and Issue: 72(1), P. 357 - 385

Published: Jan. 22, 2021

Crops feed the world's population and shape human civilization. The improvement of crop productivity has been ongoing for almost 10,000 years evolved from an experience-based to a knowledge-driven practice over past three decades. Natural alleles their reshuffling are long-standing genetic changes that affect how crops respond various environmental conditions agricultural practices. Decoding basis natural variation is central understanding evolution and, in turn, improving breeding. Here, we review current advances approaches used map causal variation, provide refined insights into genetics outline this knowledge promises drive development sustainable agriculture under dome emerging technologies.

Language: Английский

Citations

111
The integrated genomics of crop domestication and breeding DOI Creative Commons
Xuehui Huang, Sanwen Huang, Bin Han

et al.

Cell, Journal Year: 2022, Volume and Issue: 185(15), P. 2828 - 2839

Published: May 27, 2022

Language: Английский

Citations

99
Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants DOI Creative Commons
Manoj Kumar, Manas Ranjan Prusty, Manish K. Pandey

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: April 18, 2023

Abiotic stresses, including drought, salinity, cold, heat, and heavy metals, extensively reducing global agricultural production. Traditional breeding approaches transgenic technology have been widely used to mitigate the risks of these environmental stresses. The discovery engineered nucleases as genetic scissors carry out precise manipulation in crop stress-responsive genes associated molecular network has paved way for sustainable management abiotic stress conditions. In this context, clustered regularly interspaced short palindromic repeat-Cas (CRISPR/Cas)-based gene-editing tool revolutionized due its simplicity, accessibility, adaptability, flexibility, wide applicability. This system great potential build up varieties with enhanced tolerance against review, we summarize latest findings on understanding mechanism response plants application CRISPR/Cas-mediated towards a multitude stresses metals. We provide mechanistic insights CRISPR/Cas9-based genome editing technology. also discuss applications evolving techniques such prime base editing, mutant library production, transgene free multiplexing rapidly deliver modern cultivars adapted

Language: Английский

Citations

85
Targeted genome-modification tools and their advanced applications in crop breeding DOI
Boshu Li, Chao Sun, Jiayang Li

et al.

Nature Reviews Genetics, Journal Year: 2024, Volume and Issue: 25(9), P. 603 - 622

Published: April 24, 2024

Language: Английский

Citations

58
Advances in genomic tools for plant breeding: harnessing DNA molecular markers, genomic selection, and genome editing DOI Creative Commons
Rahul Kumar,

Sankar Prasad Das,

Burhan U. Choudhury

et al.

Biological Research, Journal Year: 2024, Volume and Issue: 57(1)

Published: Nov. 7, 2024

Abstract Conventional pre-genomics breeding methodologies have significantly improved crop yields since the mid-twentieth century. Genomics provides breeders with advanced tools for whole-genome study, enabling a direct genotype–phenotype analysis. This shift has led to precise and efficient development through genomics-based approaches, including molecular markers, genomic selection, genome editing. Molecular such as SNPs, are crucial identifying regions linked important traits, enhancing accuracy efficiency. Genomic resources viz. genetic reference genomes, sequence protein databases, transcriptomes, gene expression profiles, vital in plant aid identification of key understanding diversity, assist mapping, support marker-assisted selection speeding up programs. Advanced techniques like CRISPR/Cas9 allow modification, accelerating processes. Key Genome-Wide Association study (GWAS), Marker-Assisted Selection (MAS), (GS) enable trait prediction outcomes, improving yield, disease resistance, stress tolerance. These handy complex traits influenced by multiple genes environmental factors. paper explores new technologies editing showcasing their impact on developing varieties.

Language: Английский

Citations

18