Molecular Plant, Journal Year: 2022, Volume and Issue: 15(8), P. 1266 - 1267
Published: July 16, 2022
Language: Английский
Genome biology, Journal Year: 2024, Volume and Issue: 25(1)
Published: March 11, 2024
Abstract Background Bromo-adjacent homology-plant homeodomain domain containing protein 1 (BP1) is a reader of histone post-translational modifications in fungi. BP1 recognizes trimethylation lysine 27 H3 (H3K27me3), an epigenetic hallmark gene silencing. However, whether and how participates transcriptional repression remains poorly understood. Results We report that forms phase-separated liquid condensates to modulate its biological function Fusarium graminearum . Deletion assays reveal intrinsically disordered region 2 (IDR2) mediates liquid–liquid phase separation. The separation indispensable for interaction with suppressor Zeste 12, component polycomb repressive complex 2. Furthermore, IDR2 deletion abolishes BP1-H3K27me3 binding alleviates the secondary metabolism-related genes, especially deoxynivalenol mycotoxin biosynthesis genes. Conclusions maintains by forming condensates, expanding our understanding relationship between
Language: Английский
Citations
9
Cell Death and Disease, Journal Year: 2023, Volume and Issue: 14(11)
Published: Nov. 15, 2023
Abstract DNA double-strand breaks (DSBs) are the fatal type of damage mostly induced by exposure genome to ionizing radiation or genotoxic chemicals. DSBs mainly repaired homologous recombination (HR) and nonhomologous end joining (NHEJ). To repair DSBs, a large amount factors was observed be concentrated at in specific spatiotemporal manner form center. Recently, this center characterized as condensate derived from liquid-liquid phase separation (LLPS) key factors. LLPS has been found mechanism membraneless organelles formation plays roles variety biological processes. In review, recent advances mechanisms repair-related condensates summarized.
Language: Английский
Citations
20
Plant Communications, Journal Year: 2023, Volume and Issue: 5(1), P. 100663 - 100663
Published: July 26, 2023
Membraneless biomolecular condensates play important roles in both normal biological activities and responses to environmental stimuli living organisms. Liquid‒liquid phase separation (LLPS) is an organizational mechanism that has emerged recent years explain the formation of condensates. In past decade, advances LLPS research have contributed breakthroughs disease fields. By contrast, although plants progressed over 5 years, it been concentrated on model plant Arabidopsis, which limited relevance agricultural production. this review, we provide overview recently reported plants, with a particular focus photomorphogenesis, flowering, abiotic biotic stress responses. We propose many potential proteins also exist crops may affect crop growth, development, resistance. This possibility presents great challenge as well opportunity for rigorous scientific functions applications crops.
Language: Английский
Citations
16
New Crops, Journal Year: 2024, Volume and Issue: 1, P. 100026 - 100026
Published: May 16, 2024
In the rapidly evolving field of biology, phase separation has recently emerged as a revolutionary perspective, shedding new light on our comprehension cellular processes. This review provides comprehensive overview current knowledge regarding in plants and charts promising avenues for future exploration. We delve into fundamental principles plant separation, highlighting roles played by intrinsically disordered regions prion-like domains. Summarizing significant advancements, we explore involvement responses to environmental cues, well its growth developmental processes, plant-microbe interactions. Additionally, present streamlined workflow designed guide scientific community conducting studies plants. Lastly, delineate lingering questions propose potential applications agriculture.
Language: Английский
Citations
4
Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)
Published: March 22, 2025
The widespread application of ionizing radiation (IR) in medicine, while beneficial, also poses potential risks that necessitate effective countermeasures. Both 2-(3-aminopropylamino) ethanethiol (WR-1065) and curcumin are recognized as radioprotective agents; however, their clinical utility is hindered by notable shortcomings could be addressed through reactive oxygen species (ROS)-responsive amphiphilic nanomaterials. We introduced a newly synthesized poly (ethylene glycol) (PEG)-polycaprolactone (PCL) polymer integrated with diselenide bonds (HOOC-SeSe-Cur-PEG-SeSe-Cur-PCL, PEG-Cur-SeSe-PCL). resulting spherical nanoparticles (NPs), which self-assembled from this polymer, were uniform an average diameter 118 nm. As carrier for WR-1065, these NPs demonstrated loading capacity 30.9% efficacy 56.7%. Importantly, the degradation WR-1065 within was minimal gastric fluid, decreasing only approximately 20% over 6-hour period. innovative aspect design to destabilize ROS-rich environments, facilitating release curcumin. Indeed, survival rate mice increased 50% when orally administered prior exposure lethal dose whole-body irradiation (8 Gy). impact WR-1065-loaded evident small intestine irradiated mice, characterized amelioration radiation-induced epithelial damage, reduction DNA inhibition apoptotic pathway. Collectively, oral nanocarrier system holds promise candidate prophylaxis treatment acute intestinal injuries induced IR.
Language: Английский
Citations
0
Cell Proliferation, Journal Year: 2025, Volume and Issue: unknown
Published: April 11, 2025
ABSTRACT Membraneless organelles (MLOs) are a type of subcellular compartment structure discovered in eukaryotes recent years. They mainly formed through the liquid–liquid phase separation (LLPS) and aggregation macromolecular substances such as proteins or nucleic acids cells. When cells stimulated, they initiate series stress responses including gene transcription, RNA metabolism, translation, protein modification signal transduction to maintain homeostasis. The dysregulation these cellular processes is key event occurrence development cancer. This article provides an overview function membraneless organelles, well mechanisms separation, summarise latest research progress on tumours. It focuses role molecular mechanism LLPS tumours, with aim providing new theoretical references for developing drug action targets innovative treatment strategies.
Language: Английский
Citations
0
Journal of Biological Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108548 - 108548
Published: April 1, 2025
Liquid-liquid phase separation is a fundamental biophysical process in which biopolymers, such as proteins, nucleic acids, and their complexes, spontaneously demix into distinct coexisting phases. This phenomenon drives the formation of membraneless organelles-cellular subcompartments without lipid bilayer that perform specialized functions. In plants, phase-separated biomolecular condensates play pivotal roles regulating gene expression, from genome organization to transcriptional post-transcriptional processes. addition, governs plant-specific traits, flowering photosynthesis. As sessile organisms, plants have evolved leverage for rapid sensing response environmental fluctuations stress conditions. Recent studies highlight critical role plant adaptation, particularly abiotic stress. review compiles latest research on biology, providing examples diverse functions development, responses. We propose represents conserved dynamic mechanism enabling adapt efficiently ever-changing Deciphering molecular mechanisms underlying responses opens new avenues biotechnological strategies aimed at engineering stress-resistant crops. These advancements significant implications agriculture, addressing crop productivity face climate change.
Language: Английский
Citations
0
PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e19402 - e19402
Published: May 2, 2025
DNA repair is a hierarchically organized, spatially and temporally regulated process involving numerous factors that respond to various types of damage. Despite decades research, the mechanisms by which these are recruited depart from sites have been subject intrigue. Recent advancements in field increasingly highlighted role phase separation as critical facilitator efficiency repair. This review emphasizes how enhances concentration coordination at damage sites, optimizing efficiency. Understanding dysregulation can impair alter nuclear organization, potentially leading diseases such cancer neurodegenerative disorders, crucial. manuscript provides comprehensive understanding pivotal repair, sheds light on current suggests potential future directions for research therapeutic interventions.
Language: Английский
Citations
0
The Plant Cell, Journal Year: 2023, Volume and Issue: 35(9), P. 3345 - 3362
Published: June 19, 2023
Abstract Plants cope with various recurring stress conditions that often induce DNA damage, ultimately affecting plant genome integrity, growth, and productivity. The CROWDED NUCLEI (CRWN) family comprises lamin-like proteins multiple functions, such as regulating gene expression, organization, damage repair in Arabidopsis (Arabidopsis thaliana). However, the mechanisms consequences of CRWNs are largely unknown. Here, we reveal maintain stability by forming repairing nuclear bodies at double-strand breaks. We demonstrate CRWN1 CRWN2 physically associate RAD51D SUPPRESSOR OF NPR1-1 Inducible 1 (SNI1) act same genetic pathway to mediate this process. Moreover, partially localize γ-H2AX foci upon damage. Notably, undergo liquid–liquid phase separation form highly dynamic droplet-like structures SNI1 promote response (DDR). Collectively, our data shed light on function DDR maintenance stability.
Language: Английский
Citations
8
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown
Published: May 28, 2022
Abstract Mutation is the ultimate source of genetic variation. rate variability has been observed within plant genomes, but underlying mechanisms have unclear. We previously found that mutations occur less often in functionally constrained regions genome Arabidopsis thaliana and this mutation reduction predicted by H3K4me1, a histone modification gene bodies actively expressed evolutionarily conserved genes plants. reanalyzed de novo germline single base substitutions fast neutron irradiated accumulation lines Kitaake rice ( Oryza sativa ) same associated with bodies, as A. , suggesting for Here, we characterize model targeted DNA repair to explain these observations; PDS5C MSH6 repair-related proteins target H3K4me1 through their Tudor domains, resulting nearby experiencing elevated repair. Experimental data in-silico modeling support high affinity domain both proteins, between species. ChIP-seq from confirms its localization low regions. Somatic deep sequencing wild-type knockout confirm preferentially repairs H3K4me1-enriched These findings inspire further research origins eukaryotes consequences on tuning evolutionary trajectories genomes.
Language: Английский
Citations
14