Plum pox virus: An overview of the potyvirus behind sharka, a harmful stone fruit disease DOI Creative Commons
Juan Antonio Garcı́a, Bernardo Rodamilans, Sandra Martínez‐Turiño

et al.

Annals of Applied Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 4, 2024

Abstract The study of Potyvirus plumpoxi (plum pox virus, PPV) has a long history, beginning with its foundational description by Atanassof in 1932 Bulgaria. Interest the virus significantly increased over past decades, as evidenced rise published articles, highlighting importance plant pathology. Research on PPV offers broader insights into biology and pathology extensive Potyviridae family to which it belongs. virus's large strain diversity wide host range make key subject for research plant–virus interactions, adaptation evolution. causes sharka, most damaging disease affecting stone fruit trees. Economic losses from sharka have recently been estimated at €2.4 thousand million last 28 years. significance this is further amplified ease aphid‐mediated transmission infected material ability establish spread new regions, making global agricultural challenge. This review aims provide comprehensive overview various biological traits will form basis an updated version Association Applied Biologists Description Plant Viruses PPV.

Language: Английский

Plant virology in the 21st century in China: Recent advances and future directions DOI Creative Commons
Jianguo Wu, Yongliang Zhang, Fangfang Li

et al.

Journal of Integrative Plant Biology, Journal Year: 2023, Volume and Issue: 66(3), P. 579 - 622

Published: Nov. 4, 2023

Plant viruses are a group of intracellular pathogens that persistently threaten global food security. Significant advances in plant virology have been achieved by Chinese scientists over the last 20 years, including basic research and technologies for preventing controlling viral diseases. Here, we review these milestones advances, identification new crop-infecting viruses, dissection pathogenic mechanisms multiple examination multilayered interactions among their host plants, virus-transmitting arthropod vectors, in-depth interrogation plant-encoded resistance susceptibility determinants. Notably, various virus-based vectors also successfully developed gene function studies target expression plants. We recommend future China.

Language: Английский

Citations

52
The 6-kilodalton peptide 1 in plant viruses of the family Potyviridae is a viroporin DOI

Mengzhu Chai,

Lei Li, Yong Li

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(21)

Published: May 13, 2024

Potyviridae, the largest family of plant RNA viruses, includes many important pathogens that significantly reduce yields crops worldwide. In this study, we report 6-kilodalton peptide 1 (6K1), one least characterized potyviral proteins, is an endoplasmic reticulum–localized protein. AI–assisted structure modeling and biochemical assays suggest 6K1 forms pentamers with a central hydrophobic tunnel, can increase cell membrane permeability Escherichia coli Nicotiana benthamiana , conduct potassium in Saccharomyces cerevisiae . An infectivity assay showed viral proliferation inhibited by mutations affect multimerization. Moreover, or its homologous 7K proteins from other viruses Potyviridae also have ability to transmembrane conductance. Taken together, these data reveal function as viroporins infected cells.

Language: Английский

Citations

10
Chloroplast immunity: A cornerstone of plant defense DOI
Jie Liu, Pan Gong,

Ruo-bin Lu

et al.

Molecular Plant, Journal Year: 2024, Volume and Issue: 17(5), P. 686 - 688

Published: March 19, 2024

Language: Английский

Citations

9
Hidden viral proteins: How powerful are they? DOI Creative Commons
Fangfang Li,

Mingxuan Jia,

Aiming Wang

et al.

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(1), P. e1011905 - e1011905

Published: Jan. 18, 2024

Language: Английский

Citations

6
A conserved lysine/arginine-rich motif is essential for the autophagic degradation of potyviral 6K1 protein and virus infection DOI Creative Commons

Weiyao Hu,

Changhui Deng,

Qin Li

et al.

Journal of Virology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

ABSTRACT Potyviruses possess one positive-sense single-stranded RNA genome, mainly dependent on polyprotein processing as the expression strategy. The resulting polyproteins are proteolytically processed by three virus-encoded proteases into 11 or 12 mature proteins. One such factor, 6 kDa peptide 1 (6K1), is an understudied viral factor. Its function in infection remains largely mysterious. This study to reveal part of its roles using pepper veinal mottle virus (PVMV) model. Alanine substitution screening analysis revealed that 15 17 conserved residues across potyviral 6K1 sequences essential for PVMV infection. However, protein less accumulated virus-infected cells, although P3-6K1 and 6K1-CI junctions efficiently NIa-Pro release, indicating undergoes a self-degradation event. Mutating cleavage site prevent abolishes infection, suggesting generation along with degradation might be important multiplication. We corroborated cellular autophagy engaged 6K1’s degradation. Individual engineering variants allows their Five variants, D30A, V32A, K34A, L36A, L39A, significantly interfere five enclosed lysine/arginine-rich motif; four them appear crucial engaging autophagy-mediated self-degradation. Based these data, we envisaged scenario which 6K1s interact unknown anti-viral component co-degraded promote IMPORTANCE Potyvirus largest genus plant-infecting viruses, encompasses socio-economically species, Potato Y , Plum pox Soybean mosaic . Like all picorna-like potyviruses express factors via processing. Theoretically, P3 through CP, including 6K1, should share equivalent number molecules. small size (~6 kDa) but cells. demonstrates In particular, found motif this finding reveals facet helps understand pro-viral role

Language: Английский

Citations

0
A novel peptide encoded by a rice circular RNA confers broad‐spectrum disease resistance in rice plants DOI Open Access
Xin Pan, Sipei Xu, Gehui Cao

et al.

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Summary Circular RNAs (circRNAs) are a significant class of endogenous that exert crucial biological functions in human and animal systems, but little is currently understood regarding their roles plants. Here, we identified circRNA originating from the back‐splicing exon 4 5 rice gene, OsWRKY9 , named it circ‐WRKY9. It upregulated stripe mosaic virus (RSMV)‐infected Notably, circ‐WRKY9 contains two open reading frames with an internal ribosome entry site. We found encoded peptide 88 amino acids (aa) WRKY9‐88aa. Overexpression WRKY9‐88aa suppresses RSMV infection plants, increased reactive oxygen species production. Furthermore, enhances resistance to blast disease bacterial leaf blight, suggesting its potential provide broad‐spectrum resistance. Our findings first evidence by planta highlight application control wide spectrum plant diseases.

Language: Английский

Citations

0
Cucumber green mottle mosaic virus encodes additional small proteins with specific subcellular localizations and virulence function DOI
Pan Gong, Min Gao, Yalin Chen

et al.

Science China Life Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Construction of watermelon mosaic virus-Beijing isolate infectious clone and study of the dynamic localization and accumulation of virus-encoded proteins DOI

Mengjiao Pan,

Linhao Ge,

Weifang Gao

et al.

Virology, Journal Year: 2025, Volume and Issue: unknown, P. 110571 - 110571

Published: May 1, 2025

Language: Английский

Citations

0
ORF3c is expressed in SARS‐CoV‐2‐infected cells and inhibits innate sensing by targeting MAVS DOI Creative Commons
Martin Müller,

Alexandra Herrmann,

Shigeru Fujita

et al.

EMBO Reports, Journal Year: 2023, Volume and Issue: 24(12)

Published: Oct. 23, 2023

Most SARS-CoV-2 proteins are translated from subgenomic RNAs (sgRNAs). While the majority of these sgRNAs monocistronic, some viral mRNAs encode more than one protein. One example is ORF3a sgRNA that also encodes ORF3c, an enigmatic 41-amino-acid peptide. Here, we show ORF3c expressed in SARS-CoV-2-infected cells and suppresses RIG-I- MDA5-mediated IFN-β induction. interacts with signaling adaptor MAVS, induces its C-terminal cleavage, inhibits interaction RIG-I MAVS. The immunosuppressive activity conserved among members subgenus sarbecovirus, including SARS-CoV coronaviruses isolated bats. Notably, however, delta kappa variants harbor premature stop codons demonstrating this reading frame not essential for efficient replication vivo likely compensated by other proteins. In agreement this, disruption does significantly affect CaCo-2, CaLu-3, or Rhinolophus alcyone cells. summary, here identify as immune evasion factor innate sensing infected

Language: Английский

Citations

8
Deeper look into viruses: replication intermediates do code! DOI
Ashish Prasad, Shambhavi Sharma, Manoj Prasad

et al.

Plant Cell Reports, Journal Year: 2024, Volume and Issue: 43(2)

Published: Feb. 1, 2024

Language: Английский

Citations

1