核糖体介导的翻译调控与植物环境温度适应

Chinese Science Bulletin (Chinese Version), Journal Year: 2025, Volume and Issue: 70(7), P. 807 - 815

Published: Feb. 8, 2025

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Plant RNA-binding proteins: Phase separation dynamics and functional mechanisms underlying plant development and stress responses

Molecular Plant, Journal Year: 2024, Volume and Issue: 17(4), P. 531 - 551

Published: Feb. 27, 2024

RNA-binding proteins (RBPs) accompany RNA from synthesis to decay, mediating every aspect of metabolism and impacting diverse cellular developmental processes in eukaryotes. Many RBPs undergo phase separation along with their bound form function dynamic membraneless biomolecular condensates for spatiotemporal coordination or regulation metabolism. Increasing evidence suggests that phase-separating domains intrinsically disordered regions play important roles plant development stress adaptation. Here, we summarize the current knowledge about how partitioning into controls enables sensing experimental changes confer growth plasticity under conditions, a focus on dynamics functional mechanisms RBP-rich nuclear cytoplasmic granules We also discuss multiple factors, such as environmental signals, protein modifications, N

Language: Английский

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High-throughput affinity measurements of direct interactions between activation domains and co-activators

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 20, 2024

Abstract Sequence-specific activation by transcription factors is essential for gene regulation 1,2 . Key to this are domains, which often fall within disordered regions of 3,4 and recruit co-activators initiate 5 These interactions difficult characterize via most experimental techniques because they typically weak transient 6,7 Consequently, we know very little about whether these promiscuous or specific, the mechanisms binding, how tune strength activation. To address questions, developed a microfluidic platform expression purification hundreds domains in parallel followed direct measurement co-activator binding affinities (STAMMPPING, Simultaneous Trapping Affinity Measurements Microfluidic Protein-Protein INteraction Generator). By applying STAMMPPING quantify between eight 204 human (>1,500 K d s), provide first quantitative map reveal 334 novel pairs. We find that metazoan-specific P300 directly binds >100 potentially explaining its widespread recruitment across genome influence transcriptional Despite sharing similar molecular properties ( e.g. enrichment negative hydrophobic residues), utilize distinct biophysical certain domains. Co-activator domain affinity occupancy well-predicted analytical models account multivalency, vitro quantitatively predict cells with an ultrasensitive response. Not only do our results demonstrate ability measure even protein-protein high throughput, but also necessary resource over 1,500 domain/co-activator lays foundation understanding basis

Language: Английский

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The glycine-rich domain of GRP7 plays a crucial role in binding long RNAs and facilitating phase separation

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 11, 2024

Abstract Microscale thermophoresis (MST) is a well-established method to quantify protein-RNA interactions. In this study, we employed MST analyze the RNA binding properties of glycine-rich protein 7 (GRP7), which known have multiple biological functions related its ability bind different types RNA. However, exact mechanism GRP7’s not fully understood. While RNA-recognition motif GRP7 be involved in binding, region (known as arginine-glycine-glycine-domain or RGG-domain) also influences interaction. To investigate extend RGG-domain mutation studies on putative interacting modulating sites were performed. addition experiments, examined liquid–liquid phase separation and mutants, both with without Furthermore, systemically investigated factors that might affect selectivity by testing RNAs sizes, structures, modifications. Consequently, our study revealed exhibits high affinity for variety RNAs, indicating lack pronounced selectivity. Moreover, established plays crucial role longer promoting separation.

Language: Английский

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High temperature‐responsive DEAR4 condensation confers thermotolerance through recruiting TOPLESS in Arabidopsis nucleus

The Plant Journal, Journal Year: 2025, Volume and Issue: 122(2)

Published: April 1, 2025

SUMMARY Global warming is harmful to plants and threatens crop yields in the world. In contrast other abiotic stresses, molecular mechanisms for plant high temperature perception signaling are still not fully understood. Here, we report that transcription factor DREB AND EAR MOTIF PROTEIN 4 (DEAR4) positively regulates heat tolerance Arabidopsis thaliana . We further reveal DEAR4 proteins undergo liquid–liquid phase separation (LLPS) could induce condensate formation nucleus. Moreover, recruits transcriptional co‐repressor TOPLESS (TPL) into nuclear speckles under temperature. The triggered DEAR4‐TPL co‐condensates enhance their repression activity through modulating histone deacetylation levels of GASA5 , which a reported negative regulator HEAT SHOCK PROTEINs ( HSPs ). A genome‐wide landscape study confirms induces expression multiple Taken together, illustrate mechanism mediated by LLPS confer thermotolerance open new avenue translating this knowledge crops improving resistance.

Language: Английский

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ALBA3 maintains male fertility under heat stress in plants

Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

Heat stress (HS) at the reproductive stage detrimentally affects crop yields and seed quality. However, molecular mechanisms that protect processes in plants under HS remain largely unknown. Here, we report Acetylation Lowers Binding Affinity 3 (ALBA3) is crucial for safeguarding male fertility against Arabidopsis. ALBA3 highly expressed pollen, localized cytoplasm of both sperm vegetative cells. Mutants lacking functional exhibit hypersensitivity to HS, with reduced silique length due defects pollen germination, pollination, tube growth, fertilization HS. binds stabilizes a subset messenger RNAs (mRNAs) essential function, thereby protecting fertility. Two residues Alba domain, K46 L90, are critical ALBA3's ability bind stabilize mRNAs necessary its proper function. Interestingly, loss rice also leads severe abortion sterility highlighting conserved role across plant species. This study uncovers mechanism by which safeguards during stabilizing specific

Language: Английский

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RALF-FER, a master ligand‒receptor pair in plant health

Crop Health, Journal Year: 2025, Volume and Issue: 3(1)

Published: Feb. 20, 2025

Language: Английский

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Phase separation as a key mechanism in plant development, environmental adaptation, and abiotic stress response

Journal of Biological Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108548 - 108548

Published: April 1, 2025

Liquid-liquid phase separation is a fundamental biophysical process in which biopolymers, such as proteins, nucleic acids, and their complexes, spontaneously demix into distinct coexisting phases. This phenomenon drives the formation of membraneless organelles-cellular subcompartments without lipid bilayer that perform specialized functions. In plants, phase-separated biomolecular condensates play pivotal roles regulating gene expression, from genome organization to transcriptional post-transcriptional processes. addition, governs plant-specific traits, flowering photosynthesis. As sessile organisms, plants have evolved leverage for rapid sensing response environmental fluctuations stress conditions. Recent studies highlight critical role plant adaptation, particularly abiotic stress. review compiles latest research on biology, providing examples diverse functions development, responses. We propose represents conserved dynamic mechanism enabling adapt efficiently ever-changing Deciphering molecular mechanisms underlying responses opens new avenues biotechnological strategies aimed at engineering stress-resistant crops. These advancements significant implications agriculture, addressing crop productivity face climate change.

Language: Английский

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Unveiling the regulatory role of GRP7 in ABA signal-mediated mRNA translation efficiency regulation

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 26, 2025

Abscisic acid (ABA) is a crucial phytohormone involved in plant growth and stress responses. While the transcriptional regulation triggered by ABA well-documented, its effects on translational have been less studied. Through Ribo-seq RNA-seq analyses, we find that treatment not only influences gene expression at mRNA level but also significantly impacts translation efficiency (TE) Arabidopsis thaliana. inhibits global via core signaling pathway, which includes receptors, protein phosphatase 2Cs (PP2Cs), SNF1-related kinase 2 s (SnRK2s). Upon treatment, Glycine-rich RNA-binding proteins 7 8 (GRP7&8) levels decrease due to both reduced decreased TE, diminishes their association with polysomes leads decline TE. The absence of GRP7&8 results impairment ABA-regulated changes, linking GRP7-dependent modulation translation. GRP7 TE relies direct binding target mRNAs. Moreover, under drought partially dependent ABA-GRP7&8 pathways. Collectively, our study reveals GRP7's role downstream SnRK2s mediating signaling, offering model for ABA-triggered multi-route environmental adaptation.

Language: Английский

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Identification of uranyl-binding proteins in Arabidopsis thaliana cells exposed to uranium: Insights from a metalloproteomic analysis and characterization of Glycine-Rich RNA-binding Protein 7 (GRP7)

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Abstract Uranium (U) is a naturally occurring radionuclide, chemotoxic for living organisms. To identify proteins that could be cellular targets U in plants, we used metalloproteomic approaches combining column chromatographic fractionation analyses, protein identification by high-resolution mass spectrometry shotgun proteomics, and metal quantification induced coupled plasma spectrometry. We identified 57 candidate uranyl (U(VI)) binding cultured Arabidopsis thaliana cells. One of these proteins, the Glycine-Rich RNA-binding Protein 7 (GRP7) involved various developmental processes responses to biotic abiotic stress. Recombinant GRP7 was purified from overproducing bacteria subjected further biochemical characterization. First, showed binds U(VI) with 1:2 (protein:metal) stoichiometry vitro . Next, analyzed its structural properties solution-state nuclear magnetic resonance spectroscopy. This allowed us gain insight into molecular dynamics protein-metal interaction residues U(VI)-binding two sites. Finally, observed interferes nucleic acid domain, suggesting contributes toxicity plants. HIGHLIGHTS uranyl-binding were cells exposed uranyl, which GRP7. In , recombinant 2 ions within RNA recognition motif domain. Amino both sites identified. Competition between specific oligonucleotide suggests implication uranium toxicity, as target.

Language: Английский

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