Blood transcriptomics identifies FEZ1 as a novel non-invasive diagnostic biomarker for inflammatory bowel disease DOI
Arman Mokaram Doust Delkhah

Published: Oct. 28, 2024

Abstract In comparison with conventional diagnostic methods, blood biomarkers are an easily accessible source for diagnosing inflammatory bowel disease (IBD). To identify such a biomarker, integrated transcriptomics approach was employed to detect RNAs exhibiting efficacy. For this purpose, four gene expression datasets obtained from specimens were (GSE119600, GSE94648, GSE86434, and GSE71730). After determining differentially expressed genes in Crohn’s ulcerative colitis, DEGs IBD defined as consistent direction of alteration both disorders. Mapping the PPI network these revealed TNF central hub gene. Subsequently, weighted gene-expression analysis (WGCNA) carried out determine IBD-specific modules. Considering degree metrics, module membership, significance, PRF1 only discerned that prominently enriched IBD. Genes converging results differential WGCNA subjected random forest decision tree-based LASSO regression methods. Following identification FEZ1 NLRC5 highlighted by analyses, ROC applied assess their potential. Although demonstrated acceptable efficacy data, considered potential biomarker based on replication validation While autophagy is currently most convincing explanation involvement IBD, further investigations required elucidate its immunological role.

Language: Английский

Association between silicosis and autoimmune disease DOI Creative Commons
Elizabeth Fireman, Einat Fireman Klein

Current Opinion in Allergy and Clinical Immunology, Journal Year: 2024, Volume and Issue: 24(2), P. 45 - 50

Published: Jan. 24, 2024

Purpose of review There is a well established association between silica inhalational exposure and autoimmune disease, particularly in the context intense exposure. We will provide this article an update overview new sources dust exposure, with evidences mechanisms from human animal studies for diseases, their early detection silicosis options treatment. Recent findings New industries such as jewelry polishing, denim jean production, fabrication artificial stone benchtops, glass manufacturing glassware has led to re-emergence around world. Silicosis long term containing crystalline been examined possible risk factor respect several diseases scleroderma, rheumatoid arthritis, lupus erythematosus, some types small vessel vasculitis renal involvement. The may act promote or accelerate disease development, requiring other factors break immune tolerance initiate autoimmunity. Autophagy, apoptosis, pyroptosis-related signaling pathways have also suggested contribute formation those coordination environmental co-exposure that can magnify vulnerability. Summary Better understanding involve -induced diagnosis.

Language: Английский

Citations

5
Blood transcriptomics identifies FEZ1 as a potential biomarker for inflammatory bowel disease DOI
Arman Mokaram Doust Delkhah

Computers in Biology and Medicine, Journal Year: 2025, Volume and Issue: 187, P. 109742 - 109742

Published: Feb. 1, 2025

Language: Английский

Citations

0
Herpes Simplex Virus type 1 inhibits autophagy in glial cells but requires ATG5 for the success of viral replication DOI Creative Commons
Inés Ripa, Sabina Andreu, Fernando Josa‐Prado

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: June 10, 2024

Herpes Simplex Virus type 1 (HSV-1) is a neurotropic virus that has been associated with neurodegenerative disorders. The dysregulation of autophagy by HSV-1 proposed as potential cause neurodegeneration. While studies have extensively tackled the interaction between and in neurons, research glial cells currently limited. Our demonstrate inhibits, but not completely blocks, formation autophagosomes human oligodendroglioma- astrocytoma- derived cell lines. These findings confirmed murine oligodendrocyte precursor (OPCs). Finally, this study investigates impact on infection cells. lack basal LC3B knockout does significant effect viral infection, without autophagy-related protein ATG5 exhibit reduced production. absence leads to decrease transcription replication genes, well delay initial stages compartments. indicate while may play role antiviral defense cells, be inhibiting exploit non-canonical functions certain components autophagic machinery, such ATG5, benefit its lifecycle.

Language: Английский

Citations

2
Blood transcriptomics identifies FEZ1 as a novel non-invasive diagnostic biomarker for inflammatory bowel disease DOI
Arman Mokaram Doust Delkhah

Published: Oct. 28, 2024

Abstract In comparison with conventional diagnostic methods, blood biomarkers are an easily accessible source for diagnosing inflammatory bowel disease (IBD). To identify such a biomarker, integrated transcriptomics approach was employed to detect RNAs exhibiting efficacy. For this purpose, four gene expression datasets obtained from specimens were (GSE119600, GSE94648, GSE86434, and GSE71730). After determining differentially expressed genes in Crohn’s ulcerative colitis, DEGs IBD defined as consistent direction of alteration both disorders. Mapping the PPI network these revealed TNF central hub gene. Subsequently, weighted gene-expression analysis (WGCNA) carried out determine IBD-specific modules. Considering degree metrics, module membership, significance, PRF1 only discerned that prominently enriched IBD. Genes converging results differential WGCNA subjected random forest decision tree-based LASSO regression methods. Following identification FEZ1 NLRC5 highlighted by analyses, ROC applied assess their potential. Although demonstrated acceptable efficacy data, considered potential biomarker based on replication validation While autophagy is currently most convincing explanation involvement IBD, further investigations required elucidate its immunological role.

Language: Английский

Citations

0