Optimizing solvent systems for electrospun PLGA scaffolds: effects on microstructure and mechanical properties for biomedical applications

RSC Advances, Journal Year: 2025, Volume and Issue: 15(5), P. 3259 - 3272

Published: Jan. 1, 2025

Optimizing solvent systems for electrospun PLGA tissue scaffolds enables control over mechanical properties. Hansen solubility theory helps identify key effects, enabling the design of tunable, high-performance biomedical use.

Language: Английский

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Light-Blocking Nanofiber Membranes Facilitating Physiologically Relevant In Situ Transmigration Assay

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

Nanofiber (NF) membranes have demonstrated considerable potential in cellular transmigration studies due to their resemblance the biophysical properties of basement membranes, enabling behaviors that closely mimic those observed vivo. Despite advantages, conventional NF often encounter issues assays transparency, which leads overlapping fluorescent signals from transmigrated and nontransmigrated cells. This overlap complicates clear differentiation between these cell populations, making quantitative evaluation live-cell challenging. To address this issue, we developed a light-blocking nanofiber (LB-NF) membrane by incorporating carbon black into polycaprolactone NFs. LB-NF is designed not only but also enable situ analysis cells through its properties. Our study effectiveness assay using human brain cerebral microvascular endothelial (HBEC-5i), physiologically relevant while significantly enhancing accuracy fluorescence detection. Furthermore, drug testing within choroidal neovascularization model underscores utility impact on pharmaceutical development, particularly for diseases involving abnormal transmigration. Therefore, represents valuable tool precise assessment holds significant promise advancing screening therapeutic development.

Language: Английский

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Harnessing the Underutilized Potential of Lens Capsule Transplantation in Ophthalmology: A Narrative Review of Current Applications and Future Directions

Cureus, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

The human lens capsule is a transparent and durable basement membrane routinely discarded during cataract surgery, exhibiting unique biochemical, biomechanical, immunologic properties. This narrative review discusses validated ophthalmic applications across corneal, retinal, glaucoma filtration surgeries. It emphasizes the capsule's emerging role as tissue-engineering scaffold for cultivating corneal endothelial cells, limbal epithelial stem retinal pigment demonstrating significant promise in regenerative ophthalmology. However, variability harvesting techniques, small graft sizes, limited long-term clinical data currently hinder its broader implementation. Future directions highlight necessity of standardizing preservation protocols, potentially collaboration with eye banks, to enhance accessibility utility. Additionally, this explores speculative applications, including encapsulation devices drug cell delivery, ultraviolet cross-linking keratoconus management, novel scaffolds optic nerve regeneration transplantation. While preliminary evidence strongly supports versatility, rigorous trials comparative analyses remain essential establish safety, efficacy, optimal surgical integration. Ultimately, harnessing naturally available biomaterial represents meaningful advancement ophthalmology, opening new horizons future research.

Language: Английский

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Kidney-on-a-Chip: Mechanical Stimulation and Sensor Integration

Sensors, Journal Year: 2022, Volume and Issue: 22(18), P. 6889 - 6889

Published: Sept. 13, 2022

Bioengineered in vitro models of the kidney offer unprecedented opportunities to better mimic vivo microenvironment. Kidney-on-a-chip technology reproduces 2D or 3D features which can replicate tissue architecture, composition, and dynamic mechanical forces experienced by cells vivo. Kidney are exposed stimuli such as substrate stiffness, shear stress, compression, stretch, regulate multiple cellular functions. Incorporating kidney-on-a-chip is critically important for recapitulating physiological pathological This review will explore approaches applying different cell types using how these systems used study physiology, model disease, screen drug toxicity. We further discuss sensor integration into monitoring responses other stimuli. advantages, limitations, challenges associated with incorporating a variety applications. Overall, this aims highlight importance design implementation devices.

Language: Английский

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A layered cancer-on-a-chip system for anticancer drug screening and disease modeling

The Analyst, Journal Year: 2023, Volume and Issue: 148(21), P. 5486 - 5495

Published: Jan. 1, 2023

Recent advances in the development of microfluidic systems for culture complex and three-dimensional cell, tissue, organ models allow their use toxicity studies mimicking many diseases. These types

Language: Английский

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Construction of in vitro liver-on-a-chip models and application progress

BioMedical Engineering OnLine, Journal Year: 2024, Volume and Issue: 23(1)

Published: March 15, 2024

Abstract The liver is the largest internal organ of human body. It has a complex structure and function plays vital role in drug metabolism. In recent decades, extensive research aimed to develop vitro models that can simulate demonstrate changes physiological pathological environment liver. Animal cell are common, but data obtained from animal lack relevance when applied humans, while have limited predictive ability for metabolism toxicity humans. Recent advancements tissue engineering, biomaterials, chip technology, 3D bioprinting provided opportunities further models. Among them, liver-on-a-Chip (LOC) technology made significant achievements reproducing vivo behavior, microenvironment, cells organs. this review, we discuss development LOC its progress diseases, hepatotoxicity tests, screening, as well combinations. First, review Then, introduce including general concepts, preparation materials, methods. Finally, application disease modeling, combinations, future challenges directions LOC.

Language: Английский

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Fluid flow to mimic organ function in 3D in vitro models

APL Bioengineering, Journal Year: 2023, Volume and Issue: 7(3)

Published: Aug. 4, 2023

Many different strategies can be found in the literature to model organ physiology, tissue functionality, and disease

Language: Английский

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Development of an Induced Pluripotent Stem Cell-Based Liver-on-a-Chip Assessed with an Alzheimer’s Disease Drug

ACS Biomaterials Science & Engineering, Journal Year: 2023, Volume and Issue: 9(7), P. 4415 - 4430

Published: June 15, 2023

Liver-related drug metabolism is a key aspect of pharmacokinetics and possible toxicity. From this perspective, the availability advanced in vitro models for testing still an open need, also to end reducing burden vivo experiments. In scenario, organ-on-a-chip gaining attention as it couples state-of-the art approach recapitulation physiological features such fluidodynamics tri-dimensional cytoarchitecture. We implemented novel liver-on-a-chip (LoC) device based on innovative dynamic (MINERVA 2.0) where functional hepatocytes (iHep) have been encapsulated into 3D hydrogel matrix interfaced through porous membrane with endothelial cells (iEndo)]. Both lines were derived from human-induced pluripotent stem (iPSCs), LoC was functionally assessed donepezil, approved Alzheimer's disease therapy. The presence iEndo microenvironment enhanced expression liver-specific physiologic functions iHep, after 7 day perfusion, we noticed increase albumin, urea production, cytochrome CYP3A4 compared iHep static culture. particular, donepezil kinetics, computational fluid study conducted assess amount diffused indicated that molecule should be able pass reach target construct. Then, performed experiments kinetics confirmed numerical simulations. Overall, our iPSC-based reproduced liver suitable potential hepatotoxic screening studies.

Language: Английский

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Recent frontiers in biofabrication for respiratory tissue engineering

Bioprinting, Journal Year: 2024, Volume and Issue: 40, P. e00342 - e00342

Published: April 21, 2024

Respiratory tissue engineering offers a robust framework for studying cell-cell and host-pathogen interactions in tissue-like environment platform lung regeneration disease mechanisms. However, the challenge of replicating dynamic three-dimensional (3D) microenvironments is huge obstacle with existing technology. Current animal models two-dimensional cell culture do not replicate vivo conditions seen human lungs, thus research utilizing these techniques often fails to help alleviate global burden respiratory diseases. has been drawing significant attention over past decade. Particularly emergence severe acute syndrome coronavirus 2 (SARS-CoV-2), many inspiring developments advances have reported. This review presents recent focusing on 3D bioprinting, organ-on-a-chip, organoid technologies. It also provides an overview attempts integrate biomechanical stimulus aim improving integrity constructs enhancing cellular propagation. addresses challenges inherent discusses future prospects this field, urging continuing innovation investment toward success increasing clinical relevance.

Language: Английский

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Development of an in vitro platform for epithelial-stromal interactions: A basement membrane-containing scaffold from decellularized porcine bladders

Matrix Biology Plus, Journal Year: 2025, Volume and Issue: 26, P. 100169 - 100169

Published: Feb. 22, 2025

Language: Английский

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