bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 31, 2025
Abstract
Extracellular
vesicles
(EVs)
are
versatile
transporters
of
genetic
cargo
with
enormous
potential
in
the
therapeutic
setting.
Scalable
production
EVs,
and
routes
to
overcome
rapid
clearance
required.
Biocompatible
hydrogels
may
support
precise,
localized
delivery
EVs
target
sites.
This
study
aimed
establish
sustained
a
scalable
3D
dynamic
bioreactor
fabricate
using
tyramine-modified
hyaluronic
acid
(HA-TA)
EV
integration
release
patterns.
MDA-MB-231
cells
transduced
lentiviral
GFP
fused
CD63,
were
cultured
20kD
hollow
fiber
GFP-EVs
harvested
over
five
weeks.
characterized
by
Nanoparticle
Tracking
Analysis(NTA),
Western
Blot(WB)
Transmission
Electron
Microscopy(TEM).
Tyramine
modified
acid(HA-TA)
formulated
via
enzymatic
crosslinking
hydrogen
peroxide
horseradish
peroxidase,
investigate
patterns
static
conditions.
Hydrogel
swelling
was
recorded
at
1-72
hrs
loaded
assess
distribution
Scanning
Microscopy(SEM)
NTA
respectively.
GFP-EV
uptake
assessed
confocal
microscopy.
Longitudinal
expression
demonstrated
released
throughout
culture.
TEM
successful
isolation
30-200
nm
size
intact
lipid
bilayers
(average
4×10
9
EVs/harvest).
Initial
harvests
exhibited
subpopulations
larger
which
disappeared
upon
serum
withdrawal.
WB
verified
presence
markers
TSG101,
CD81.
HA-TA
successfully
formed
assays
revealed
requirement
for
higher
concentrations
crosslinkers
scaffold
stability
continued
swelling.
incorporated
into
variable
observed
time,
depending
on
concentration
hydrogel
formulation.
clusters
visualized
SEM.
Investigation
under
conditions
highlighted
significant
increase
fluid
flow
Efficient
transfer
recipient
also
vitro
.
The
data
demonstrate
engineered
free
subsequent
incorporation
release.
These
biocompatible
hold
promise
tuneable
variety
disease
settings.
Journal of Biomedical Materials Research Part B Applied Biomaterials,
Journal Year:
2024,
Volume and Issue:
112(1)
Published: Jan. 1, 2024
Abstract
This
study
explores
the
use
of
in
situ
cross‐linked
hyaluronic
acid
methacryloyl
(HAMA)
and
hydroxyapatite
particles
(HAP)
for
bone
defect
repair.
Human
periodontal
ligament
stem
cells
(PDLSCs)
were
isolated
co‐cultured
with
HAMA‐HAP
composite.
Osteogenic
differentiation
was
evaluated
using
Alizarin
Red
staining,
alkaline
phosphatase
activity
quantification,
polymerase
chain
reaction
(PCR).
A
cranial
induced
Sprague–Dawley
rats.
then
filled
composite
UV
light
exposure.
Bone
formation
assessed
through
radiographic
histological
analyses.
The
found
to
promote
cell
viability
similarly
pure
HAP.
It
also
enhanced
gene
expression
ALP,
OPN,
Runx2,
increased
ALP
mineralized
nodule
vitro.
Micro‐CT
scans
showed
restoration
HAP
groups
compared
control
group.
group
exhibited
higher
Tb.N,
Tb.Sp,
Tb.Th,
BV/TV.
Masson
staining
restored
site,
new
thicker
than
excellent
biocompatibility
promoted
osteogenic
PDLSCs.
effectively
repaired
defects,
indicating
its
potential
clinical
The FASEB Journal,
Journal Year:
2025,
Volume and Issue:
39(1)
Published: Jan. 10, 2025
Abstract
The
therapeutic
potential
of
extracellular
vesicles
(EVs)
in
bone
regeneration
is
noteworthy;
however,
their
clinical
application
impeded
by
low
yield
and
limited
efficacy.
This
study
investigated
the
effect
low‐intensity
pulsed
ultrasound
(LIPUS)
on
efficacy
EVs
derived
from
periodontal
ligament
stem
cells
(PDLSCs)
preliminarily
explored
its
mechanism.
PDLSCs
were
cultured
with
osteogenic
media
stimulated
or
without
LIPUS,
then
LIPUS‐stimulated
(L‐EVs)
isolated
separately.
We
biological
characteristics
effects
these
two
cell
proliferation,
migration,
differentiation,
vivo
vitro,
mechanism
analyzing
protein
profiles.
LIPUS
significantly
secretion
PDLSCs‐EVs,
L‐EVs
exhibited
stronger
promoting
thereby
enhancing
new
formation.
stimulation
affected
profile
42
proteins
upregulated
4
downregulated
when
compared
EVs.
level
cartilage
oligomeric
matrix
(COMP)
EVs,
which
enhanced
EVs'
ability
via
PI3K/AKT
pathway.
proposes
that
has
as
an
optimization
method
for
tissue
regeneration.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 31, 2025
Abstract
Extracellular
vesicles
(EVs)
are
versatile
transporters
of
genetic
cargo
with
enormous
potential
in
the
therapeutic
setting.
Scalable
production
EVs,
and
routes
to
overcome
rapid
clearance
required.
Biocompatible
hydrogels
may
support
precise,
localized
delivery
EVs
target
sites.
This
study
aimed
establish
sustained
a
scalable
3D
dynamic
bioreactor
fabricate
using
tyramine-modified
hyaluronic
acid
(HA-TA)
EV
integration
release
patterns.
MDA-MB-231
cells
transduced
lentiviral
GFP
fused
CD63,
were
cultured
20kD
hollow
fiber
GFP-EVs
harvested
over
five
weeks.
characterized
by
Nanoparticle
Tracking
Analysis(NTA),
Western
Blot(WB)
Transmission
Electron
Microscopy(TEM).
Tyramine
modified
acid(HA-TA)
formulated
via
enzymatic
crosslinking
hydrogen
peroxide
horseradish
peroxidase,
investigate
patterns
static
conditions.
Hydrogel
swelling
was
recorded
at
1-72
hrs
loaded
assess
distribution
Scanning
Microscopy(SEM)
NTA
respectively.
GFP-EV
uptake
assessed
confocal
microscopy.
Longitudinal
expression
demonstrated
released
throughout
culture.
TEM
successful
isolation
30-200
nm
size
intact
lipid
bilayers
(average
4×10
9
EVs/harvest).
Initial
harvests
exhibited
subpopulations
larger
which
disappeared
upon
serum
withdrawal.
WB
verified
presence
markers
TSG101,
CD81.
HA-TA
successfully
formed
assays
revealed
requirement
for
higher
concentrations
crosslinkers
scaffold
stability
continued
swelling.
incorporated
into
variable
observed
time,
depending
on
concentration
hydrogel
formulation.
clusters
visualized
SEM.
Investigation
under
conditions
highlighted
significant
increase
fluid
flow
Efficient
transfer
recipient
also
vitro
.
The
data
demonstrate
engineered
free
subsequent
incorporation
release.
These
biocompatible
hold
promise
tuneable
variety
disease
settings.
