Materials Today Bio, Journal Year: 2024, Volume and Issue: 28, P. 101226 - 101226
Published: Sept. 6, 2024
Language: Английский
Experimental & Molecular Medicine, Journal Year: 2024, Volume and Issue: 56(4), P. 836 - 849
Published: April 1, 2024
Abstract Exosomes, which are nanosized vesicles secreted by cells, attracting increasing interest in the field of biomedical research due to their unique properties, including biocompatibility, cargo loading capacity, and deep tissue penetration. They serve as natural signaling agents intercellular communication, inherent ability carry proteins, lipids, nucleic acids endows them with remarkable therapeutic potential. Thus, exosomes can be exploited for diverse applications, chemotherapy, gene therapy, photothermal therapy. Moreover, capacity homotypic targeting self-recognition provides opportunities personalized medicine. Despite advantages novel agents, there several challenges optimizing efficiency structural stability defining exosome origins. Future should include development large-scale, quality-controllable production methods, refinement drug strategies, extensive vivo studies clinical trials. unresolved difficulties, use efficient, stable, safe delivery systems is an interesting area research. Therefore, this review describes summarizes cutting-edge published high-impact journals that have introduced or enhanced effects using a system past 2 years. We provide informative overview current state research, highlighting properties applications exosomes. also emphasize future directions, underscoring importance addressing key issues field. With review, we encourage researchers further develop exosome-based drugs application, such may among most promising next-generation therapeutics.
Language: Английский
Citations
80
Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)
Published: March 27, 2024
Abstract Musculoskeletal disorders are the leading causes of physical disabilities worldwide. The poor self-repair capacity musculoskeletal tissues and absence effective therapies have driven development novel bioengineering-based therapeutic approaches. Adipose-derived stem cell (ADSC)-based being explored as new regenerative strategies for repair regeneration bone, cartilage, tendon owing to accessibility, multipotency, active paracrine activity ADSCs. In this review, recent advances in ADSCs their optimization strategies, including ADSC-derived exosomes (ADSC-Exos), biomaterials, genetic modifications, summarized. Furthermore, preclinical clinical applications ADSC-Exos, either alone or combination with growth factors biomaterials genetically modified forms, reviewed. ADSC-based hold promise management multiple types injuries. timely summary highlights provided here could offer guidance further investigations accelerate application regeneration. Graphical abstract
Language: Английский
Citations
17
International Journal of Molecular Medicine, Journal Year: 2025, Volume and Issue: 55(3)
Published: Jan. 8, 2025
Exosomes are integral to the pathophysiology of osteoarthritis (OA) due their roles in mediating intercellular communication and regulating inflammatory processes. transport bioactive molecules, such as proteins, lipids nucleic acids, which can influence chondrocyte behavior joint homeostasis. Given properties regeneration ability target damaged tissues, exosomes represent a promising therapeutic avenue for OA treatment. have potential promoting cartilage repair, reducing inflammation improving overall function. However, several challenges remain, including need standardized isolation characterization methods, variability exosomal content, regulatory hurdles. The present review aims provide comprehensive overview current understanding exosome mechanisms potential, while also addressing ongoing faced translating these findings into clinical practice. By consolidating existing research, pave way future studies aimed at optimizing exosome‑based therapies effective management.
Language: Английский
Citations
2
Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(30)
Published: May 1, 2024
Abstract Osteoarthritis (OA), a prevalent joint disease affecting many people globally, presents significant challenge in current treatments, which often only manage symptoms without halting progression. This review illuminates novel approaches OA therapy, focusing mainly on intra‐articular injection of nanomaterials. The innovative materials, designed to either mimic or augment natural lubrication, show promise restoring biomechanics and alleviating pain. delves into an array biomimetic lubricants, including polymer brush, nanocomposite hydrogel, nanoparticles, underscoring their roles anti‐inflammation, targeting, cartilage repair, drug delivery. Furthermore, the potential mesenchymal stem cells differentiate chondrocytes, coupled with delivery these exosomes via nanomaterials, has promoted cell therapy avenues for OA. also highlights function non‐coding RNAs such as miRNA, siRNA, circRNA, lncRNA, antisense oligonucleotides impeding progression, nanomaterials facilitating delivery, thus advanced therapeutic possibilities immune evasion bone proliferation. Overall, this encapsulates evolution treatment from material cell, ultimately gene forecasting future where evolve toward integrated, personalized diagnostics therapeutics
Language: Английский
Citations
13
Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(2), P. 147 - 147
Published: Jan. 22, 2025
Exosomes have emerged as promising therapeutic agents in regenerative medicine. This review introduces a novel cell type-oriented perspective to systematically analyze exosomal properties therapies. To our knowledge, this is the first comprehensively compare exosomes based on cellular source type, offering unprecedented insights into selecting optimal exosome producers for targeted applications. Factors beyond origin influencing efficacy, such donor sites and collection methods, are also explored here. By synthesizing key advances, we propose research directions end. We aim accelerate development of more effective exosome-based therapies highlight underexplored rapidly evolving field.
Language: Английский
Citations
1
Biochemical Pharmacology, Journal Year: 2024, Volume and Issue: 228, P. 116226 - 116226
Published: April 23, 2024
Osteoarthritis (OA) is a degenerative joint condition that contributes to years lived with disability. Current therapeutic approaches are limited as there no disease-modifying interventions able delay or inhibit the progression of disease. In recent has been an increasing interest in immunomodulatory and regenerative properties mesenchymal stem/stromal cells (MSCs) develop new OA therapies. Extracellular vesicles (EVs) mediate many biological effects these may represent alternative avoiding limitations cell-based therapy. There also growing EV modifications enhance their efficacy applications. Recent preclinical studies have provided strong evidence supporting potential MSC EVs for development treatments. Thus, regulate chondrocyte functions avoid cartilage destruction, abnormal subchondral bone metabolism synovial tissue alterations, control pain behavior. actions be mediated by transfer cargo target cells, important role proteins non-coding RNAs modulating signaling pathways relevant progression. Nevertheless, additional investigations needed concerning optimization, standardization preparation procedures. More research required better knowledge possible on different phenotypes, pharmacokinetics, mechanism action, long-term safety profile. Furthermore, high vehicles drug delivery adjuvant therapy potentiate complement other approaches.
Language: Английский
Citations
6
Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 179, P. 117364 - 117364
Published: Sept. 2, 2024
Osteoarthritis (OA) is a progressive degenerative disease resulting in joint deterioration. It whole organ characterized by cartilage degeneration and varying degrees of synovitis, involving pathological changes all tissues, such as cartilage, subchondral bone, ligaments, meniscus, synovium, infrapatellar fat pad (IPFP). IPFP the largest adipose tissue structure knee composed cells, immune cells blood vessels. Moreover, located close to bone surface so that it may reduce impact loading absorb forces generated through joint, have protective role health. has been shown release various cytokines adipokines play pro-inflammatory pro-catabolic roles promoting OA progression. Intra-articular injections IPFP-derived mesenchymal stem exosomes pain prevent progression patients with OA. Previous studies biphasic effect on This article reviews latest research progress IPFP, discusses mechanism OA, provide new intervention strategies for treatment will also discuss handling during procedure total arthroplasty.
Language: Английский
Citations
5
Materials Today Bio, Journal Year: 2024, Volume and Issue: 25, P. 100967 - 100967
Published: Jan. 21, 2024
Limb ischemia is a refractory disease characterized by persistent inflammation, insufficient angiogenesis, and tissue necrosis. Although mesenchymal stem cells (MSCs) have shown potential for treating limb ischemia, their therapeutic effects are limited low engraftment rates. Therefore, developing an optimal MSC delivery system that enhances cell viability imperative. Selenium, known its cytoprotective properties in various types, offers strategy to enhance effect of MSCs. In this study, we evaluated the selenium on MSCs, developed injectable thermosensitive selenium-containing hydrogel based PLGA-PEG-PLGA triblock copolymer, as carrier improve after engraftment. The biocompatibility, biodegradability, capabilities hydrogels were assessed. Furthermore, MSCs encapsulated within was using cellular animal experiments. Selenium protects from oxidative damage upregulating GPX4 through transcriptional mechanism. exhibited favorable antioxidant properties. It can be easily injected into target area liquid form at room temperature undergoes gelation body temperature, thereby preventing diffusion promoting cytoprotection effectively inhibited macrophage M1 polarization while M2 polarization, thus accelerating angiogenesis restoring blood perfusion ischemic limbs. This study demonstrated promising method delivery. By addressing challenge retention rate, which major obstacle application, improves ischemia.
Language: Английский
Citations
4
BMC Veterinary Research, Journal Year: 2025, Volume and Issue: 21(1)
Published: Jan. 21, 2025
Small extracellular vesicles (sEVs) derived from mesenchymal stem cells (MSCs) are recognized for their therapeutic potential in immune modulation and tissue repair, especially veterinary medicine. This study introduces an innovative sequential stimulation (IVES) technique, involving low-oxygen gas mixture preconditioning using vitro fertilization (IVFG) direct current electrical (ES20), to enhance the anti-inflammatory properties of sEVs canine adipose-derived MSCs (cAD-MSCs). Initial steps involved isolation comprehensive characterization cAD-MSCs, including morphology, gene expression, differentiation potentials, alongside validation protocol. IVFG, ES20, IVES were applied simultaneously with a control condition. Stimulated cAD-MSCs evaluated morphological changes, cell viability, expressions. Conditioned media collected purified sEV on Day1, Day2, Day3. To validate efficacy production, various analyses conducted, microscopic examination, surface marker assessment, zeta-potential measurement, protein quantification, nanoparticle tracking analysis, determination activity. We found that demonstrated non-cytotoxicity induced crucial genotypic changes associated production cAD-MSCs. Interestingly, IVFG influenced cellular adaptation, while ES20 hypoxia activation. By merging these stimulations, enhanced stability quality profiles. The cAD-MSC-derived exhibited activity lipopolysaccharide-induced RAW264.7 macrophages, emphasizing improved effectiveness without cytotoxicity or immunogenicity. These effects consistent across day 3 collection, indicating establishment effective protocol production. research established method positive impact characteristics stability, quality, not only contributes enhancement but also sheds light functional aspects interventions.
Language: Английский
Citations
0
Chem & Bio Engineering, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 18, 2025
Injectable hydrogels (IHs) have garnered significant attention in biomedical applications due to their minimally invasive nature, adaptability, and high degree of customization. However, traditional design methods IHs limitations addressing complex clinical needs, such as precise regulation the gelation time mechanical strength within a wide window. Hyperbranched polymers (HBPs), unique highly branched structures abundant functional sites, can be easily prepared functionalized enable decoupled modulation properties address challenges IHs. Our research group developed library HBPs via dynamically controllable polymerization method built series adjustable, controllable, responsive based on resulting HBPs. The fed by demonstrate an adjustable process, wide-range tuning properties, responsiveness demand, which show capabilities various applications. In this review, we summarize role self-healing ability, achieving through extending them broad range are still its infancy. This review provides overview fabricated variety multifunctional HBPs, diverse fields also presented. Meanwhile, point out future development potential challenges.
Language: Английский
Citations
0