Myosin Light Chains in the Progression of Cancer DOI Creative Commons

Savannah L. Kozole,

Karen A. Beningo

Cells, Journal Year: 2024, Volume and Issue: 13(24), P. 2081 - 2081

Published: Dec. 17, 2024

The myosin light chains (MLCs) of non-muscle II are known to regulate cellular architecture and generate forces; they also have an increasingly emerging role in the progression cancer. phosphorylation state controls activity myosins that implicated invasion proliferation. In cancers, when proliferation is greatly increased, cytokinesis relies on phosphorylated activate contractile forces used separate cells. Likewise, during metastasis, kinase pathways culminate aligning MLC structures for enhanced cell motility through stress fiber contraction accumulation filaments at leading edge. This review summarizes chain family members promote cancer evidence how their altered activities change behavior cells involving mechanical-based processes movements metastasis. addition, impact immune response cancers currently serve as biomarkers staging this disease; a brief summary these topics provided end review.

Language: Английский

Growing Role of 3D In Vitro Cell Cultures in the Study of Cellular and Molecular Mechanisms: Short Focus on Breast Cancer, Endometriosis, Liver and Infectious Diseases DOI Creative Commons
Nora Bloise, Marialaura Giannaccari, G Guagliano

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(12), P. 1054 - 1054

Published: June 18, 2024

Over the past decade, development of three-dimensional (3D) models has increased exponentially, facilitating unravelling fundamental and essential cellular mechanisms by which cells communicate with each other, assemble into tissues organs respond to biochemical biophysical stimuli under both physiological pathological conditions. This section presents a concise overview most recent updates on significant contribution different types 3D cell cultures including spheroids, organoids organ-on-chip bio-printed in advancing our understanding molecular mechanisms. The case studies presented include breast cancer (BC), endometriosis, liver microenvironment infections. In BC, establishment culture permitted visualization role cancer-associated fibroblasts delivery exosomes, as well significance physical properties extracellular matrix promoting proliferation invasion. approach also become valuable tool gaining insight general specific drug resistance. Given considerable heterogeneity offer more accurate representation vivo microenvironment, thereby identification translation novel targeted therapeutic strategies. advantages provided hepatic environment, conjunction high throughput characterizing various platforms, have enabled elucidation complex underlying threatening diseases. A limited number for gut skin infections been developed. However, profound comprehension spatial temporal interactions between microbes, host their environment may facilitate advancement vitro, ex disease models. Additionally, it pave way approaches diverse research fields. interested reader will find concluding remarks challenges prospects using discovering areas covered this review.

Language: Английский

Citations

7
Photochemistry as a tool for dynamic modulation of hydrogel mechanics DOI Creative Commons
Gretel Major, Habib Joukhdar, Yu Suk Choi

et al.

Cell Reports Physical Science, Journal Year: 2025, Volume and Issue: unknown, P. 102366 - 102366

Published: Jan. 1, 2025

SummaryPhotochemistry has emerged as a powerful tool for manipulating the dynamic and heterogeneous properties of hydrogel microenvironments in tissue engineering mechanobiology. Enhanced spatiotemporal control over mechanical can be achieved by incorporating an array photosensitive functional groups within polymer networks controlling photokinetics through light illumination. This review explores how light-stimulated photocleavage, addition, exchange, isomerization reactions are utilized to generate hydrogels that soften stiffen situ, enabling precise cell functionality tissue-engineered constructs. Advancements design biofabrication platforms have enhanced these permit local modulation larger discussed. The applications understanding cellular mechanosensation, investigating fibrotic disease, directing stem differentiation formation examined. While significant progress been made toward on-demand switch between multiple conditions, this highlights need materials undergo progressive stiffening. also emerging photochemistry intracellular environments its potential integration with advanced force spectroscopy techniques live-cell mechanobiology studies. Overall, using stimulus tunability, reaction kinetics, compared other stimuli-driven systems, opening new avenues biomimetic material mechanobiological investigations.Graphical abstract

Language: Английский

Citations

0
Interplay between extracellular matrix mechanics and cell function in mechanobiology DOI Creative Commons
Peter A. Galie, Paul A. Janmey

Current Opinion in Biomedical Engineering, Journal Year: 2025, Volume and Issue: unknown, P. 100589 - 100589

Published: March 1, 2025

Language: Английский

Citations

0
Silk fibroin: An innovative protein macromolecule-based hydrogel/scaffold revolutionizing breast cancer treatment and diagnosis – Mechanisms, advancements, and targeting capabilities DOI
Shubhrajit Mantry,

Kotaiah Silakabattini,

Prabhat Kumar Das

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142870 - 142870

Published: April 1, 2025

Language: Английский

Citations

0
Biomechanical Properties and Cellular Responses in Pulmonary Fibrosis DOI Creative Commons
Andong He, Lizhe He,

T.‐L. Chen

et al.

Bioengineering, Journal Year: 2024, Volume and Issue: 11(8), P. 747 - 747

Published: July 24, 2024

Pulmonary fibrosis is a fatal lung disease affecting approximately 5 million people worldwide, with 5-year survival rate of less than 50%. Currently, the only available treatments are palliative care and transplantation, as there no curative drug for this condition. The involves excessive synthesis extracellular matrix (ECM) due to alveolar epithelial cell damage, leading scarring stiffening tissue ultimately causing respiratory failure. Although multiple factors contribute disease, exact causes remain unclear. mechanical properties tissue, including elasticity, viscoelasticity, surface tension, not affected by but also its progression. This paper reviews alteration in these pulmonary progresses how cells lung, cells, fibroblasts, macrophages, respond changes, contributing exacerbation. Furthermore, it highlights importance developing advanced vitro models, based on hydrogels 3D bioprinting, which can accurately replicate structural fibrotic lungs conducive studying effects stimuli cellular responses. review aims summarize current understanding interaction between progression alterations properties, could aid development novel therapeutic strategies disease.

Language: Английский

Citations

3
Programmable materials: Current trends, challenges, and perspectives DOI Creative Commons
Giulia Scalet

Applied Materials Today, Journal Year: 2024, Volume and Issue: 40, P. 102372 - 102372

Published: Aug. 10, 2024

Research into programmable materials has attracted extraordinary interest since the nineties, when term "programmable" was introduced for first time. In its widest definition, is used to denote that are designed be highly dynamic, either in shape and/or physical/functional properties, on-demand and a precise, sequential predetermined way. Such unique feature allows them adapt various needs offers new opportunities several application fields, enabling overcome limitations of traditional materials. The present paper aims introduce readers world materials, enhance their interest, knowledge, skills field, provide useful insights ideas on how approach development implementation. Accordingly, this an overview discussion current state-of-the-art recent progress up future perspectives. First, historical evolution definition these as well types properties achievable presented. Then, different programming strategies could tune material covered, with emphasis constituent applied stimuli, geometrical arrangements. Finally, real-world applications, ongoing challenges, directions exciting class discussed.

Language: Английский

Citations

3
Recent advancements and challenges in 3D bioprinting for cancer applications DOI
Swayam Aryam Behera, Binita Nanda, P. Ganga Raju Achary

et al.

Bioprinting, Journal Year: 2024, Volume and Issue: 43, P. e00357 - e00357

Published: Sept. 13, 2024

Language: Английский

Citations

1
Challenges in Application: Gelation Strategies of DAT-Based Hydrogel Scaffolds DOI
Qiaoyu Li, Wei Liang, Huiting Wu

et al.

Tissue Engineering Part B Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: April 26, 2024

Decellularized Adipose Tissue (DAT) has great clinical applicability, owing to its abundant source material, natural extracellular matrix (ECM) microenvironment, and non-immunogenic attributes, rendering it a versatile resource in the realm of tissue engineering. However, practical implementations are confronted with multifarious limitations. Among these, selection an appropriate gelation strategy serves as foundation for adapting diverse contexts. The crosslinking strategies under varying physical or chemical conditions exert profound influences on ultimate morphology therapeutic efficacy DAT. This review sums up processes DAT decellularization subsequent gelation, specific emphasis employed recent experimental applications expounds upon methodologies, underlying principles, implications different strategies, aiming offer insights inspiration application engineering advance research scaffold development.

Language: Английский

Citations

0
Decellularized extracellular matrix-based bioengineered 3D breast cancer scaffolds for personalized therapy and drug screening DOI

Teeshyo Bhattacharya,

Mamta Kumari, Kulwinder Kaur

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(36), P. 8843 - 8867

Published: Jan. 1, 2024

Bioengineered 3D breast cancer model using responsive decellularized scaffold.

Language: Английский

Citations

0
Myosin Light Chains in the Progression of Cancer DOI Creative Commons

Savannah L. Kozole,

Karen A. Beningo

Cells, Journal Year: 2024, Volume and Issue: 13(24), P. 2081 - 2081

Published: Dec. 17, 2024

The myosin light chains (MLCs) of non-muscle II are known to regulate cellular architecture and generate forces; they also have an increasingly emerging role in the progression cancer. phosphorylation state controls activity myosins that implicated invasion proliferation. In cancers, when proliferation is greatly increased, cytokinesis relies on phosphorylated activate contractile forces used separate cells. Likewise, during metastasis, kinase pathways culminate aligning MLC structures for enhanced cell motility through stress fiber contraction accumulation filaments at leading edge. This review summarizes chain family members promote cancer evidence how their altered activities change behavior cells involving mechanical-based processes movements metastasis. addition, impact immune response cancers currently serve as biomarkers staging this disease; a brief summary these topics provided end review.

Language: Английский

Citations

0