Model-driven exploration of poro-viscoelasticity in human brain tissue: be careful with the parameters!

Interface Focus, Journal Year: 2024, Volume and Issue: 14(6)

Published: Dec. 6, 2024

The brain is arguably the most complex human organ and modelling its mechanical behaviour has challenged researchers for decades. There still a lack of understanding on how this multiphase tissue responds to loading material parameters can be reliably calibrated. While previous viscoelastic models with two relaxation times have successfully captured response tissue, Theory Porous Media provides continuum framework explore underlying physical mechanisms, including interactions between solid matrix free-flowing interstitial fluid. Following our previously published experimental testing protocol, here we perform finite element simulations cyclic compression–tension compression–relaxation experiments white gray matter specimens. volumetric stress proves crucial factor overall biphasic as it strongly interferes porous effects controlled by permeability. An inverse parameter identification reveals that poroelasticity alone insufficient capture time-dependent behaviour, but poro-viscoelastic formulation captures well. We provide valuable insights into individual contributions viscous effects. However, due strong coupling porous, viscous, effects, additional are required determine all parameters.

Language: Английский

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Test and tune: evaluating, adjusting and optimising the stiffness of hydrogels to influence cell fate

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 159295 - 159295

Published: Jan. 1, 2025

Language: Английский

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Comparative lipid profiling reveals the differential response of distinct lipid subclasses in blast and blunt-induced mild traumatic brain injury

Experimental Neurology, Journal Year: 2025, Volume and Issue: 385, P. 115141 - 115141

Published: Jan. 7, 2025

Language: Английский

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In Situ Sprayed Self‐Gelling Powder Self‐Assembled by a Pure Molecular Drug from Herbal Extract for Rapid Hemostasis and Neuroprotection in Traumatic Brain Injury

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 19, 2025

Abstract Immediate hemostasis and effective treatment are foremost for acute intervention in traumatic brain injury (TBI), but the therapeutic tools professional methods often lacking at scene. Self‐gelling powders formed by self‐assembly of pure molecular drugs can quickly deliver hemostatic effects without complications from chemical modifications or additional carriers. However, no clinical have been reported as a self‐gelling powder this purpose. Here, is self‐assembled sodium aescinate (SA), drug already approved treating trauma. To generate SA (SA self‐gel‐P), heated cooled an alkaline solution to form hydrogel, then freeze‐dried ground. The obtained self‐gel‐P has good hydrophilicity. When sprayed onto bleeding site after TBI, it rapidly absorbed blood gel through intermolecular hydrogen bonding, facilitating situ sealing rapid hemostasis. Furthermore, hydrogel released over long period, which helped reduce neuronal death, improve integrity blood‐brain barrier, edema, alleviate neuroinflammation, ultimately promote recovery neurofunction significant toxic side effects. This may serve powerful auxiliary tool pre‐hospital TBI.

Language: Английский

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Bridging biomechanics with neuropathological and neuroimaging insights for mTBI understanding through multiscale and multiphysics computational modeling

Biomechanics and Modeling in Mechanobiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

Language: Английский

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Title Actuated Hydrogel Platforms To Study Brain Cell Behavior

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 16, 2025

Abstract The human brain is a highly complex organ characterized by intricate neural networks, biochemical signaling, and unique mechanical properties. soft dynamic viscoelastic extracellular matrix (ECM) plays crucial role in supporting different types of cells influencing their behavior. Understanding how respond to stimuli within this environment essential for unraveling fundamental mechanisms healthy, unhealthy, regenerative functions the central nervous system. This requires development advanced materials techniques study interplay between cues cell responses. Hydrogels have become research, mimicking brain's ECM both chemical composition Conventional hydrogels, while helpful, are static lack stimulation. On other hand, hydrogels provide reversible, stimulation, closely replicating review discusses current hydrogel platforms used investigate function health disease, focusing on traumatic injury (TBI)‐like conditions tumors. These offer sophisticated tools understanding mechanobiology developing new therapeutic approaches.

Language: Английский

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Computational Modelling of Protected and Unprotected Head Impacts in Rugby

Bioengineering, Journal Year: 2025, Volume and Issue: 12(4), P. 361 - 361

Published: March 31, 2025

This study involved the simulation of five real-world head impact events in rugby, to assess level protection provided by a novel foam headguard, N-Pro. The University College Dublin Brain Trauma Model (UCDBTM) was used estimate peak resultant accelerations and brain tissue responses different scenarios. input kinematics were obtained from two sources: video analysis events, real-time data through instrumented mouthguards. simulated under both unprotected protected conditions. All simulations performed against rigid, non-compliant surface model. results this demonstrate significant potential N-Pro reducing stress/strain up c. 70% compared impacts. highlights headguard’s promising reduce severity impact-related injuries effectively attenuating stresses strains, as well linear rotational kinematics. Additionally, supports recommendation literature that kinematic collected wearable sensors should be supplemented improve accident reconstructions.

Language: Английский

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Modeling biomarker kinetics of Aβ levels in serum following blast

Frontiers in Neurology, Journal Year: 2025, Volume and Issue: 16

Published: April 4, 2025

Elucidating the unique neuropathological response to blast exposure remains a barrier towards development of diagnostic approaches for those with blast-induced traumatic brain injury (bTBI). Quantification biomarker concentrations in blood post-injury is typically used inform severity. However, progression and associated changes are sensitive parameters such as overpressure (BOP) magnitude frequency exposure. Through this work, blast-dose kinetics (BxK) platform was developed validated Aβ42 promising predictor post-blast. Blast-dose responses accounting BOP were integrated into mathematical model whole-body Aβ peptide kinetics. Validation performed through comparison acute monomer levels serum 15 service members exposed repeated low-level while undergoing three-day weapons training. Amyloid precursor protein (APP) synthesis assumed be proportional additive effects within window recovery applied account cumulative predicted 6.5 ± 5.2% on average, demonstrating feasibility sensitivity blast. Outcomes discuss how modulation patient-specific factors (age, weight, genetic factors, years exposure, sleep) pathophysiological (BBB permeability, amyloidogenic pathology, neuroinflammation) can reveal potential sources variability experimental data incorporated BxK future iterations. Advancements complexity sex-specific weapon system, stress levels, risk symptom onset, pharmacological treatment strategies anticipated improve calibration. Utilization identify drivers mechanisms predict chronic outcomes has transform bTBI diagnostic, prognostic, therapeutic strategies.

Language: Английский

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Mechanotransduction: Exploring New Therapeutic Avenues in Central Nervous System Pathology

Frontiers in Neuroscience, Journal Year: 2022, Volume and Issue: 16

Published: April 28, 2022

Cells are continuously exposed to physical forces and the central nervous system (CNS) is no exception. dynamically adapt their behavior remodel surrounding environment in response forces. The importance of mechanotransduction CNS illustrated by exploring its role pathology development progression. crosstalk between biochemical biophysical components extracellular matrix (ECM) here described, considering recent explosion literature demonstrating powerful influence stimuli like density, rigidity geometry ECM on cell behavior. This review aims at integrating mechanical properties into our understanding molecular basis disease. mechanisms that mediate events, integrin, Rho/ROCK metalloproteinases signaling pathways revised. Analysis pathologies this context has revealed a wide range neurological diseases share as hallmarks alterations tissue properties. Therefore, it belief may lead improved medical devices diagnostic methods well new therapeutic targets strategies for repair.

Language: Английский

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Functional Characterization of Mechanosensitive Piezo1 Channels in Trigeminal and Somatic Nerves in a Neuron-on-Chip Model

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(3), P. 1370 - 1370

Published: Jan. 25, 2022

Mechanosensitive ion channels, Piezo1 and 2, are activated by pressure involved in diverse physiological functions, including senses of touch pain, proprioception many more. Understanding their function is important for elucidating the mechanosensitive mechanisms a range human diseases. Recently, Piezo channels were suggested to be contributors migraine pain generation. Migraine typically characterized allodynia mechanical hyperalgesia associated with activation sensitization trigeminal ganglion (TG) nerve fibers. Notably, specific medicines ineffective other types suggesting distinct underlying mechanism. To address, straightforward manner, specificity mechanosensitivity vs. somatic nerves, we compared activity mouse TG neurons dorsal root ganglia (DRG) neurons. We assessed functional expression receptors using conventional live calcium imaging setup equipped multibarrel application system utilizing microfluidic chip-based setup. Surprisingly, neurons, despite higher gene, less responsive agonist Yoda1 than DRG This difference was more prominent setup, that certain limitations approach, such as turbulence, can overcome devices laminar solution flow.

Language: Английский

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