MicroRNAs Dependent G‐ELNs Based Intervention Improves Glucose and Fatty Acid Metabolism While Protecting Pancreatic β‐Cells in Type 2 Diabetic Mice DOI
Geetika Bajaj,

Diksha Choudhary,

Vishal Singh

et al.

Small, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 8, 2024

Abstract Metabolic disorders such as Type 2 diabetes mellitus (T2DM) imposes a significant global health burden. Plant‐derived exosome like nanoparticles (P‐ELNs) have emerged promising therapeutic alternate for various diseases. Present data demonstrates that treatment with Ginger‐derived (G‐ELNs) enhance insulin dependent glucose uptake, downregulate gluconeogenesis and oxidative stress in resistant HepG2 cells. Furthermore, oral administration of G‐ELNs T2DM mice decreases fasting blood levels improves tolerance effectively metformin. These improvements are attributed to the enhanced phosphorylation Protein kinase B (Akt‐2), phosphatidylinositol 3‐kinase at serine 474 which consequently leads increase hepatic sensitivity, improvement homeostasis decrease ectopic fat deposition. Oral also exerts protective effect on Streptozotocin (STZ)‐induced pancreatic β‐cells damage, contributing systemic amelioration T2DM. Further, per computational tools, miRNAs present modulate (PI3K)/Akt‐2 pathway exhibit strong interactions target mRNAs responsible gluconeogenesis, deposition stress. synthetic mimic miRNA downregulates its mRNA Overall, results indicate metabolism thus, exerting effects

Language: Английский

The microRNA Let-7 and its exosomal form: Epigenetic regulators of gynecological cancers DOI Creative Commons
Fei Wang,

Chundi Zhou,

Yanping Zhu

et al.

Cell Biology and Toxicology, Journal Year: 2024, Volume and Issue: 40(1)

Published: June 5, 2024

Abstract Many types of gynecological cancer (GC) are often silent until they reach an advanced stage, and therefore diagnosed too late for effective treatment. Hence, there is a real need more efficient diagnosis treatment patients with GC. During recent years, researchers have increasingly studied the impact microRNAs development, leading to number applications in detection MicroRNAs particular group tiny RNA molecules that regulate regular gene expression by affecting translation process. The downregulation numerous miRNAs has been observed human malignancies. Let-7 example miRNA controls cellular processes as well signaling cascades affect post-transcriptional expression. Recent research supports hypothesis enhancing let-7 those cancers where it downregulated may be potential option. Exosomes vesicles move through body fluids can include components like (including let-7) important communication between cells. Studies proved exosomes able enhance tumor growth, angiogenesis, chemoresistance, metastasis, immune evasion, thus suggesting their importance GC management. Graphical Impact on female malignancies diseases reproductive tract. dysregulated variety gynaecological obstetric disorders.

Language: Английский

Citations

5
Evaluation of circulating plasma miR-9, miR-29a, miR-192, and miR-375 as potential biomarkers for predicting prediabetes and type 2 diabetes in Nepali adult population DOI Creative Commons
Daya Ram Pokharel, Abhishek Maskey, Ramchandra Kafle

et al.

Non-coding RNA Research, Journal Year: 2024, Volume and Issue: 9(4), P. 1324 - 1332

Published: July 10, 2024

Language: Английский

Citations

4
microRNAs in Type 1 Diabetes: Roles, Pathological Mechanisms, and Therapeutic Potential DOI Open Access
H. Cho, Se Eun Ha, Rajan Singh

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3301 - 3301

Published: April 2, 2025

Type 1 diabetes (T1D) is a chronic autoimmune disease characterized by the progressive destruction of pancreatic β-cells, leading to insulin deficiency. The primary drivers β-cell in T1D involve autoimmune-mediated processes that trigger inflammation and ultimately loss. Regulatory microRNAs (miRNAs) play crucial role modulating these regulating gene expression through post-transcriptional suppression target mRNAs. Dysregulated miRNAs have been implicated pathogenesis, serving as both potential diagnostic biomarkers therapeutic targets. This review explores T1D, highlighting their involvement mechanisms across rodent models human patients. While current antidiabetic therapies manage symptoms, they do not prevent destruction, leaving patients reliant on lifelong therapy. By summarizing key miRNA profiles diabetic animal patients, this miRNA-based restore function halt or slow progression disease.

Language: Английский

Citations

0
RNA-based diagnostic innovations: A new frontier in diabetes diagnosis and management DOI Creative Commons
Esther Ugo Alum,

Ernest Nnamdi Ikpozu,

Christian Emeka Offor

et al.

Diabetes and Vascular Disease Research, Journal Year: 2025, Volume and Issue: 22(2)

Published: March 1, 2025

Background/Objective: Diabetes mellitus (DM) remains a major global health challenge due to its chronic nature and associated complications. Traditional diagnostic approaches, though effective, often lack the sensitivity required for early-stage detection. Recent advancements in molecular biology have identified RNA molecules, particularly non-coding RNAs such as microRNAs (miRNAs), long (lncRNAs), circular (circRNAs), promising biomarkers diabetes. This review aims explore role of RNA-based diagnosis, prognosis, management diabetes, highlighting their potential revolutionize diabetes care. Method: A comprehensive literature was conducted using electronic databases including PubMed, Scopus, Web Science. Articles published up 2024 were screened analyzed extract relevant findings related diagnostics Emphasis placed on studies demonstrating clinical utility, mechanistic insights, translational molecules. Results: Numerous species, miRNAs miR-375, miR-29, lncRNAs like H19 MEG3, exhibit altered expression patterns diabetic patients. These molecules are involved key regulatory pathways glucose metabolism, insulin resistance, β-cell function. Circulating detectable various biofluids, enabling non-invasive approaches. Emerging technologies, sequencing liquid biopsy platforms, enhanced specificity detection, fostering development novel tools personalized therapeutic strategies. Conclusion: hold significant promise advancing early risk stratification, monitoring Despite current challenges standardization validation, integration into routine practice could transform management, paving way precision medicine Further research multi-center trials essential validate these facilitate approval implementation.

Language: Английский

Citations

0
Long non-coding RNA LOXL1-AS1: a potential biomarker and therapeutic target in human malignant tumors DOI Creative Commons

Xiaoping Fu,

Chunyan Ji,

Wenqian Tang

et al.

Clinical and Experimental Medicine, Journal Year: 2024, Volume and Issue: 24(1)

Published: May 2, 2024

Abstract Long non-coding RNAs (lncRNAs) are transcripts that contain more than 200 nucleotides. Despite their inability to code proteins, multiple studies have identified important role in human cancer through different mechanisms. LncRNA lysyl oxidase like 1 antisense RNA (LOXL1-AS1), a newly discovered lncRNA located on chromosome 15q24.1, has recently been shown be involved the occurrence and progression of various malignancies, such as colorectal cancer, gastric hepatocellular carcinoma, prostate non-small cell lung ovarian cervical breast glioma, thymic pancreatic carcinoma. LOXL1-AS1 acts competitive endogenous (ceRNA) via sponging miRNAs, including miR-374b-5p, miR-21, miR-423-5p, miR-589-5p, miR-28-5p, miR-324-3p, miR-708-5p, miR-143-3p, miR-18b-5p, miR-761, miR-525-5p, miR-541-3p, miR-let-7a-5p, miR-3128, miR-3614-5p, miR-377-3p miR-1224-5p promote tumor proliferation, invasion, migration, apoptosis, cycle, epithelial–mesenchymal transformation (EMT). In addition, is regulation P13K/AKT MAPK signaling pathways. This article reviews current understanding biological function clinical significance cancers. These findings suggest may both reliable biomarker potential therapeutic target for

Language: Английский

Citations

3
Mechanistic and therapeutic perspectives of miRNA-PTEN signaling axis in cancer therapy resistance DOI
Di Wu, Chunjie Huang,

Kaifeng Guan

et al.

Biochemical Pharmacology, Journal Year: 2024, Volume and Issue: 226, P. 116406 - 116406

Published: July 4, 2024

Language: Английский

Citations

3
Gut microbiota–lncRNA/circRNA crosstalk: implications for different diseases DOI
Lei Zhang, Xin Li,

Huijuan Gao

et al.

Critical Reviews in Microbiology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 15

Published: July 5, 2024

The gut microbiota features an abundance of diverse microorganisms and represents important component human physiology metabolic homeostasis, indicating their roles in a wide array physiological pathological processes the host. Maintaining balance is critical for normal functionality as microbial dysbiosis can lead to occurrence development diseases through various mechanisms. Long non-coding RNAs (lncRNAs) circular (circRNAs) are that perform regulatory functions many processes. Furthermore, lncRNAs/circRNAs known interact range both activities. In this article, we review existing research relevant interaction between investigate role crosstalk pathogenesis different diseases. Studies have shown that, target lncRNAs ENO1-IT1, BFAL1, LINC00152 regulate colorectal cancer

Language: Английский

Citations

2
The scaffold protein disabled 2 (DAB2) and its role in tumor development and progression DOI
Disha V. Pandya, Rajsi V. Parikh, Ruhanahmed M. Gena

et al.

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: June 1, 2024

Language: Английский

Citations

1
G protein-coupled receptor 1 participating in the mechanism of mediating gestational diabetes mellitus by phosphorylating the AKT pathway DOI Creative Commons
Yanbin Zhu,

Shufeng Huang,

Dan Chai

et al.

Open Life Sciences, Journal Year: 2024, Volume and Issue: 19(1)

Published: Jan. 1, 2024

Abstract Gestational diabetes mellitus (GDM) is a metabolic disease that occurs during pregnancy. Herein, we investigate G protein-coupled receptor 1 (GPR1) in mediating GDM through the phosphorylation of serine/threonine kinase (AKT) pathway. Thirty pregnant SD rats were grouped into: normal pregnancy control group (NC), model group, and + high-dose GPR1 antagonist treatment (GDM Ari) group. was established, Ari adopted aripiprazole. The blood glucose level, insulin resistance (IR) detected. expression GPR1, AKT, extracellular signal-regulated (ERK) placental tissue detected using reverse transcription-polymerase chain reaction (RT-PCR) western blotting (WB). serum concentration, glycated hemoglobin concentration significantly higher than those NC ( P < 0.05). levels ERK lower Furthermore, compared with reduced while simultaneously enhancing level IR level. In addition, survival number, body weight, malformation rate offspring improved, there no significant effect on number offspring. expressions exhibited decrease, observed to increase Enhancing may activate AKT alleviate GDM. could potentially serve as novel target for treatment, offering new insights into managing

Language: Английский

Citations

0
MCM4 Promotes the Progression of Malignant Melanoma by Activating the PI3K/AKT Pathway DOI
Xuewei Zhang,

Mingming Dong,

Guoxing Zheng

et al.

Environmental Toxicology, Journal Year: 2024, Volume and Issue: 40(2), P. 306 - 317

Published: Nov. 6, 2024

ABSTRACT This study aims to elucidate the role of minichromosome maintenance protein 4 (MCM4) in malignant melanoma (MM) and explore underlying mechanism. Initially, data from The Cancer Genome Atlas (TCGA) database Molecular Signature Database (MSigDB) were used investigate biological impact MCM4 on MM. Further, a prognostic model using Cox regression analysis was developed predict overall survival (OS) rate MM patients. effects proliferation, migration, invasion abilities (B16F0 A375) cells demonstrated CCK‐8, colony formation, EDU, wound scratch, Transwell assays. In subcutaneous tumor models C57BL/6 mice vivo, expression levels tumors detected Western blot immunofluorescence approaches. bioinformatics indicated that expressed higher tissues than normal ( p < 0.05). established OS prediction could significantly contribute devising follow‐up strategies treating knockdown resulted reduced cells, which reversed by overexpression Moreover, activate phosphatidylinositol 3′‐kinase (PI3K)/AKT pathway cells. PI3K inhibitor (LY294002) reverse substantially prompt growth through PI3K/AKT vivo. summary, prompted development metastasis activating pathway.

Language: Английский

Citations

0