Silencing of maternally expressed RNAs in Dlk1-Dio3 domain causes fatal vascular injury in the fetal liver DOI Creative Commons

Haoran Yu,

Yue Zhao, Rui Cheng

et al.

Cellular and Molecular Life Sciences, Journal Year: 2024, Volume and Issue: 81(1)

Published: Oct. 9, 2024

The mammalian imprinted Dlk1-Dio3 domain contains multiple lncRNAs, mRNAs, the largest miRNA cluster in genome and four differentially methylated regions (DMRs), deletion of maternally expressed RNA within this locus results embryonic lethality, but mechanism by which occurs is not clear. Here, we optimized model RNAs transcription termination found that cause death was apoptosis embryo, particularly liver. We generated a mouse silencing inserting 3 × polyA sequence into Gtl2 locus. By analyzing RNA-seq data embryos combined with histological analysis, activated apoptosis, causing vascular rupture fetal liver, resulting hemorrhage injury. Mechanistically, activation paternally genes interval, it gene itself rather than IG-DMR Gtl2-DMR causes aforementioned phenotypes. In conclusion, these findings illuminate novel leads to hepatic through apoptosis.

Language: Английский

Integrated bioinformatics and experimental analysis of CHAF1B as a novel biomarker and immunotherapy target in LUAD DOI Creative Commons
Wei Du, Xiaowei Wu, Qing‐Feng Li

et al.

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 14, 2025

The prognosis and treatment efficacy of lung adenocarcinoma (LUAD), a disease with high incidence, remains unsatisfactory. Identifying new biomarkers therapeutic targets for LUAD is essential. Chromosomal assembly factor 1B (CHAF1B), p60 component the CAF-1 complex, closely linked to tumor incidence cell proliferation. However, CHAF1B's biological role molecular mechanism in remain unclear. Here, CHAF1B expression was examined using GEPIA2 UALCAN databases. Using Cancer Genome Atlas (TCGA) database, we analyzed diagnostic prognostic significance its association immune infiltration immunological checkpoints. Gene ontology (GO) enrichment single-cell function analyses were employed investigate possible roles. Drug sensitivity analysis predicted effect on chemotherapeutic drug sensitivity. We also lncRNAs-miRNA-CHAF1B axis explore LUAD. Preliminary vitro studies qRT-PCR, CCK8, Transwell, glucose, lactate metabolism confirmed Its associated sensitivity, checkpoints, infiltration. that three miRNAs (miR-29c-3p, miR-145-5p, miR-1247-5p) lncRNAs (AL139287.1, NEAT1, SHG1) may be target regulate CHAF1B. In tests showed suppression decreased LUAD's migration, invasion, proliferation, glycolysis. Overall, an innovative biomarker

Language: Английский

Citations

1
Autophagy-related long non-coding RNA MIR210HG plays a therapeutic role in hepatocellular carcinoma DOI Creative Commons
Chaoqun Zhang,

Dianxing Sun,

Huifang Zhou

et al.

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 21, 2025

This study aimed to investigate the role of autophagy-related long noncoding RNA (lncRNA) MIR210HG in hepatocellular carcinoma and its potential as a therapeutic target. LncRNA expression correlation with survival outcomes patients were analyzed using data from The Cancer Genome Atlas (TCGA). Kaplan–Meier Cox regression analyses conducted assess correlations. Quantitative reverse transcription PCR was used measure lncRNA liver cancer cells normal cells. Functional assays, including CCK-8, Transwell, flow cytometry, western blot, performed evaluate effects on cell proliferation, invasion, apoptosis, autophagy carcinoma. Elevated correlated poor overall patients. significantly up-regulated compared Knockdown inhibited proliferation autophagy, while promoting apoptosis cells, findings that confirmed through both vitro vivo studies. suggest contributes progression by regulating could serve promising target treatment strategies.

Language: Английский

Citations

1
Long Non-Coding RNAs in Humans: Classification, Genomic Organization and Function DOI Creative Commons

Barbara Chodurska,

Tanja Kunej

Non-coding RNA Research, Journal Year: 2025, Volume and Issue: 11, P. 313 - 327

Published: Jan. 14, 2025

Long non-coding RNAs (lncRNAs) regulate numerous biological functions in animals. Despite recent advances lncRNA research, their structural and functional annotation classification remain an ongoing challenge. This review provides a comprehensive overview of human lncRNAs, highlighting genomic organization, mode action role physiological pathological processes. Subgroups genes are discussed using representative examples visualizations organization. The HUGO Gene Nomenclature Committee (HGNC) categorizes lncRNAs into nine subgroups: (1) microRNA host genes, (2) small nucleolar RNA (3) long intergenic non-protein coding (LINC), (4) antisense RNAs, (5) overlapping transcripts, (6) intronic (7) divergent (8) with non-systematic symbols (9) FAM root systems. Circular (circRNAs) separate class that shares some characteristics divided exonic, intronic-exonic types. LncRNAs act as molecular signals, decoys, scaffolds sponges for microRNAs often function competing endogenous (ceRNAs). involved various processes, such cell differentiation, p53-mediated DNA damage response, glucose metabolism, inflammation immune functions. They associated several diseases, including types neoplasms, Alzheimer's disease autoimmune diseases. A clear system is essential understanding facilitating practical applications biomedical research. Future studies should focus on drug development biomarker discovery. As important regulators represent promising targets innovative therapies.

Language: Английский

Citations

0
OTUD6B-AS1: a multifaceted regulator of cancer with critical clinical implications DOI
Zihan Zheng,

Chenchen Zhou,

Yi Fan

et al.

American Journal of Cancer Research, Journal Year: 2025, Volume and Issue: 15(1), P. 1 - 18

Published: Jan. 1, 2025

OTU Deubiquitinase 6B-Antisense Transcript 1 (OTUD6B-AS1), a novel long non-coding RNA (lncRNA), has recently emerged as critical regulator in various tumors. Current research underscores its dual functionality, acting either an oncogene or tumor suppressor depending on the context. In this work, we compile and discuss findings from range of studies investigating expression patterns OTUD6B-AS1 different cancers consequent effects behavior, both vitro vivo. We delve into mechanisms through which influences cancer initiation progression, focusing role regulating essential cellular processes such cell growth, migration, invasion, angiogenesis, ferroptosis, treatment resistance. Operating complex interactions with microRNAs (miRNAs), proteins, pivotal signaling pathways - most notably Wnt/β-catenin exhibits variable roles across types environments. Additionally, assess clinical relevance levels, evaluating potential biomarker for prognosis diagnosis, well target therapeutic intervention. By consolidating existing knowledge, work aims to highlight implications encourage further oncology, ultimately contributing advancement targeted therapies.

Language: Английский

Citations

0
lncRNAs: the unexpected link between protein synthesis and cancer adaptation DOI Creative Commons
Mila Gugnoni, Manoj Kumar Kashyap, Kishore K. Wary

et al.

Molecular Cancer, Journal Year: 2025, Volume and Issue: 24(1)

Published: Jan. 31, 2025

Cancer progression relies on the ability of cells to adapt challenging environments overcoming stresses and growth constraints. Such adaptation is a multifactorial process that depends rapid reorganization many basic cellular mechanisms. Protein synthesis often dysregulated in cancer, translational reprogramming emerging as driving force cancer adaptive plasticity. Long non-coding RNAs (lncRNAs) represent main product genome transcription. They outnumber mRNAs by an order magnitude their expression regulated extremely specific manner depending context, space time. This heterogeneity functional allows lncRNAs act context-specific, fine-tuning controllers gene expression. Multiple recent evidence underlines how, besides consolidated role transcription, are major players translation control. Their capacity establish multiple highly dynamic interactions with proteins other transcripts makes these molecules able play central across all phases protein synthesis. Even if through myriad different mechanisms, action dual. On one hand, modulating overall speed, participate metabolic under stress conditions. prioritizing they help maintain high levels essential oncogenes. In this review, we aim discuss most relevant regarding involvement regulation how function may affect plasticity resistance stress. We also expect provide first collective perspectives way modulate beyond

Language: Английский

Citations

0
Role of non-coding RNAs in the pathogenesis of viral myocarditis DOI Creative Commons

Zhijuan Kang,

Liang Zhang, Zuocheng Yang

et al.

Virulence, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 14, 2025

Viral myocarditis (VMC) is a common inflammatory disease of the myocardium that characterized mainly by cell infiltration and cardiomyocyte necrosis. Coxsackievirus B3 (CVB3) cause VMC, although major progress has been made in treatment long-term prognosis still not ideal further research needed. Non-coding RNAs (ncRNAs) are RNA molecules without coding functions include microRNAs (miRNAs), long non-coding (lncRNAs) circular (circRNAs), which play extensive regulatory roles gene expression; however, their mechanisms action CVB3-induced VMC remain incompletely understood. Here, we review currently known various ncRNAs models, with focus on death, inflammation viral replication, aim providing reference for therapeutic or vaccine development VMC.

Language: Английский

Citations

0
LncRNA HOTTIP as a potential biomarker of chronic periodontitis and its role in inflammatory responses DOI
Jing Guo,

He Ying

Odontology, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Language: Английский

Citations

0
Insights into the Mode and Mechanism of Interactions Between RNA and RNA-Binding Proteins DOI Open Access
Yan Fang,

Xinyu Liu,

Yuli Liu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11337 - 11337

Published: Oct. 22, 2024

Both RNA and protein play important roles in the process of gene expression regulation, it has been widely discussed that interactions between affect transcription, translation efficiency, post-translational modification. As an class proteins, RNA-binding proteins bind to various ways. Here, we review structural functional properties illustrate specific modes describe involvement some representative families this network action. Furthermore, also explore association exists onset diseases, as well their potential terms serving a therapeutic tool for treatment diseases. The in-depth exploration reveals dynamic offering valuable insights advance progress dissection disease mechanisms research discovery drugs, which promote development molecular biology.

Language: Английский

Citations

1
Silencing of maternally expressed RNAs in Dlk1-Dio3 domain causes fatal vascular injury in the fetal liver DOI Creative Commons

Haoran Yu,

Yue Zhao, Rui Cheng

et al.

Cellular and Molecular Life Sciences, Journal Year: 2024, Volume and Issue: 81(1)

Published: Oct. 9, 2024

The mammalian imprinted Dlk1-Dio3 domain contains multiple lncRNAs, mRNAs, the largest miRNA cluster in genome and four differentially methylated regions (DMRs), deletion of maternally expressed RNA within this locus results embryonic lethality, but mechanism by which occurs is not clear. Here, we optimized model RNAs transcription termination found that cause death was apoptosis embryo, particularly liver. We generated a mouse silencing inserting 3 × polyA sequence into Gtl2 locus. By analyzing RNA-seq data embryos combined with histological analysis, activated apoptosis, causing vascular rupture fetal liver, resulting hemorrhage injury. Mechanistically, activation paternally genes interval, it gene itself rather than IG-DMR Gtl2-DMR causes aforementioned phenotypes. In conclusion, these findings illuminate novel leads to hepatic through apoptosis.

Language: Английский

Citations

0