Circadian Rhythms and Sleep in Drosophila melanogaster

Genetics, Journal Year: 2017, Volume and Issue: 205(4), P. 1373 - 1397

Published: March 30, 2017

The advantages of the model organism Drosophila melanogaster, including low genetic redundancy, functional simplicity, and ability to conduct large-scale screens, have been essential for understanding molecular nature circadian (∼24 hr) rhythms, continue be valuable in discovering novel regulators rhythms sleep. In this review, we discuss current these interrelated biological processes wider implications research. Clock genes period timeless were first discovered screens developed 1970s. Feedback on their own transcription forms core clock, accurately timed expression, localization, post-transcriptional modification, function is thought critical maintaining cycle. Regulators, several phosphatases kinases, act different steps feedback loop ensure strong rhythms. Approximately 150 neurons fly brain that contain components clock together translate intracellular cycling into rhythmic behavior. We how groups serve functions allowing clocks entrain environmental cues, driving behavioral outputs at times day, flexible responses conditions. neuropeptide PDF provides an important signal synchronize neurons, although details accomplishes are still being explored. Secreted signals from also influence other tissues. SLEEP is, part, regulated by which ensures appropriate timing sleep, but amount quality sleep determined mechanisms a homeostatic balance between wake. Flies useful identifying large set genes, molecules, neuroanatomic loci regulating amount. Conserved aspects regulation flies mammals include wake-promoting roles catecholamine neurotransmitters involvement hypothalamus-like regions, regions implicated less clear parallels. Sleep subject factors such as food availability, stress, social environment. beginning understand identified molecules interact with each other, environment, regulate researchers can take advantage increasing mechanistic behaviors, learning memory, courtship, aggression, loss impacts behaviors. thus remain tool both discovery deep

Language: Английский

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Sleep Drive Is Encoded by Neural Plastic Changes in a Dedicated Circuit

Cell, Journal Year: 2016, Volume and Issue: 165(6), P. 1347 - 1360

Published: May 21, 2016

Language: Английский

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A connectome of the Drosophila central complex reveals network motifs suitable for flexible navigation and context-dependent action selection

eLife, Journal Year: 2021, Volume and Issue: 10

Published: Oct. 26, 2021

Flexible behaviors over long timescales are thought to engage recurrent neural networks in deep brain regions, which experimentally challenging study. In insects, circuit dynamics a region called the central complex (CX) enable directed locomotion, sleep, and context- experience-dependent spatial navigation. We describe first complete electron microscopy-based connectome of

Language: Английский

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Neuroarchitecture and neuroanatomy of the Drosophila central complex: A GAL4‐based dissection of protocerebral bridge neurons and circuits

The Journal of Comparative Neurology, Journal Year: 2014, Volume and Issue: 523(7), P. 997 - 1037

Published: Nov. 7, 2014

ABSTRACT Insects exhibit an elaborate repertoire of behaviors in response to environmental stimuli. The central complex plays a key role combining various modalities sensory information with insect's internal state and past experience select appropriate responses. Progress has been made understanding the broad spectrum outputs from neuropils circuits involved numerous behaviors. Many resident neurons have also identified. However, specific roles these intricate structures functional connections between them remain largely obscure. Significant gains rely on obtaining comprehensive catalog associated GAL4 lines that arborize within brain regions, mapping neuronal pathways connecting structures. To this end, small populations Drosophila melanogaster were stochastically labeled using multicolor flip‐out technique was created neurons, their morphologies, trajectories, relative arrangements, corresponding lines. This report focuses one structure complex, protocerebral bridge, identifies just 17 morphologically distinct cell types structure. work provides new insights into anatomical four components its accessory neuropils. Most strikingly, we found bridge contains 18 glomeruli, not 16, as previously believed. Revised wiring diagrams take account updated architectural design are presented. map will facilitate deeper behavioral physiological dissection sophisticated set J. Comp. Neurol. 523:997–1037, 2015. © 2014 Wiley Periodicals, Inc.

Language: Английский

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Chemoconnectomics: Mapping Chemical Transmission in Drosophila

Neuron, Journal Year: 2019, Volume and Issue: 101(5), P. 876 - 893.e4

Published: Feb. 21, 2019

Language: Английский

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Operation of a homeostatic sleep switch

Nature, Journal Year: 2016, Volume and Issue: 536(7616), P. 333 - 337

Published: Aug. 2, 2016

Language: Английский

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Sleep Facilitates Memory by Blocking Dopamine Neuron-Mediated Forgetting

Cell, Journal Year: 2015, Volume and Issue: 161(7), P. 1656 - 1667

Published: June 1, 2015

Early studies from psychology suggest that sleep facilitates memory retention by stopping ongoing retroactive interference caused mental activity or external sensory stimuli. Neuroscience research with animal models, on the other hand, suggests enhancing consolidation. Recently, in Drosophila, of specific dopamine neurons was shown to regulate forgetting olfactory memories. Here, we show this dopaminergic is modulated behavioral state, increasing robustly locomotor and decreasing rest. Increasing sleep-drive, either sleep-promoting agent Gaboxadol genetic stimulation neural circuit for sleep, decreases activity, while retention. Conversely, arousal stimulates accelerates dopaminergic-based forgetting. Therefore, regulated state modulation plasticity. Our findings integrate psychological neuroscience

Language: Английский

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Neuronal Mechanisms for Sleep/Wake Regulation and Modulatory Drive

Neuropsychopharmacology, Journal Year: 2017, Volume and Issue: 43(5), P. 937 - 952

Published: Dec. 5, 2017

Language: Английский

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Unraveling the Evolutionary Determinants of Sleep

Current Biology, Journal Year: 2016, Volume and Issue: 26(20), P. R1073 - R1087

Published: Oct. 1, 2016

Language: Английский

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Recurrent Circuitry for Balancing Sleep Need and Sleep

Neuron, Journal Year: 2018, Volume and Issue: 97(2), P. 378 - 389.e4

Published: Jan. 1, 2018

Sleep-promoting neurons in the dorsal fan-shaped body (dFB) of Drosophila are integral to sleep homeostasis, but how these cells impose on organism is unknown. We report that dFB communicate via inhibitory transmitters, including allatostatin-A (AstA), with interneurons connecting superior arch ellipsoid central complex. These "helicon cells" express galanin receptor homolog AstA-R1, respond visual input, gate locomotion, and inhibited by AstA, suggesting promote rest suppressing visually guided movement. Sleep changes caused enhanced or diminished allatostatinergic transmission from inhibition optogenetic stimulation helicon support this notion. Helicon provide excitation R2 body, whose activity-dependent plasticity signals rising pressure dFB. By virtue autoregulatory loop, dFB-mediated interrupts processes incur a debt, allowing restorative rebalance books. VIDEO ABSTRACT.

Language: Английский

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