Molecular interrogation of hypothalamic organization reveals distinct dopamine neuronal subtypes

Nature Neuroscience, Journal Year: 2016, Volume and Issue: 20(2), P. 176 - 188

Published: Dec. 19, 2016

Language: Английский

---

Anatomically Defined and Functionally Distinct Dorsal Raphe Serotonin Sub-systems

Cell, Journal Year: 2018, Volume and Issue: 175(2), P. 472 - 487.e20

Published: Aug. 23, 2018

Language: Английский

---

Monosynaptic Circuit Tracing with Glycoprotein-Deleted Rabies Viruses

Journal of Neuroscience, Journal Year: 2015, Volume and Issue: 35(24), P. 8979 - 8985

Published: June 17, 2015

### Introduction Since the introduction of methods using glycoprotein (G)-deleted rabies virus (RV dG ) to identify direct monosynaptic inputs genetically targeted neurons eight years ago ([Wickersham et al., 2007b][1]), this approach has been widely used for sophisticated circuit-tracing

Language: Английский

---

Serotonin neurons in the dorsal raphe nucleus encode reward signals

Nature Communications, Journal Year: 2016, Volume and Issue: 7(1)

Published: Jan. 28, 2016

The dorsal raphe nucleus (DRN) is involved in organizing reward-related behaviours; however, it remains unclear how genetically defined neurons the DRN of a freely behaving animal respond to various natural rewards. Here we addressed this question using fibre photometry and single-unit recording from serotonin (5-HT) GABA mice. Rewards including sucrose, food, sex social interaction rapidly activate 5-HT neurons, but aversive stimuli quinine footshock do not. Both expected unexpected rewards neurons. After mice learn wait for sucrose delivery, most fire tonically during waiting then phasically on reward acquisition. Finally, are activated by inhibited when seek Thus, positively encode wide range signals anticipatory consummatory phases responses. Moreover, play complementary role processing.

Language: Английский

---

Serotonergic neurons signal reward and punishment on multiple timescales

eLife, Journal Year: 2015, Volume and Issue: 4

Published: Feb. 25, 2015

Serotonin's function in the brain is unclear. One challenge testing numerous hypotheses about serotonin's has been observing activity of identified serotonergic neurons animals engaged behavioral tasks. We recorded dorsal raphe while mice experienced a task which rewards and punishments varied across blocks trials. ‘tagged’ with light-sensitive protein channelrhodopsin-2 them based on their responses to light. found three main features neuron activity: (1) large fraction modulated tonic firing rates over course minutes during reward vs punishment blocks; (2) most were phasically excited by punishments; (3) subset was reward-predicting cues. By contrast, dopaminergic did not show rate changes These results suggest that signal information multiple timescales.

Language: Английский

---

Overlapping Brain Circuits for Homeostatic and Hedonic Feeding

Cell Metabolism, Journal Year: 2017, Volume and Issue: 27(1), P. 42 - 56

Published: Nov. 5, 2017

Language: Английский

---

A High-ResolutionIn VivoAtlas of the Human Brain's Serotonin System

Journal of Neuroscience, Journal Year: 2016, Volume and Issue: 37(1), P. 120 - 128

Published: Nov. 17, 2016

The serotonin (5-hydroxytryptamine, 5-HT) system modulates many important brain functions and is critically involved in neuropsychiatric disorders. Here, we present a high-resolution, multidimensional, vivo atlas of four the human brain's 5-HT receptors (5-HT 1A , 1B 2A 4 ) transporter (5-HTT). created from molecular structural high-resolution neuroimaging data consisting positron emission tomography (PET) magnetic resonance imaging (MRI) scans acquired total 210 healthy individuals. Comparison regional PET binding measures with postmortem autoradiography outcomes showed high correlation for five targets this enabled us to transform represent protein densities (in picomoles per milliliter). We also assessed association between concentration mRNA expression by comparing density across Allen Human Brain identified receptor- transporter-specific associations that show relation two measures. Together, these provide unparalleled insight into brain. SIGNIFICANCE STATEMENT (PET)- imaging-based transporter. PET-derived correlate strongly corresponding levels. strong enables transformation map system. Next, compared receptor/transporter levels uncovered unique at detail. This new not only provides synthesis, transport, density, but represents valuable source information neuroscience community as comparative instrument assess

Language: Английский

---

The ventral pallidum: Subregion-specific functional anatomy and roles in motivated behaviors

Progress in Neurobiology, Journal Year: 2015, Volume and Issue: 130, P. 29 - 70

Published: April 9, 2015

Language: Английский

---

Rabies Virus CVS-N2c ΔG Strain Enhances Retrograde Synaptic Transfer and Neuronal Viability

Neuron, Journal Year: 2016, Volume and Issue: 89(4), P. 711 - 724

Published: Jan. 21, 2016

Language: Английский

---

Dopamine neurons projecting to the posterior striatum form an anatomically distinct subclass

eLife, Journal Year: 2015, Volume and Issue: 4

Published: Aug. 31, 2015

Combining rabies-virus tracing, optical clearing (CLARITY), and whole-brain light-sheet imaging, we mapped the monosynaptic inputs to midbrain dopamine neurons projecting different targets (different parts of striatum, cortex, amygdala, etc) in mice. We found that most populations receive a similar set rather than forming strong reciprocal connections with their target areas. A common feature among was existence dense 'clusters' within ventral striatum. However, posterior striatum were outliers, receiving relatively few from instead more globus pallidus, subthalamic nucleus, zona incerta. These results lay foundation for understanding input/output structure circuit demonstrate constitute unique class regulated by inputs.

Language: Английский

---