Journal of Neuroscience,
Journal Year:
2020,
Volume and Issue:
40(12), P. 2485 - 2497
Published: Feb. 12, 2020
We
recently
developed
a
rat
model
of
relapse
to
drug
seeking
after
food
choice-induced
voluntary
abstinence.
Here,
we
used
this
study
the
role
orbitofrontal
cortex
(OFC)
and
its
afferent
projections
in
fentanyl
seeking.
trained
male
female
rats
self-administer
palatable
pellets
for
6
d
(6
h/d)
intravenous
(2.5
μg/kg/infusion)
12
h/d).
assessed
13–14
abstinence
days,
achieved
through
discrete
choice
procedure
between
infusions
(20
trials/d).
In
both
sexes,
was
associated
with
increased
expression
activity
marker
Fos
OFC.
Pharmacological
inactivation
OFC
muscimol
plus
baclofen
(50
+
50
ng/side)
decreased
then
determined
projection-specific
activation
afferents
during
test
by
using
retrograde
tracer
cholera
toxin
B
(injected
into
OFC).
Relapse
piriform
(Pir)
neurons
projecting
OFC,
but
not
from
basolateral
amygdala
thalamus.
Pir
Next,
an
anatomical
disconnection
determine
whether
are
critical
Unilateral
injections
one
hemisphere
unilateral
contralateral,
ipsilateral,
relapse.
Our
results
identify
Pir–OFC
as
new
motivation-related
pathway
opioid
SIGNIFICANCE
STATEMENT
There
few
preclinical
studies
relapse,
these
have
experimenter-imposed
extinction
or
forced
procedures.
humans,
however,
is
often
voluntary,
available
environment
forgone
favor
nondrug
alternative
reinforcers.
classical
pharmacology,
immunohistochemistry,
tracing
demonstrate
cortices
Nature Communications,
Journal Year:
2023,
Volume and Issue:
14(1)
Published: Aug. 21, 2023
Abstract
Responses
of
the
insular
cortex
(IC)
and
amygdala
to
stimuli
positive
negative
valence
are
altered
in
patients
with
anxiety
disorders.
However,
neural
coding
both
by
IC
neurons
remains
unknown.
Using
fiber
photometry
recordings
mice,
we
uncover
a
selective
increase
activity
projection
anterior
(aIC),
but
not
posterior
(pIC)
section,
when
animals
exploring
anxiogenic
spaces,
this
is
proportional
level
mice.
Neurons
aIC
also
respond
valence,
strength
response
strong
mice
levels
anxiety.
ex
vivo
electrophysiology,
characterized
connection
basolateral
(BLA),
employed
projection-specific
optogenetics
reveal
properties
aIC-BLA
neurons.
Finally,
identified
that
activated
as
well
aversive
stimuli,
activities
positively
correlated.
Altogether,
common
neurobiological
substrate
linking
anxiety-related
information
behaviors,
which
provides
starting
point
understand
how
alterations
these
populations
contribute
psychiatric
Journal of Neuroscience,
Journal Year:
2017,
Volume and Issue:
37(45), P. 10867 - 10876
Published: Nov. 8, 2017
High
rates
of
relapse
to
drug
use
during
abstinence
is
a
defining
feature
human
addiction.
This
clinical
scenario
has
been
studied
at
the
preclinical
level
using
different
animal
models
in
which
seeking
assessed
after
cessation
operant
self-administration
rodents
and
monkeys.
In
our
Society
for
Neuroscience
(SFN)
session
entitled
“Circuit
Synaptic
Plasticity
Mechanisms
Drug
Relapse,”
we
will
discuss
new
developments
understanding
circuits
synaptic
plasticity
mechanisms
from
studies
combining
established
novel
with
state-of-the-art
cellular,
electrophysiology,
anatomical,
chemogenetic,
optogenetic
methods.
We
also
translational
implications
these
developments.
mini-review
that
introduces
SFN
session,
summarize
results
laboratories
on
behavioral,
circuit
within
context
session.
British Journal of Pharmacology,
Journal Year:
2018,
Volume and Issue:
175(7), P. 994 - 1003
Published: Jan. 16, 2018
The
last
decade
has
seen
major
advances
in
neuroscience
tools
allowing
us
to
selectively
modulate
cellular
pathways
freely
moving
animals.
Chemogenetic
approaches
such
as
designer
receptors
exclusively
activated
by
drugs
(DREADDs)
permit
the
remote
control
of
neuronal
function
systemic
drug
administration.
These
have
dramatically
advanced
our
understanding
neural
behaviour.
Here,
we
review
different
techniques
and
genetic
available
for
restriction
chemogenetic
defined
populations.
We
highlight
use
a
dual
virus
approach
target
specific
circuitries
effectiveness
routes
administration
drugs.
Finally,
discuss
potential
caveats
associated
with
DREADDs
including
off-target
effects
drugs,
chronic
receptor
activation
issue
collateral
projections
DREADD
inhibition.
