Engineering of NEMO as calcium indicators with large dynamics and high sensitivity

Nature Methods, Journal Year: 2023, Volume and Issue: 20(6), P. 918 - 924

Published: April 20, 2023

Genetically encoded calcium indicators (GECIs) are indispensable tools for real-time monitoring of intracellular signals and cellular activities in living organisms. Current GECIs face the challenge suboptimal peak signal-to-baseline ratio (SBR) with limited resolution reporting subtle transients. We report herein development a suite sensors, designated NEMO, fast kinetics wide dynamic ranges (>100-fold). NEMO Ca2+ transients SBRs around 20-fold larger than top-of-the-range GCaMP6 series. sensors further enable quantification absolution concentration ratiometric or photochromic imaging. Compared GCaMP6s, NEMOs could detect single action potentials neurons SBR two times higher median four vivo, thereby outperforming most existing state-of-the-art GECIs. Given their high sensitivity to signals, may find broad applications neuronal other Ca2+-modulated physiological processes both mammals plants.

Language: Английский

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Advanced human iPSC-based preclinical model for Parkinson’s disease with optogenetic alpha-synuclein aggregation

Cell stem cell, Journal Year: 2023, Volume and Issue: 30(7), P. 973 - 986.e11

Published: June 19, 2023

Language: Английский

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Interfacing with the Brain: How Nanotechnology Can Contribute

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

Interfacing artificial devices with the human brain is central goal of neurotechnology. Yet, our imaginations are often limited by currently available paradigms and technologies. Suggestions for brain-machine interfaces have changed over time, along technology. Mechanical levers cable winches were used to move parts during mechanical age. Sophisticated electronic wiring remote control arisen age, ultimately leading plug-and-play computer interfaces. Nonetheless, brains so complex that these visions, until recently, largely remained unreachable dreams. The general problem, thus far, most technology mechanically and/or electrically engineered, whereas a living, dynamic entity. As result, worlds difficult interface one another. Nanotechnology, which encompasses engineered solid-state objects integrated circuits, excels at small length scales single few hundred nanometers and, thus, matches sizes biomolecules, biomolecular assemblies, cells. Consequently, we envision nanomaterials nanotools as opportunities in alternative ways. Here, review existing literature on use nanotechnology look forward discussing perspectives limitations based authors' expertise across range complementary disciplines─from neuroscience, engineering, physics, chemistry biology medicine, science mathematics, social jurisprudence. We focus but also include information from related fields when useful complementary.

Language: Английский

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Programming Bacteria With Light—Sensors and Applications in Synthetic Biology

Frontiers in Microbiology, Journal Year: 2018, Volume and Issue: 9

Published: Nov. 8, 2018

Photo-receptors are widely present in both prokaryotic and eukaryotic cells, which serves as the foundation of tuning cell behaviors with light. While practices cells have been relatively established, trials bacterial only emerging past few years. A number light sensors engineered bacteria most them fall into categories two-component one-component systems. Such a sensor toolbox has enabled controlling synthetic circuits at level transcription protein activity is major topic biology according to central dogma. Additionally, served for achieving based real-time feedback control. Here we review programming light, introducing their applications, including controls over microbial culture.

Language: Английский

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Towards circuit optogenetics

Current Opinion in Neurobiology, Journal Year: 2018, Volume and Issue: 50, P. 179 - 189

Published: April 7, 2018

Language: Английский

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