Co-variations of cerebral blood volume and single neurons discharge during resting state and visual cognitive tasks in non-human primates DOI Creative Commons

Julien Claron,

Matthieu Provansal, Quentin Salardaine

et al.

Cell Reports, Journal Year: 2023, Volume and Issue: 42(4), P. 112369 - 112369

Published: April 1, 2023

To better understand how the brain allows primates to perform various sets of tasks, ability simultaneously record neural activity at multiple spatiotemporal scales is challenging but necessary. However, contribution single-unit activities (SUAs) neurovascular remains be fully understood. Here, we combine functional ultrasound imaging cerebral blood volume (CBV) and SUA recordings in visual fronto-medial cortices behaving macaques. We show that provides a significant estimate response below typical fMRI spatial resolution 2mm3. Furthermore, our results also SUAs CBV are statistically uncorrelated during resting state correlate tasks. These have important implications for interpreting findings while one constructs inferences or

Language: Английский

Ultrasound Technologies for Imaging and Modulating Neural Activity DOI Creative Commons
Claire Rabut, Sangjin Yoo, Robert C. Hurt

et al.

Neuron, Journal Year: 2020, Volume and Issue: 108(1), P. 93 - 110

Published: Oct. 1, 2020

Language: Английский

Citations

189
Functional Ultrasound Imaging: A New Imaging Modality for Neuroscience DOI Creative Commons
Thomas Deffieux, Charlie Demené, Mickaël Tanter

et al.

Neuroscience, Journal Year: 2021, Volume and Issue: 474, P. 110 - 121

Published: March 13, 2021

Ultrasound sensitivity to slow blood flow motion gained two orders of magnitude in the last decade thanks advent ultrafast ultrasound imaging at thousands frames per second. In neuroscience, this access small cerebral vessels led introduction as a new and full-fledged neuroimaging modality. Much functional MRI or optical imaging, (fUS) takes benefit neurovascular coupling. Its ease use, portability, spatial temporal resolution makes it an attractive tool for brain activity preclinical imaging. A large fast-growing number studies wide variety animal models have demonstrated its potential neuroscience research. Beyond first proof concept applications humans are promising proved clear clinical interest particular human neonates, per-operative surgery, even development non-invasive machine interfaces.

Language: Английский

Citations

93
Functional Ultrasound (fUS) During Awake Brain Surgery: The Clinical Potential of Intra-Operative Functional and Vascular Brain Mapping DOI Creative Commons
Sadaf Soloukey, Arnaud J.P.E. Vincent, Djaina Satoer

et al.

Frontiers in Neuroscience, Journal Year: 2020, Volume and Issue: 13

Published: Jan. 8, 2020

Oncological neurosurgery relies heavily on making continuous, intra-operative tumor-brain delineations based image-guidance. Limitations of currently available imaging techniques call for the development real-time image-guided resection tools, which allow reliable functional and anatomical information in an setting. Functional ultrasound (fUS), is a new mobile neuro-imaging tool with unprecedented spatiotemporal resolution, allows detection small changes blood dynamics that reflect metabolic activity activated neurons through neurovascular coupling. We have applied fUS during conventional awake brain surgery to determine its clinical potential both vascular mapping, ultimate aim achieving maximum safe tumor resection. During surgery, was used image vasculature task-evoked activation electrocortical stimulation mapping (ESM) as gold standard. For imaging, patients were presented motor, language or visual tasks, while probe placed over (ESM-defined) areas. tissue (pre-resection) cavity (post-resection) imaged by moving hand-held along continuous trajectory regions interest. A total 10 included, predominantly intra-parenchymal frontal temporal lobe tumors low higher histopathological grades. able detect areas deep inside range tasks including processing. Brain could be at spatial resolution 300 μm 1.5-2.0 ms respectively, revealing tumor-specific, healthy characteristics. The current study presents applying surgery. illustrate relevance ability capture cortical responses well differences characteristics between tissue. As neurosurgical practice still pre-dominantly leaning inherently limited pre-operative resection-guidance, enters scene promising alternative anatomically physiologically informative.

Language: Английский

Citations

88
Whole-brain functional ultrasound imaging in awake head-fixed mice DOI
Clément Brunner, Micheline Grillet, Alan Urban

et al.

Nature Protocols, Journal Year: 2021, Volume and Issue: 16(7), P. 3547 - 3571

Published: June 4, 2021

Language: Английский

Citations

84
A Platform for Brain-wide Volumetric Functional Ultrasound Imaging and Analysis of Circuit Dynamics in Awake Mice DOI Creative Commons
Clément Brunner, Micheline Grillet, Arnau Sans-Dublanc

et al.

Neuron, Journal Year: 2020, Volume and Issue: 108(5), P. 861 - 875.e7

Published: Oct. 19, 2020

Language: Английский

Citations

78
Transfer functions linking neural calcium to single voxel functional ultrasound signal DOI Creative Commons
Ali-Kemal Aydin, William D. Haselden, Yannick Goulam Houssen

et al.

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: June 11, 2020

Functional ultrasound imaging (fUS) is an emerging technique that detects changes of cerebral blood volume triggered by brain activation. Here, we investigate the extent to which fUS faithfully reports local neuronal activation combining and two-photon microscopy (2PM) in a co-registered single voxel volume. Using machine-learning approach, compute validate transfer functions between dendritic calcium signals specific neurons vascular measured at both microscopic mesoscopic levels. We find are robust across wide range stimulation paradigms animals, reveal second component neurovascular coupling upon very strong stimulation. propose can be considered as reliable quantitative reporters follow dynamics.

Language: Английский

Citations

76
BRICseq Bridges Brain-wide Interregional Connectivity to Neural Activity and Gene Expression in Single Animals DOI Creative Commons
Longwen Huang, Justus M. Kebschull, Daniel Fürth

et al.

Cell, Journal Year: 2020, Volume and Issue: 182(1), P. 177 - 188.e27

Published: July 1, 2020

Language: Английский

Citations

76
Localized semi-nonnegative matrix factorization (LocaNMF) of widefield calcium imaging data DOI Creative Commons
Shreya Saxena,

Ian Kinsella,

Simon Musall

et al.

PLoS Computational Biology, Journal Year: 2020, Volume and Issue: 16(4), P. e1007791 - e1007791

Published: April 13, 2020

Widefield calcium imaging enables recording of large-scale neural activity across the mouse dorsal cortex. In order to examine relationship these signals resulting behavior, it is critical demix recordings into meaningful spatial and temporal components that can be mapped onto well-defined brain regions. However, no current tools satisfactorily extract different regions in individual mice a data-driven manner, while taking account mouse-specific preparation-specific differences. Here, we introduce Localized semi-Nonnegative Matrix Factorization (LocaNMF), method efficiently decomposes widefield video data allows us directly compare multiple by outputting localized functional are significantly more interpretable than traditional decomposition techniques. Moreover, provides natural subspace correlation maps dynamics behaviors, mice, experimental conditions, identification task- movement-related

Language: Английский

Citations

75
Neural correlates of blood flow measured by ultrasound DOI Creative Commons
Anwar O. Núñez-Elizalde, Michael Krumin, Charu Bai Reddy

et al.

Neuron, Journal Year: 2022, Volume and Issue: 110(10), P. 1631 - 1640.e4

Published: March 11, 2022

Functional ultrasound imaging (fUSI) is an appealing method for measuring blood flow and thus infer brain activity, but it relies on the physiology of neurovascular coupling requires extensive signal processing. To establish to what degree fUSI trial-by-trial signals reflect neural we performed simultaneous recordings with Neuropixels probes in awake mice. strongly correlated slow (<0.3 Hz) fluctuations local firing rate were closely predicted by smoothed neurons, particularly putative inhibitory neurons. The optimal smoothing filter had a width ∼3 s, matched hemodynamic response function mice, was invariant across mice stimulus conditions, similar cortex hippocampus. also spatially: rates as highly hemispheres signals. Thus, measured bears simple accurate relationship neuronal firing.

Language: Английский

Citations

56
Pyramidal cell types drive functionally distinct cortical activity patterns during decision-making DOI Creative Commons
Simon Musall, Xiaonan Richard Sun, Hemanth Mohan

et al.

Nature Neuroscience, Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 23, 2023

Abstract Understanding how cortical circuits generate complex behavior requires investigating the cell types that comprise them. Functional differences across pyramidal neuron (PyN) have been observed within areas, but it is not known whether these local extend throughout cortex, nor additional emerge when larger-scale dynamics are considered. We used genetic and retrograde labeling to target tract, intratelencephalic corticostriatal projection neurons measured their cortex-wide activity. Each PyN type drove unique neural dynamics, both at scales. Cortical activity optogenetic inactivation during an auditory decision task revealed distinct functional roles. All PyNs in parietal cortex were recruited perception of stimulus, but, surprisingly, tract had largest causal role. In frontal all required for accurate choices showed choice tuning. Our results reveal rich, cell-type-specific shape perceptual decisions.

Language: Английский

Citations

38