Spatial Comparisons of Mechanosensory Information Govern the Grooming Sequence in Drosophila DOI Creative Commons
Neil Zhang, Li Guo, J. Simpson

et al.

Current Biology, Journal Year: 2020, Volume and Issue: 30(6), P. 988 - 1001.e4

Published: March 1, 2020

Animals integrate information from different sensory modalities, body parts, and time points to inform behavioral choice, but the relevant comparisons underlying neural circuits are still largely unknown. We use grooming behavior of Drosophila melanogaster as a model investigate that govern motor sequence. Flies perform movements spontaneously, when covered with dust, they clean their bodies following an anterior-to-posterior After investigating modalities could detect we focus on mechanosensory bristle neurons, whose optogenetic activation induces similar Computational modeling predicts higher input strength head will cause anterior occur first. test this prediction using competition assay whereby two targeted light beams independently activate neurons parts. find initial choice movement is determined by ratio inputs In dust-covered flies, change result successful cleaning movements. Simulations our suggest results in sequence progression. One possibility flies frequent between posterior inputs, changing ratios drive choices. Alternatively, may track temporal given part measure effectiveness. The first hypothesis supported experiments: iterative spatial parts essential for organizing

Language: Английский

Neural network organization for courtship-song feature detection in Drosophila DOI Creative Commons
Christa A. Baker, Claire McKellar, Rich Pang

et al.

Current Biology, Journal Year: 2022, Volume and Issue: 32(15), P. 3317 - 3333.e7

Published: July 5, 2022

Animals communicate using sounds in a wide range of contexts, and auditory systems must encode behaviorally relevant acoustic features to drive appropriate reactions. How feature detection emerges along pathways has been difficult solve due challenges mapping the underlying circuits characterizing responses features. Here, we study activity Drosophila melanogaster brain investigate selectivity for two main modes fly courtship song, sinusoids pulse trains. We identify 24 new cell types intermediate layers pathway, connectomic resource, FlyWire, map all synaptic connections between these types, addition known early higher-order neurons-this represents first circuit-level pathway. additionally determine sign (excitatory or inhibitory) most synapses this connectome. find that neurons display continuum preferences song with different song-mode response timescales are highly interconnected network lacks hierarchical structure. Nonetheless, properties individual within connectome predictable from their inputs. Our thus provides insights into organization coding brain.

Language: Английский

Citations

47
Neural circuit mechanisms for transforming learned olfactory valences into wind-oriented movement DOI Creative Commons
Yoshinori Aso, Daichi Yamada, Daniel Bushey

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Sept. 18, 2023

How memories are used by the brain to guide future action is poorly understood. In olfactory associative learning in Drosophila, multiple compartments of mushroom body act parallel assign a valence stimulus. Here, we show that appetitive stored different induce levels upwind locomotion. Using photoactivation screen new collection split-GAL4 drivers and EM connectomics, identified cluster neurons postsynaptic output (MBONs) can trigger robust steering. These UpWind Neurons (UpWiNs) integrate inhibitory excitatory synaptic inputs from MBONs aversive memory compartments, respectively. After formation memory, UpWiNs acquire enhanced response reward-predicting odors as presynaptic MBON undergoes depression. Blocking impaired reduced locomotion during retrieval. Photoactivation also increased chance returning location where activation was terminated, suggesting an additional role navigation. Thus, our results provide insight into how learned abstract valences gradually transformed concrete memory-driven actions through divergent convergent networks, neuronal architecture commonly found vertebrate invertebrate brains.

Language: Английский

Citations

30
Olfactory navigation in arthropods DOI Creative Commons

Theresa J. Steele,

Aaron J. Lanz, Katherine I. Nagel

et al.

Journal of Comparative Physiology A, Journal Year: 2023, Volume and Issue: 209(4), P. 467 - 488

Published: Jan. 20, 2023

Abstract Using odors to find food and mates is one of the most ancient highly conserved behaviors. Arthropods from flies moths crabs use broadly similar strategies navigate toward odor sources—such as integrating flow information with information, comparing concentration across sensors, over time. Because arthropods share many homologous brain structures—antennal lobes for processing olfactory mechanosensors flow, mushroom bodies (or hemi-ellipsoid bodies) associative learning, central complexes navigation, it likely that these closely related behaviors are mediated by neural circuits. However, differences in types they seek, physics dispersal, locomotion water, air, on substrates mean circuits must have adapted generate a wide diversity odor-seeking In this review, we discuss common specializations observed navigation behavior arthropods, review our current knowledge about subserving behavior. We propose comparative study arthropod nervous systems may provide insight into how set basic circuit structures has diversified different environments.

Language: Английский

Citations

24
Spatial readout of visual looming in the central brain of Drosophila DOI Creative Commons
Mai M Morimoto, Aljoscha Nern, Arthur Zhao

et al.

eLife, Journal Year: 2020, Volume and Issue: 9

Published: Nov. 18, 2020

Visual systems can exploit spatial correlations in the visual scene by using retinotopy, organizing principle which neighboring cells encode locations. However, retinotopy is often lost, such as when pathways are integrated with other sensory modalities. How information processed outside of strictly brain areas? Here, we focused on looming responsive LC6 Drosophila, a population whose dendrites collectively cover field, but axons form single glomerulus-a structure without obvious retinotopic organization-in central brain. We identified multiple cell types downstream glomerulus and found that they more strongly respond to different portions unexpectedly preserving information. Through EM reconstruction all synaptic inputs glomerulus, circuits within enable readout features contralateral suppression-mechanisms transform for behavioral control.

Language: Английский

Citations

56
Encoding and control of orientation to airflow by a set of Drosophila fan-shaped body neurons DOI Creative Commons
Timothy A. Currier, Andrew M. M. Matheson, Katherine I. Nagel

et al.

eLife, Journal Year: 2020, Volume and Issue: 9

Published: Dec. 30, 2020

The insect central complex (CX) is thought to underlie goal-oriented navigation but its functional organization not fully understood. We recorded from genetically-identified CX cell types in Drosophila and presented directional visual, olfactory, airflow cues known elicit orienting behavior. found that a group of neurons targeting the ventral fan-shaped body (ventral P-FNs) are robustly tuned for direction. Ventral P-FNs did generate ‘map’ Instead, cells each hemisphere were 45° ipsilateral, forming pair orthogonal bases. Imaging experiments suggest inherit their tuning provide input lateral accessory lobe (LAL) noduli (NO). Silencing prevented flies selecting appropriate corrective turns following changes Our results identify encode direction required proper orientation this stimulus.

Language: Английский

Citations

55
A connectome of theDrosophilacentral complex reveals network motifs suitable for flexible navigation and context-dependent action selection DOI Creative Commons
Brad K. Hulse, Hannah Haberkern, Romain Franconville

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2020, Volume and Issue: unknown

Published: Dec. 9, 2020

ABSTRACT Flexible behaviors over long timescales are thought to engage recurrent neural networks in deep brain regions, which experimentally challenging study. In insects, circuit dynamics a region called the central complex (CX) enable directed locomotion, sleep, and context- experience-dependent spatial navigation. We describe first complete electron-microscopy-based connectome of Drosophila CX, including all its neurons circuits at synaptic resolution. identified new CX neuron types, novel sensory motor pathways, network motifs that likely extract fly’s head-direction, maintain it with attractor dynamics, combine other sensorimotor information perform vector-based navigational computations. also numerous pathways may facilitate selection CX-driven behavioral patterns by context internal state. The provides comprehensive blueprint necessary for detailed understanding underlying flexible navigation, state-dependent action selection.

Language: Английский

Citations

50
A unified mechanism for innate and learned visual landmark guidance in the insect central complex DOI Creative Commons
Roman Goulard, Cornelia Buehlmann, Jeremy E. Niven

et al.

PLoS Computational Biology, Journal Year: 2021, Volume and Issue: 17(9), P. e1009383 - e1009383

Published: Sept. 23, 2021

Insects can navigate efficiently in both novel and familiar environments, this requires flexiblity how they are guided by sensory cues. A prominent landmark, for example, elicit strong innate behaviours (attraction or menotaxis) but also be used, after learning, as a specific directional cue part of navigation memory. However, the mechanisms that allow pathways to co-exist, interact override each other largely unknown. Here we propose model behavioural integration learned guidance based on neuroanatomy central complex (CX), adapted control landmark behaviours. We consider reward signal provided either an attraction landmarks long-term visual memory mushroom bodies (MB) modulates formation local vector CX. Using operant strategy simulated agent exploring simple world containing single cue, show generated short-term support steering behaviour. In addition, architecture is consistent with observed effects unilateral MB lesions ants cause reversion suggest CX interpreted transforming rewarding (positive negative) signals into mapping environment describes geometrical attractiveness (or repulsion). discuss scheme might represent ideal way combine multisensory information gathered during exploration optimal integration.

Language: Английский

Citations

42
Behavioral signatures of structured feature detection during courtship in Drosophila DOI Creative Commons
Jing Ning, Zhou Li,

Xingjiang Zhang

et al.

Current Biology, Journal Year: 2022, Volume and Issue: 32(6), P. 1211 - 1231.e7

Published: Feb. 8, 2022

Language: Английский

Citations

30
Lineages to circuits: the developmental and evolutionary architecture of information channels into the central complex DOI Creative Commons

Pratyush Kandimalla,

Jaison J. Omoto, Elizabeth J. Hong

et al.

Journal of Comparative Physiology A, Journal Year: 2023, Volume and Issue: 209(4), P. 679 - 720

Published: March 17, 2023

The representation and integration of internal external cues is crucial for any organism to execute appropriate behaviors. In insects, a highly conserved region the brain, central complex (CX), functions in spatial information behavioral states, as well transformation this into desired navigational commands. How does relatively invariant structure enable incorporation from diversity anatomical, behavioral, ecological niches occupied by insects? Here, we examine input channels CX context their development evolution. Insect brains develop ~ 100 neuroblasts per hemisphere that divide systematically form "lineages" sister neurons, project target neuropils along anatomically characteristic tracts. Overlaying developmental tract onto recently generated Drosophila "hemibrain" connectome integrating with anatomical physiological recording neurons other species, observe neuropil lineage-specific innervation, connectivity, activity profiles channels. We posit proliferative potential lineage-based architecture modification neural networks across existing, novel, deprecated modalities species-specific manner, thus forming substrate evolution diversification insect circuits.

Language: Английский

Citations

21
SAMPL is a high-throughput solution to study unconstrained vertical behavior in small animals DOI Creative Commons
Yunlu Zhu, Franziska Auer, Hannah Gelnaw

et al.

Cell Reports, Journal Year: 2023, Volume and Issue: 42(6), P. 112573 - 112573

Published: June 1, 2023

Balance and movement are impaired in many neurological disorders. Recent advances behavioral monitoring provide unprecedented access to posture locomotor kinematics but without the throughput scalability necessary screen candidate genes/potential therapeutics. Here, we present a scalable apparatus measure locomotion (SAMPL). SAMPL includes extensible hardware open-source software with real-time processing can acquire data from D. melanogaster, C. elegans, rerio as they move vertically. Using SAMPL, define how zebrafish balance navigate vertically discover small systematic variations among kinematic parameters between genetic backgrounds. We demonstrate SAMPL's ability resolve differences navigation function of effect size gathered, providing key for screens. is therefore both tool model disorders an exemplar scale support

Language: Английский

Citations

20