Circadian clock disruption promotes the degeneration of dopaminergic neurons in male Drosophila DOI Creative Commons
Michaëla Majcin Dorcikova,

Lou C. Duret,

Emma Pottié

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Sept. 22, 2023

Abstract Sleep and circadian rhythm disruptions are frequent comorbidities of Parkinson’s disease (PD), a disorder characterized by the progressive loss dopaminergic (DA) neurons in substantia nigra. However, causal role clocks degenerative process remains uncertain. We demonstrated here that regulate rhythmicity magnitude vulnerability DA to oxidative stress male Drosophila . Circadian pacemaker presynaptic subset rhythmically modulate their susceptibility degeneration. The arrhythmic period ( per ) gene null mutation exacerbates age-dependent and, combination with brief stress, causes premature animal death. These findings suggest clock disruption promotes neurodegeneration.

Language: Английский

A connectome of the Drosophila central complex reveals network motifs suitable for flexible navigation and context-dependent action selection DOI Creative Commons
Brad K. Hulse, Hannah Haberkern, Romain Franconville

et al.

eLife, Journal Year: 2021, Volume and Issue: 10

Published: Oct. 26, 2021

Flexible behaviors over long timescales are thought to engage recurrent neural networks in deep brain regions, which experimentally challenging study. In insects, circuit dynamics a region called the central complex (CX) enable directed locomotion, sleep, and context- experience-dependent spatial navigation. We describe first complete electron microscopy-based connectome of

Language: Английский

Citations

301
The Regulation of Drosophila Sleep DOI Creative Commons
Orie T. Shafer, Alex C. Keene

Current Biology, Journal Year: 2021, Volume and Issue: 31(1), P. R38 - R49

Published: Jan. 1, 2021

Language: Английский

Citations

166
Pushing the frontiers: tools for monitoring neurotransmitters and neuromodulators DOI
Zhaofa Wu, Dayu Lin, Yulong Li

et al.

Nature reviews. Neuroscience, Journal Year: 2022, Volume and Issue: 23(5), P. 257 - 274

Published: March 31, 2022

Language: Английский

Citations

156
A neural circuit architecture for rapid learning in goal-directed navigation DOI Creative Commons
Chuntao Dan, Brad K. Hulse,

Ramya Kappagantula

et al.

Neuron, Journal Year: 2024, Volume and Issue: 112(15), P. 2581 - 2599.e23

Published: May 24, 2024

Anchoring goals to spatial representations enables flexible navigation but is challenging in novel environments when both must be acquired simultaneously. We propose a framework for how Drosophila uses internal of head direction (HD) build goal upon selective thermal reinforcement. show that flies use stochastically generated fixations and directed saccades express heading preferences an operant visual learning paradigm HD neurons are required modify these based on used symmetric setting expose flies' co-evolve the reliability interacting impacts behavior. Finally, we describe rapid new headings may rest behavioral policy whose parameters form genetically encoded circuit architecture. Such evolutionarily structured architectures, which enable rapidly adaptive behavior driven by representations, relevant across species.

Language: Английский

Citations

17
Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation DOI Open Access
Tanya Wolff, Mark Eddison, Nan Chen

et al.

Published: Jan. 21, 2025

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent situ hybridization. About neuropeptides we examined were expressed only few cells, while rest dozens hundreds cells. Neuropeptide receptors more broadly at lower levels. Using our GAL4 drivers mark found 51 85 types least one 21 multiple neuropeptides. Surprisingly, all co-expressed molecule neurotransmitter. Finally, used identify whose activation affects sleep, identified other link circadian clock CX. well-characterized genetic information on neurotransmitter provide should enhance studies

Language: Английский

Citations

2
The Neuroanatomical Ultrastructure and Function of a Biological Ring Attractor DOI Creative Commons
Daniel B. Turner‐Evans, Kristopher T. Jensen,

Saba Ali

et al.

Neuron, Journal Year: 2020, Volume and Issue: 108(1), P. 145 - 163.e10

Published: Sept. 10, 2020

Language: Английский

Citations

134
A Neural Network for Wind-Guided Compass Navigation DOI Creative Commons

Tatsuo S. Okubo,

Paola Patella, Isabel D’Alessandro

et al.

Neuron, Journal Year: 2020, Volume and Issue: 107(5), P. 924 - 940.e18

Published: July 17, 2020

Spatial maps in the brain are most accurate when they linked to external sensory cues. Here, we show that compass Drosophila is direction of wind. Shifting wind rightward rotates as if fly were turning leftward, and vice versa. We describe mechanisms several computations integrate information into compass. First, an intensity-invariant representation computed by comparing left-right mechanosensory signals. Then, signals reformatted reduce coding biases inherent peripheral mechanics, cues brought same circular coordinate system represents visual self-motion Because incorporates both cues, it should enable navigation under conditions where no single cue consistently reliable. These results how local can be transformed a global, multimodal, abstract space.

Language: Английский

Citations

106
Model and Non-model Insects in Chronobiology DOI Creative Commons
Katharina Beer, Charlotte Helfrich‐Förster

Frontiers in Behavioral Neuroscience, Journal Year: 2020, Volume and Issue: 14

Published: Nov. 26, 2020

The fruit fly Drosophila melanogaster is an established model organism in chronobiology, because genetic manipulation and breeding the laboratory are easy. circadian clock neuroanatomy D. one of best-known networks insects basic behavior has been characterized detail this insect. Another chronobiology honey bee Apis mellifera , which diurnal foraging described already early twentieth century. A. hallmarks research on interplay between sociality complex behaviors like sun compass navigation time-place-learning. Nevertheless, there aspects structure function, for example role photoperiodism diapause, can be only insufficiently investigated these two models. Unlike high-latitude flies such as Chymomyza costata or ezoana cosmopolitan do not display a photoperiodic diapause. Similarly, bees go into “real” but most solitary species exhibit obligatory Furthermore, evolved different Hymenoptera independently, wherefore it might misleading to study social Consequently, additional non-model required understand Diptera Hymenoptera. In review, we introduce compare them with other show their advantages limitations general models insect clocks.

Language: Английский

Citations

91
Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicity DOI Creative Commons
Matthias Schlichting, Madelen M. Díaz,

Jason Xin

et al.

eLife, Journal Year: 2019, Volume and Issue: 8

Published: Oct. 15, 2019

Animal circadian rhythms persist in constant darkness and are driven by intracellular transcription-translation feedback loops. Although these cellular oscillators communicate, isolated mammalian clocks continue to tick away without intercellular communication. To investigate issues Drosophila, we assayed behavior as well molecular within individual brain clock neurons while blocking communication the ca. 150 neuron network. We also generated CRISPR-mediated neuron-specific knockouts. The results point two key groups: loss of both regions but neither one alone has a strong behavioral phenotype darkness; between contributes period determination. Under dark conditions, region persists network famous PDF-expressing s-LNv however was strongly dependent on communication, likely because gene expression vulnerable sLNvs depends neuronal firing or light.

Language: Английский

Citations

79
Drosophila clock cells use multiple mechanisms to transmit time-of-day signals in the brain DOI Open Access
Annika F. Barber, Shi Yi Fong,

Anna Kolesnik

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2021, Volume and Issue: 118(10)

Published: March 3, 2021

Significance Despite our growing understanding of how the fly clock network maintains free-running rhythms behavior and physiology, little is known about information communicated from to rest brain regulate behavior. We identify glutamate acetylcholine as key neurotransmitters signaling neurons pars intercerebralis (PI), a output region regulating circadian sleep metabolism. report link between Drosophila evening PI, find that effect on neuron physiology varies, suggesting same cells use multiple mechanisms simultaneously drive cycling in neurons.

Language: Английский

Citations

69