Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(8), P. 3672 - 3678
Published: Feb. 15, 2024
Redox
potentiometry
has
emerged
as
a
new
platform
for
in
vivo
sensing,
with
improved
neuronal
compatibility
and
strong
tolerance
against
sensitivity
variation
caused
by
protein
fouling.
Although
enzymes
show
great
possibilities
the
fabrication
of
selective
redox
potentiometry,
an
enzyme
electrode
to
output
open-circuit
voltage
(EOC)
fast
response
remains
challenging.
Herein,
we
report
concept
novel
enzymatic
galvanic
(GRP)
time
coupling
merits
high
selectivity
electrodes
excellent
biocompatibility
reliability
GRP
sensors.
With
glucose
biosensor
illustration,
use
flavin
adenine
dinucleotide-dependent
dehydrogenase
recognition
element
carbon
black
potential
relay
station
improve
time.
We
find
that
rapidly
responds
good
linear
relationship
between
EOC
logarithm
concentration
within
range
from
100
μM
2.65
mM.
The
shows
over
O2
coexisting
neurochemicals,
reversibility,
can
monitor
dynamics
rat
brain.
believe
this
study
will
pave
potentiometric
biosensing
chemical
events
reliability.
Neuron,
Journal Year:
2023,
Volume and Issue:
112(5), P. 718 - 739
Published: Dec. 15, 2023
Fiber
photometry
is
a
key
technique
for
characterizing
brain-behavior
relationships
in
vivo.
Initially,
it
was
primarily
used
to
report
calcium
dynamics
as
proxy
neural
activity
via
genetically
encoded
indicators.
This
generated
new
insights
into
brain
functions
including
movement,
memory,
and
motivation
at
the
level
of
defined
circuits
cell
types.
Recently,
opportunity
discovery
with
fiber
has
exploded
development
an
extensive
range
fluorescent
sensors
biomolecules
neuromodulators
peptides
that
were
previously
inaccessible
critical
advance,
combined
availability
affordable
"plug-and-play"
recording
systems,
made
monitoring
molecules
high
spatiotemporal
precision
during
behavior
highly
accessible.
However,
while
opening
exciting
avenues
research,
rapid
expansion
applications
occurred
without
coordination
or
consensus
on
best
practices.
Here,
we
provide
comprehensive
guide
help
end-users
execute,
analyze,
suitably
interpret
studies.
Molecular Psychiatry,
Journal Year:
2024,
Volume and Issue:
29(11), P. 3680 - 3693
Published: May 24, 2024
Abstract
A
wealth
of
neuromodulatory
transmitters
regulate
synaptic
circuits
in
the
brain.
Their
mode
signaling,
often
called
volume
transmission,
differs
from
classical
transmission
important
ways.
In
vesicles
rapidly
fuse
response
to
action
potentials
and
release
their
transmitter
content.
The
are
then
sensed
by
nearby
receptors
on
select
target
cells
with
minimal
delay.
Signal
is
restricted
contacts
typically
occurs
within
~1
ms.
Volume
doesn’t
rely
contact
sites
main
monoamines
neuropeptides,
neuromodulators
It
less
precise
than
underlying
molecular
mechanisms
spatiotemporal
scales
not
well
understood.
Here,
we
review
literature
raise
scientific
questions
that
should
be
addressed
years
ahead.
We
define
five
domains
which
systems
can
differ
one
another.
These
(1)
innervation
patterns
firing
properties,
(2)
synthesis
loading
into
different
types
vesicles,
(3)
architecture
distribution
sites,
(4)
diffusion,
degradation,
reuptake,
(5)
receptor
positioning
cells.
discuss
these
for
dopamine,
a
well-studied
monoamine,
compare
dopamine
norepinephrine
serotonin.
include
assessments
neuropeptide
signaling
central
acetylcholine
transmission.
Through
this
review,
provide
cellular
framework
This
mechanistic
knowledge
essential
how
control
behavior
health
disease
understand
they
modulated
medical
treatments
drugs
abuse.
Nature Neuroscience,
Journal Year:
2024,
Volume and Issue:
27(9), P. 1844 - 1857
Published: July 15, 2024
Abstract
Neuropeptides
are
ubiquitous
in
the
nervous
system.
Research
into
neuropeptides
has
been
limited
by
a
lack
of
experimental
tools
that
allow
for
precise
dissection
their
complex
and
diverse
dynamics
circuit-specific
manner.
Opioid
peptides
modulate
pain,
reward
aversion
as
such
have
high
clinical
relevance.
To
illuminate
spatiotemporal
endogenous
opioid
signaling
brain,
we
developed
class
genetically
encoded
fluorescence
sensors
based
on
kappa,
delta
mu
receptors:
κLight,
δLight
µLight,
respectively.
We
characterized
pharmacological
profiles
these
mammalian
cells
dissociated
neurons.
used
κLight
to
identify
electrical
stimulation
parameters
trigger
release
scale
dynorphin
volume
transmission
brain
slices.
Using
vivo
fiber
photometry
mice,
demonstrated
utility
detecting
optogenetically
driven
observed
differential
response
fearful
rewarding
conditions.
Frontiers in Integrative Neuroscience,
Journal Year:
2024,
Volume and Issue:
18
Published: Feb. 19, 2024
Bioelectronic
Medicine
stands
as
an
emerging
field
that
rapidly
evolves
and
offers
distinctive
clinical
benefits,
alongside
unique
challenges.
It
consists
of
the
modulation
nervous
system
by
precise
delivery
electrical
current
for
treatment
conditions,
such
post-stroke
movement
recovery
or
drug-resistant
disorders.
The
unquestionable
impact
is
underscored
successful
translation
to
humans
in
last
decades,
long
list
preclinical
studies.
Given
emergency
accelerating
progress
new
neuromodulation
treatments
(i.e.,
hypertension,
autoimmune
degenerative
diseases),
collaboration
between
multiple
fields
imperative.
This
work
intends
foster
multidisciplinary
bring
together
different
provide
fundamental
basis
underlying
Medicine.
In
this
review
we
will
go
from
biophysics
cell
membrane,
which
consider
inner
core
neuromodulation,
patient
care.
We
discuss
recently
discovered
mechanism
neurotransmission
switching
how
it
design,
update
on
neuronal
glial
health
disease.
advances
biomedical
technology
have
facilitated
collection
large
amounts
data,
thereby
introducing
challenges
data
analysis.
approaches
high
throughput
analysis,
encompassing
big
networks,
artificial
intelligence,
internet
things.
Emphasis
be
placed
understanding
electrochemical
properties
neural
interfaces,
along
with
integration
biocompatible
reliable
materials
compliance
regulations
translational
applications.
Preclinical
validation
foundational
process,
critical
aspects
animal
Finally,
focus
point-of-care
ultimate
goal
bioelectronic
medicine.
a
call
scientists
common
endeavor:
accelerate
decoding
era
therapeutic
possibilities.
Advanced Materials,
Journal Year:
2022,
Volume and Issue:
35(11)
Published: Dec. 7, 2022
Integration
of
plasmonic
nanostructures
with
fiber-optics-based
neural
probes
enables
label-free
detection
molecular
fingerprints
via
surface-enhanced
Raman
spectroscopy
(SERS),
and
it
represents
a
fascinating
technological
horizon
to
investigate
brain
function.
However,
developing
neuroplasmonic
that
can
interface
deep
regions
minimal
invasiveness
while
providing
the
sensitivity
detect
biomolecular
signatures
in
physiological
environment
is
challenging,
particular
because
same
waveguide
must
be
employed
for
both
delivering
excitation
light
collecting
resulting
scattered
photons.
Here,
SERS-active
probe
based
on
tapered
optical
fiber
(TF)
decorated
gold
nanoislands
(NIs)
neurotransmitters
down
micromolar
range
presented.
To
do
this,
novel,
nonplanar
repeated
dewetting
technique
fabricate
NIs
sub-10
nm
gaps,
uniformly
distributed
wide
(square
millimeter
scale
surface
area),
highly
curved
TF
developed.
It
experimentally
numerically
shown
amplified
broadband
near-field
enhancement
high-density
layer
allows
achieving
limit
aqueous
solution
10-7
m
rhodamine
6G
10-5
serotonin
dopamine
through
SERS
at
near-infrared
wavelengths.
The
NIs-TF
technology
envisioned
as
first
step
toward
unexplored
frontier
vivo
interfaces.
