The synaptic basis of activity-dependent eye-specific competition DOI Creative Commons
Chenghang Zhang,

Swapnil Yadav,

Colenso M. Speer

et al.

Cell Reports, Journal Year: 2023, Volume and Issue: 42(2), P. 112085 - 112085

Published: Feb. 1, 2023

Binocular vision requires proper developmental wiring of eye-specific inputs to the brain. In thalamus, axons from two eyes initially overlap in dorsal lateral geniculate nucleus and undergo activity-dependent competition segregate into target domains. Here, we combine tract tracing with volumetric super-resolution imaging measure nanoscale molecular reorganization developing retinogeniculate synapses mouse We show there are differences presynaptic vesicle pool size association active zone at earliest stages refinement but find no evidence subsynaptic domain number, size, or transsynaptic alignment across development. Genetic disruption spontaneous retinal activity decreases synapse density, delays emergence organization, disrupts maturation. These results suggest that maturation underlies synaptic mammalian visual system.

Language: Английский

The effects of N6-methyladenosine RNA methylation on the nervous system DOI
Nan Shao,

Ting Ye,

Wei-ting Xuan

et al.

Molecular and Cellular Biochemistry, Journal Year: 2023, Volume and Issue: 478(12), P. 2657 - 2669

Published: March 10, 2023

Language: Английский

Citations

8
Huntingtin recruits KIF1A to transport synaptic vesicle precursors along the mouse axon to support synaptic transmission and motor skill learning DOI Creative Commons
Hélène Vitet,

Julie Bruyère,

Hao Xu

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: July 11, 2023

Neurotransmitters are released at synapses by synaptic vesicles (SVs), which originate from SV precursors (SVPs) that have traveled along the axon. Because each synapse maintains a pool of SVs, only small fraction released, it has been thought axonal transport SVPs does not affect function. Here, studying corticostriatal network both in microfluidic devices and mice, we find phosphorylation Huntingtin protein (HTT) increases glutamate release recruiting kinesin motor KIF1A. In constitutive HTT causes over-accumulation synapses, probability release, impairs skill learning on rotating rod. Silencing KIF1A these mice restored to wild-type levels. Axonal SVP within thus influences plasticity learning.

Language: Английский

Citations

8
End-binding protein 1 promotes specific motor-cargo association in the cell body prior to axonal delivery of dense core vesicles DOI Creative Commons
Junhyun Park, Yi Xie, Kenneth G. Miller

et al.

Current Biology, Journal Year: 2023, Volume and Issue: 33(18), P. 3851 - 3864.e7

Published: Aug. 15, 2023

Language: Английский

Citations

8
The Roles of Par3, Par6, and aPKC Polarity Proteins in Normal Neurodevelopment and in Neurodegenerative and Neuropsychiatric Disorders DOI Open Access
Lili Zhang,

Xiangyun Wei

Journal of Neuroscience, Journal Year: 2022, Volume and Issue: 42(24), P. 4774 - 4793

Published: June 15, 2022

Normal neural circuits and functions depend on proper neuronal differentiation, migration, synaptic plasticity, maintenance. Abnormalities in these processes underlie various neurodevelopmental, neuropsychiatric, neurodegenerative disorders. Neural development maintenance are regulated by many proteins. Among them Par3, Par6 (partitioning defective 3 6), aPKC (atypical protein kinase C) families of evolutionarily conserved polarity These proteins perform versatile forming tripartite or other combinations complexes, which hereafter collectively referred to as "Par complexes." In this review, we summarize the major findings their biophysical biochemical properties cell polarization signaling pathways. We next expression localization nervous system well aspects neurodevelopment, including neuroepithelial polarity, neurogenesis, neurite memory. rely fundamental roles Par complexes distinct cellular contexts. also discuss how may correlate with subcellular neurons. Finally, review involvement neuropsychiatric disorders, such schizophrenia Alzheimer9s disease. While emerging evidence indicates that essential for maintenance, questions in vivo have yet be answered. Thus, Par6, continue important research topics advance neuroscience.

Language: Английский

Citations

12
The synaptic basis of activity-dependent eye-specific competition DOI Creative Commons
Chenghang Zhang,

Swapnil Yadav,

Colenso M. Speer

et al.

Cell Reports, Journal Year: 2023, Volume and Issue: 42(2), P. 112085 - 112085

Published: Feb. 1, 2023

Binocular vision requires proper developmental wiring of eye-specific inputs to the brain. In thalamus, axons from two eyes initially overlap in dorsal lateral geniculate nucleus and undergo activity-dependent competition segregate into target domains. Here, we combine tract tracing with volumetric super-resolution imaging measure nanoscale molecular reorganization developing retinogeniculate synapses mouse We show there are differences presynaptic vesicle pool size association active zone at earliest stages refinement but find no evidence subsynaptic domain number, size, or transsynaptic alignment across development. Genetic disruption spontaneous retinal activity decreases synapse density, delays emergence organization, disrupts maturation. These results suggest that maturation underlies synaptic mammalian visual system.

Language: Английский

Citations

6