Genotypic sex shapes maternal care in the African pygmy mouse, Mus minutoides DOI
Louise D. Heitzmann,

Marie Challe,

Julie Pérez

et al.

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2023, Volume and Issue: 290(2006)

Published: Sept. 6, 2023

Sexually dimorphic behaviours, such as parental care, have long been thought to be mainly driven by gonadal hormones. In the past two decades, a few studies challenged this view, highlighting direct influence of sex chromosome complement (XX versus XY or ZZ ZW). The African pygmy mouse, Mus minutoides , is wild mouse species with naturally occurring reversal induced third, feminizing X* chromosome, leading three female genotypes: XX, XX* and X*Y. Here, we show that in X*Y females shapes divergent maternal care strategy (maternal aggression, pup retrieval nesting behaviours) from both XX females. Although neuroanatomical investigations were inconclusive, dopaminergic system anteroventral periventricular nucleus hypothalamus worth investigating further it may support differences behaviour between Combining behaviours neurobiology rodent subject natural selection, evaluate potential candidates for neural basis strengthen underestimated role chromosomes shaping brain behaviours. All things considered, highlight emergence third sexual phenotype, challenging binary view phenotypic sexes.

Language: Английский

The neuropeptidergic connectome of C. elegans DOI Creative Commons
Lidia Ripoll-Sánchez, Jan Watteyne, HaoSheng Sun

et al.

Neuron, Journal Year: 2023, Volume and Issue: 111(22), P. 3570 - 3589.e5

Published: Nov. 1, 2023

Efforts are ongoing to map synaptic wiring diagrams, or connectomes, understand the neural basis of brain function. However, chemical synapses represent only one type functionally important neuronal connection; in particular, extrasynaptic, "wireless" signaling by neuropeptides is widespread and plays essential roles all nervous systems. By integrating single-cell anatomical gene-expression datasets with biochemical analysis receptor-ligand interactions, we have generated a draft connectome neuropeptide C. elegans system. This network characterized high connection density, extended cascades, autocrine foci, decentralized topology, large, highly interconnected core containing three constituent communities sharing similar patterns input connectivity. Intriguingly, several key hubs little-studied neurons that appear specialized for peptidergic neuromodulation. We anticipate neuropeptidergic will serve as prototype how networks neuromodulatory organized.

Language: Английский

Citations

92
Hormone-mediated neural remodeling orchestrates parenting onset during pregnancy DOI
Rachida Ammari, Francesco Monaca,

Mingran Cao

et al.

Science, Journal Year: 2023, Volume and Issue: 382(6666), P. 76 - 81

Published: Oct. 6, 2023

During pregnancy, physiological adaptations prepare the female body for challenges of motherhood. Becoming a parent also requires behavioral adaptations. Such can occur as early during but how pregnancy hormones remodel parenting circuits to instruct preparatory changes remains unknown. We found that action estradiol and progesterone on galanin (Gal)–expressing neurons in mouse medial preoptic area (MPOA) is critical pregnancy-induced parental behavior. Whereas silences MPOA Gal paradoxically increases their excitability, permanently rewires this circuit node by promoting dendritic spine formation recruitment excitatory synaptic inputs. This -specific neural remodeling sparsens population activity vivo results persistently stronger, more selective responses pup stimuli. Pregnancy thus anticipation future need.

Language: Английский

Citations

43
Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples DOI Creative Commons
Nikita Vladimirov, Fabian F. Voigt, Thomas Naert

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 27, 2024

Abstract In 2015, we launched the mesoSPIM initiative, an open-source project for making light-sheet microscopy of large cleared tissues more accessible. Meanwhile, demand imaging larger samples at higher speed and resolution has increased, requiring major improvements in capabilities such microscopes. Here, introduce next-generation (“Benchtop”) with a significantly increased field view, improved resolution, throughput, affordable cost, simpler assembly compared to original version. We develop optical method testing detection objectives that enables us select optimal large-sensor cameras. The achieves high spatial (1.5 µm laterally, 3.3 axially) across entire magnification up 20×, supports sample sizes ranging from sub-mm several centimeters while being compatible multiple clearing techniques. microscope serves broad range applications neuroscience, developmental biology, pathology, even physics.

Language: Английский

Citations

17
Virtual reality-empowered deep-learning analysis of brain cells DOI Creative Commons
Doris Kaltenecker, Rami Al-Maskari, Moritz Negwer

et al.

Nature Methods, Journal Year: 2024, Volume and Issue: 21(7), P. 1306 - 1315

Published: April 22, 2024

Abstract Automated detection of specific cells in three-dimensional datasets such as whole-brain light-sheet image stacks is challenging. Here, we present DELiVR, a virtual reality-trained deep-learning pipeline for detecting c-Fos + markers neuronal activity cleared mouse brains. Virtual reality annotation substantially accelerated training data generation, enabling DELiVR to outperform state-of-the-art cell-segmenting approaches. Our available user-friendly Docker container that runs with standalone Fiji plugin. features comprehensive toolkit visualization and can be customized other cell types interest, did here microglia somata, using dataset-specific training. We applied investigate cancer-related brain activity, unveiling an activation pattern distinguishes weight-stable cancer from cancers associated weight loss. Overall, robust tool does not require advanced coding skills analyze imaging health disease.

Language: Английский

Citations

14
Midbrain ghrelin receptor signalling regulates binge drinking in a sex specific manner DOI Creative Commons

Amy J. Pearl,

Xavier J. Maddern,

Paulo Pinares-Garcia

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 15, 2025

Abstract Risky drinking rates are rising, particularly in women, yet sex as a biological variable has only recently gained traction. The centrally projecting Edinger-Westphal (EWcp) nucleus emerged key regulator of alcohol consumption. Here we found that EWcp peptidergic cells reduce binge specifically female mice. We show this effect is mediated by the ghrelin receptor (GHSR), with inhibition blocking ghrelin-induced and Ghsr knockdown , but not glutamatergic or ventral tegmental area cells, reducing females, independent circulating hormones. Female mice showed higher expression, neurons were more sensitive to ghrelin. Moreover, intra-EWcp delivery GHSR inverse agonist antagonist reduced drinking, suggesting direct actions These findings highlight critical mediator excessive consumption via mice, offering insights into system’s role

Language: Английский

Citations

1
Peptidergic and functional delineation of the Edinger-Westphal nucleus DOI Creative Commons
Michael F. Priest, Sara Nicole Freda, Isabelle J. Rieth

et al.

Cell Reports, Journal Year: 2023, Volume and Issue: 42(8), P. 112992 - 112992

Published: Aug. 1, 2023

Many neuronal populations that release fast-acting excitatory and inhibitory neurotransmitters in the brain also contain slower-acting neuropeptides. These facultative peptidergic cell types are common, but it remains uncertain whether neurons solely peptides exist. Our fluorescence situ hybridization, genetically targeted electron microscopy, electrophysiological characterization suggest most of non-cholinergic, centrally projecting Edinger-Westphal nucleus mice obligately peptidergic. We further show, using anterograde projection mapping, monosynaptic retrograde tracing, angled-tip fiber photometry, chemogenetic modulation ablation conjunction with canonical assays for anxiety, this population activates response to loss motor control promotes anxiety responses. Together, these findings elucidate an integrative, ethologically relevant role functionally align periaqueductal gray, where resides. This work advances our understanding provides a framework future investigations systems.

Language: Английский

Citations

19
A Sleep‐Specific Midbrain Target for Sevoflurane Anesthesia DOI Creative Commons
Tingting Yi, Na Wang, Jing Huang

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(15)

Published: March 24, 2023

Sevoflurane has been the most widely used inhaled anesthetics with a favorable recovery profile; however, precise mechanisms underlying its anesthetic action are still not completely understood. Here authors show that sevoflurane activates cluster of urocortin 1 (UCN1

Language: Английский

Citations

17
Neural and behavioral plasticity across the female reproductive cycle DOI
Nicolás Gutiérrez‐Castellanos, Basma Fatima Anwar Husain, Inês C. Dias

et al.

Trends in Endocrinology and Metabolism, Journal Year: 2022, Volume and Issue: 33(11), P. 769 - 785

Published: Oct. 14, 2022

Language: Английский

Citations

21
Imprinted genes and the manipulation of parenting in mammals DOI
Rosalind M. John, Matthew J. Higgs, Anthony R Isles

et al.

Nature Reviews Genetics, Journal Year: 2023, Volume and Issue: 24(11), P. 783 - 796

Published: Sept. 15, 2023

Language: Английский

Citations

12
Functionally active TRPA1 ion channel is downregulated in peptidergic neurons of the Edinger-Westphal nucleus upon acute alcohol exposure DOI Creative Commons

Ammar Al-Omari,

Miklós Kecskés, Balázs Gaszner

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 10

Published: Jan. 10, 2023

Introduction: The centrally projecting Edinger-Westphal nucleus (EWcp) contributes to the control of alcohol consumption by its urocortin 1 (UCN1) and cocaine- amphetamine-regulated transcript (CART) co-expressing peptidergic neurons. Our group recently showed that urocortinergic EWcp is primary seat central nervous system transient receptor potential ankyrin (TRPA1) cation channel mRNA expression. Here, we hypothesized metabolites, pass through blood-brain barrier, may influence function cells in activating TRPA1 ion channels. We aimed examine functional activity possible role a mouse model acute exposure. Methods: Electrophysiological measurements were performed on brain slices C57BL/6J male mice containing prove using selective, potent, covalent agonist JT010. Male knockout (KO) wildtype (WT) compared with each other morphological studies upon treatment. In both genotypes, half animals was treated intraperitoneally g/kg 6% ethanol vs. physiological saline-injected controls. Transcardial perfusion 2 h after area, FOS immunohistochemistry assess neuronal activation. TRPA1, CART, expression as well CART peptide content semi-quantified RNAscope situ hybridization combined immunofluorescence. Results: JT010 activated channels slices. Alcohol treatment resulted significant activation genotypes. decreased Trpa1 WT mice. assessment immunoreactivity revealed lower basal KO WTs. affected genotype-dependently alcohol: WTs while it increased exposure influenced neither nor EWcp/CART content. Conclusion: proved presence receptors neurons EWcp. Decreased treatment, associated reduced suggesting this contribute regulation release.

Language: Английский

Citations

11