A Hypothetical Protein Fragment from Large Yellow Croaker (Larimichthys crocea) Demonstrates Significant Activity Against Both Bacterial and Parasite DOI Creative Commons

Chunmei Yan,

Meiling Chen, Hao Xu

et al.

Fishes, Journal Year: 2025, Volume and Issue: 10(3), P. 109 - 109

Published: March 4, 2025

Antimicrobial peptides (AMPs) are biocompatible and biodegradable, making them an attractive alternative to traditional antimicrobial agents chemical preservatives. Here, a novel α-helix amphiphilic anionic AMP Lc149 was screened from large yellow croaker (Larimichthys crocea) using Bacillus subtilis expression system. is hypothesized protein fragment not annotated in the genome of croaker. Both extracellular recombinant (rLc149) exhibited significant killing effects against Gram-negative Escherichia coli Vibrio harveyi. Scanning transmission electron microscopy revealed that rLc149 had ability disrupt bacterial cell membranes, causing irregular morphology, severe membrane damage, cytoplasm agglutination, intracellular content leakage. Confocal laser scanning flow cytometry further confirmed destruction mortality rates over 80%. Gel retardation assays SDS-PAGE electrophoresis showed unable bind DNA, but did reduce contents. Additionally, maintained antibacterial activity E. V. harveyi upon exposure temperatures 25–100 °C, UV radiation time 0–60 min, pH levels 3–12, different proteases. Biosafety low hemolytic toxicity erythrocytes croaker, rabbit, shrimp, cytotoxicity kidney cells HEK 293T cells. More deeply, also possessed parasites. Therefore, can be considered antiparasitic drug fisheries.

Language: Английский

Modelling the effects of climate change on shellfish production in marine artisanal fisheries of Ghana DOI Creative Commons
Sandra Akugpoka Atindana,

Patrick K. Ofori‐Danson,

Sandra Brucet

et al.

Open Research Africa, Journal Year: 2025, Volume and Issue: 2, P. 16 - 16

Published: Jan. 16, 2025

Background Ghana’s marine artisanal fisheries, particularly the small pelagic are in a state of crisis. The decline number fish attributable to overfishing, climate variability and unsustainable fishing methods. Similarly, wake change, shellfishes (particularly oysters, scallops mussels) highly vulnerable. Methods A total 55 years’ worth data from fisheries were studied relation indices. primary objective was develop simple linear regression model for predicting shellfish catch Ghana. Key informant interviews employed soliciting on changes along coastline trends shell catch. Results predictor variable that significantly explained production temperature. Hence, is valuable tool predict future fisheries. Conclusions Increases sea surface temperature will adversely affect production. It therefore important Ministry Fisheries Aquaculture Development other stakeholders should, their decision-making processes, ensure formulation smart policies management strategies sustainable use resource.

Language: Английский

Citations

0
Loss and damage in tropical fisheries: a systematic review of people, climate, and fisheries DOI Creative Commons
Alicia N’Guetta, Emily Boyd, Torsten Krause

et al.

Regional Environmental Change, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 19, 2025

Language: Английский

Citations

0
A Hypothetical Protein Fragment from Large Yellow Croaker (Larimichthys crocea) Demonstrates Significant Activity Against Both Bacterial and Parasite DOI Creative Commons

Chunmei Yan,

Meiling Chen, Hao Xu

et al.

Fishes, Journal Year: 2025, Volume and Issue: 10(3), P. 109 - 109

Published: March 4, 2025

Antimicrobial peptides (AMPs) are biocompatible and biodegradable, making them an attractive alternative to traditional antimicrobial agents chemical preservatives. Here, a novel α-helix amphiphilic anionic AMP Lc149 was screened from large yellow croaker (Larimichthys crocea) using Bacillus subtilis expression system. is hypothesized protein fragment not annotated in the genome of croaker. Both extracellular recombinant (rLc149) exhibited significant killing effects against Gram-negative Escherichia coli Vibrio harveyi. Scanning transmission electron microscopy revealed that rLc149 had ability disrupt bacterial cell membranes, causing irregular morphology, severe membrane damage, cytoplasm agglutination, intracellular content leakage. Confocal laser scanning flow cytometry further confirmed destruction mortality rates over 80%. Gel retardation assays SDS-PAGE electrophoresis showed unable bind DNA, but did reduce contents. Additionally, maintained antibacterial activity E. V. harveyi upon exposure temperatures 25–100 °C, UV radiation time 0–60 min, pH levels 3–12, different proteases. Biosafety low hemolytic toxicity erythrocytes croaker, rabbit, shrimp, cytotoxicity kidney cells HEK 293T cells. More deeply, also possessed parasites. Therefore, can be considered antiparasitic drug fisheries.

Language: Английский

Citations

0