Composition of lipid nanoparticles for targeted delivery: application to mRNA therapeutics DOI Creative Commons
О. С. Васильева, Olga V. Zaborova,

Bogdan Shmykov

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 23, 2024

Today, lipid nanoparticles (LNPs) are some of the main delivery systems for mRNA-based therapeutics. The scope LNP applications in terms RNA is not limited to antiviral vaccines but encompasses anticancer drugs and therapeutics genetic (including rare) diseases. Such widespread use implies high customizability targeted LNPs specific organs tissues. This review addresses vector-free options LNPs, namely influence composition these on their biodistribution. In review, experimental studies examined that focused biodistribution mRNA or encoded protein after administration via mammals. We also performed a comprehensive analysis individual lipids’ functional groups ensure desired organs. These data will allow us outline prospects further optimization compositions

Language: Английский

Emerging Frontiers in acute kidney injury: The role of extracellular vesicles DOI
Sirui Li, Lan Zhou, Yu Huang

et al.

Bioactive Materials, Journal Year: 2025, Volume and Issue: 48, P. 149 - 170

Published: Feb. 18, 2025

Language: Английский

Citations

1
Nanoparticles transfected with plasmid-encoded lncRNA-OIP5-AS1 inhibit renal ischemia-reperfusion injury in mice via the miR-410-3p/Nrf2 axis DOI Creative Commons
Rongjie Zhang, Xin Zhang, Xuhui Zhu

et al.

Renal Failure, Journal Year: 2024, Volume and Issue: 46(1)

Published: Feb. 29, 2024

Nanostructures composed of liposomes and polydopamine (PDA) have demonstrated efficacy as carriers for delivering plasmids, effectively alleviating renal cell carcinoma. However, their role in acute kidney injury (AKI) remains unclear. This study aimed to investigate the effects plasmid-encoded lncRNA-OIP5-AS1@PDA nanoparticles (POP-NPs) on ischemia/reperfusion (RI/R) explore underlying mechanisms. RI/R or OGD/R models were established mice HK-2 cells, respectively. In vivo, vector POP-NPs administered (10 nmol, IV) 48 h after treatment. mouse model, OIP5-AS1 Nrf2/HO-1 expressions down-regulated, while miR-410-3p expression was upregulated. treatment reversed RI/R-induced tissue injury, restoring altered levels blood urea nitrogen, creatinine, malondialdehyde, inflammatory factors (IL-8, IL-6, TNF-α), ROS, apoptosis, miR-410-3p, well suppressed SOD model mice. Similar results obtained treated with POP-NPs. Additionally, mimics could reverse cellular models, partially counteracted by Nrf2 agonists. The binding relationship between alongside Nrf2, has been substantiated dual-luciferase reporter RNA pull-down assays. revealed that can attenuate through miR-410-3p/Nrf2 axis. These findings lay groundwork future targeted therapeutic approaches utilizing AKI.

Language: Английский

Citations

4
Loureirin C improves mitochondrial function by promoting NRF2 nuclear translocation to attenuate oxidative damage caused by renal ischemia–reperfusion injury DOI Creative Commons

Yucheng Qi,

Jinli Zheng,

Yuan Zi

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 138, P. 112596 - 112596

Published: July 8, 2024

Acute kidney injury (AKI) is a common clinical syndrome worldwide, with no effective treatment strategy. Renal ischemia-reperfusion (IR) one of the main AKI features, and excessive reactive oxygen species (ROS) production during reperfusion causes severe oxidative damage to kidney. Loureirin C (LC), an active ingredient in traditional Chinese medicine dragon's blood, possesses excellent antioxidative properties, but its role renal IR not clear. In this study, we evaluated protective effects LC against vivo vitro by establishing mice model human proximal tubular epithelial cell (HK-2) hypoxia/reoxygenation (HR) model. We found that ameliorated function tissue structure inhibited stress ferroptosis vivo. vitro, scavenged ROS attenuated mitochondrial dysfunction HK-2 cells, thereby inhibiting cellular injury. Furthermore, effectively promoted nuclear factor erythroid 2-related 2 (NRF2) translocation activated downstream target genes heme oxygenase 1 (HO-1) NADPH quinone oxidoreductase-1 (NQO-1) enhance antioxidant function. Moreover, NRF2 knockdown pharmacological inhibition partially eliminated effect LC. These results confirm can inhibit injury, mechanism may be associated activation

Language: Английский

Citations

4
Therapeutic nucleic acids in regenerative medicine and tissue repair DOI
Qimanguli Saiding, Duotian Qin, Soohwan An

et al.

Nano Research, Journal Year: 2024, Volume and Issue: 17(10), P. 8942 - 8976

Published: July 13, 2024

Language: Английский

Citations

4
mRNA therapy: A new frontier in regenerative medicine DOI Creative Commons
Danna Xue, Yue Zhang,

Anran Shen

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Abstract Tissue regeneration has raised intensive attention due to its great significance in overcoming various diseases resulting from different injuries. Since the COVID‐19 pandemic, mRNA therapeutics have emerged as innovative strategies prevention and treatment of their unique advantages. Compared traditional regenerative strategies, therapy offers rapid translation into proteins with low production cost high modifiability. Herein, we discuss progress key processes therapy, focusing on therapeutic modification delivery carriers. The preclinical clinical studies for cardiac, lung, liver, kidney, locomotor system, skin lesions neurological disorders were summarized comprehensively. Developing reduce immunogenicity off‐target effects, well optimization system may accelerate pace translation.

Language: Английский

Citations

0
Schisandrin B Regulates Mitochondrial Dynamics via AKT1 Activation and Mitochondrial Targeting to Ameliorate Renal Ischemia-Reperfusion Injury DOI

Changhong Xu,

Huabin Wang,

Hailong Wang

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 156672 - 156672

Published: March 1, 2025

Language: Английский

Citations

0
Toll-Like Receptors in Kidney Ischemia-Reperfusion Injury: Modulating Macrophage Responses for Therapeutic Insights DOI

H. Malathi,

Gaurav Khandelwal,

S. Gayathri

et al.

Pathology - Research and Practice, Journal Year: 2025, Volume and Issue: unknown, P. 155940 - 155940

Published: March 1, 2025

Language: Английский

Citations

0
Mitochondrial Extracellular Vesicles: A Promising Avenue for Diagnosing and Treating Lung Diseases DOI

Fengxia Ding,

Mi Zhou,

Yinying Ren

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(37), P. 25372 - 25404

Published: Sept. 3, 2024

Mitochondria, pivotal organelles governing cellular biosynthesis, energy metabolism, and signal transduction, maintain dynamic equilibrium through processes such as biogenesis, fusion, fission, mitophagy. Growing evidence implicates mitochondrial dysfunction in a spectrum of respiratory diseases including acute lung injury/acute distress syndrome, bronchial asthma, pulmonary fibrosis, chronic obstructive disease, cancer. Consequently, identifying methods capable ameliorating damaged function is crucial for the treatment diseases. Extracellular vesicles (EVs), nanosized membrane released by cells into extracellular space, facilitate intercellular communication transferring bioactive substances or signals between organs. Recent studies have identified abundant components within specific subsets EVs, termed (mitoEVs), whose contents compositions vary with disease progression. Moreover, mitoEVs demonstrated reparative functions injured recipient cells. However, comprehensive understanding currently lacking, limiting their clinical translation prospects. This Review explores classification, functional cargo, biological effects mitoEVs, focus on role Emphasis placed potential markers innovative therapeutic strategies diseases, offering fresh insights mechanistic drug development various disorders.

Language: Английский

Citations

3
Kynurenines as a Novel Target for the Treatment of Inflammatory Disorders DOI Creative Commons
Adrian Mor, Anna Tankiewicz‐Kwedlo,

Marianna Ciwun

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(15), P. 1259 - 1259

Published: July 26, 2024

This review discusses the potential of targeting kynurenine pathway (KP) in treatment inflammatory diseases. The KP, responsible for catabolism amino acid tryptophan (TRP), produces metabolites that regulate various physiological processes, including inflammation, cell cycle, and neurotransmission. These metabolites, although necessary to maintain immune balance, may accumulate excessively during leading systemic disorders. Key KP enzymes such as indoleamine 2,3-dioxygenase 1 (IDO1), 2 (IDO2), (TDO), 3-monooxygenase (KMO) have been considered promising therapeutic targets. It was highlighted both inhibition activation these be beneficial, depending on specific disorder. Several conditions, autoimmune diseases, which modulation activity holds promise, described detail. Preclinical studies suggest this an effective strategy diseases options are currently limited. Taken together, highlights importance further research clinical application enzyme development new strategies

Language: Английский

Citations

2
Nucleic acid-based nanotherapeutics for treating sepsis and associated organ injuries DOI Creative Commons
Huang‐Ping Yu, Fu-Chao Liu,

Yu-Kuo Chung

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(11), P. 4411 - 4437

Published: Jan. 1, 2024

In recent years, gene therapy has been made possible with the success of nucleic acid drugs against sepsis and its related organ dysfunction.Therapeutics based on acids such as small interfering RNAs (siRNAs), microRNAs (miRNAs), messenger (mRNAs), plasmid DNAs (pDNAs) guarantee to treat previously undruggable diseases.The advantage acid-based lies in development nanocarriers, achieving targeted controlled delivery for improved efficacy minimal adverse effects.Entrapment into nanocarriers also ameliorates poor cellular uptake naked acids.In this study, we discuss current state art nanoparticles hyperinflammation apoptosis associated sepsis.The optimized design through physicochemical property modification ligand conjugation can target specific organs-such lung, heart, kidney, liver-to mitigate multiple sepsis-associated injuries.This review highlights nanomaterials designed fabricating anti-sepsis nanosystems, their characterization, mechanisms working sepsis, potential promoting therapeutic efficiency acids.The investigations nanoparticulate application management are summarized paper.Noteworthily, nanotherapeutic allows a novel strategy sepsis.Further clinical studies required confirm findings cell-and animal-based experiments.The capability large-scale production reproducibility nanoparticle products critical commercialization.It is expected that numerous possibilities will be investigated nanotherapeutics future.

Language: Английский

Citations

2